atlas of light-ct images (pathology)

7/29/2019 Atlas of Light-CT images (pathology)

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LLTech

Light-CT Scanner

Atlas of images

Clinical applications


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TABLE OF CONTENTS

BREAST Page 3

SKIN Page 10

CORNEA Page 14 PROSTATE Page 17

LUNG Page 22

KIDNEY Page 26

BRAIN Page 29

HEAD & NECK Page 34

CLINICAL APPLICATIONS


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- Extemporaneous analysis for cancer surgery

- Biobanking selection

Back to Table of Contents

BREAST AND LYMPH NODES


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Grainy aspect of normal

fibrous tissue

Duct with

calcification

Lobule Adipocytes Vessel

Healthy breast tissue

Courtesy of Hpital Tenon, Paris, FranceAssayag et al, TCRT Express 1(1):e600254


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Ductal Carcinoma In Situ (DCIS)

Enlarged lobule

Duct with necrosis

Histology and LightCT images reveal the enlarged abnormal lobules and ducts characteristic of DCIS

Assayag et al, TCRT Express 1(1):e600254


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Lobular carcinoma

In-situ carcinoma cells

Invasive carcinoma cells

1 mm100 m

100 m

Tumorous cells are visible in in-situ and invasive carcinoma


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100m

Highly scattering

thin trabeculae

aspect: fibrous

tissue in the

tumourous part

Grainy medium

scattering aspect:

healthy fibrous

tissue

Invasive adenocarcinoma: nodular tumor

Interface of the tumourous and normal

appearing part :

Grey zones corresponding to foci

of carcinomatous cells

Tumour margin

500m

500m

Courtesy of Hpital Tenon, Paris, France

Assayag et al, TCRT Express 1(1):e600254


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Nodules containing

lymphoid tissue

Normal Sentinel Node

Adipocytes in hilum

Capsule Grieve et al Proc SPIE 2013


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Lymphoid zone

Invaded zone

Invaded Sentinel Node

Bright white aspect,

highly scattering dense

collagen meshwork

Grieve et al Proc SPIE 2013


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- Extemporaneous margin analysis for cancer

surgery- Biobanking selection


SKIN


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Epidermis

Collagen

Pilosebaceous

unit

Sweat gland

Adipocytes

b

a

c

e

d

500 m

Normal skin morphology: vertical section

Dalimier and Salomon Dermatology 224, 84-92 (2012)


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Skin pathologies: basal cell carcinoma discrimination

at structural level

Dense peritumoral stroma

Dalimier and Salomon Dermatology 224, 84-92 (2012)


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Zoom on basal cell carcinoma:

discrimination at cellular level

200 m

100 m

Peritumoral stroma

High cell density

Courtesy of Hopitaux Universitaires de Genve, Genve, Switzerland


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CORNEA


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Surface epithelium Intermediary epithelium Basal epithelium

Bowmans layer Stromal keratocytes Endothelial cells

Cross sectional view En face views

Normal human cornea

100 m 100 m

LightCT imaging reveals the structures of normal cornea: epithelium, Bowmans layer, stroma, Descemets membrane,

and endothelium.

Use of LightCT in Eye Banks would allow graft cornea integrity to be assessed prior to transplant.Current practice examines only the epithelial surface and endothelial cells under a traditional specular microscope.


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Subepithelial fibrosis

Intraepithelial edema

LightCT cross section LightCT en face

Cornea with bullous keratopathy

Sub-epithelial

fibrosis

Optovue OCT in vivo on patientHistology

Histology, conventional OCT and Light CT views of the same region are compared.

The improvement in resolution with LightCT compared to conventional OCT is appreciated.

Pathological areas of subepithelial fibrosis and intraepithelial edema are identified.

Bowmans layer is clearly visible under the area of fibrosis.

150 m 150 m 100 m

Ghouali et alARVO (2013)


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- Biobanking selection- Biopsy quality assessment


PROSTATE


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1 mm

Human prostatic glands with hyperplasia


Zoom on hyperplasic gland

100 m


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3D imaging of glands

Stack of 150 images with 1 m step

50 m

50 m

50 m

sagittal

coronal

oblique

Sagittal reconstruction

Coronal reconstruction

Oblique reconstruction



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1 mm

1 mm

Biopsy imaging

Courtesy of Hpital Cochin, Paris, France


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Tumorous glands

200 m

Courtesy of Hpital Cochin, Paris, France


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LUNG


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Cross section of rat lung

Jain et al.J Pathol Inform 2011, 2:28.Courtesy of Weill Medical College, NY, USA

500 m


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Non-neoplastic human lung tissue

Alveoli

Blood vessel

Collapsed lung

Jain et al. Pathology Visions 2013.

Courtesy of Weill Medical College, NY, USA

500 m


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Adenocarcinoma of lung with lepidic & invasive

components

Lepidic

Invasive Invasive

Lepidic

InvasiveInvasive

500 m

100 m 100 m



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KIDNEY


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Non-neoplastic kidney tissue

Glomerulus

Tubules

Blood

vessel

Caron et al. Proc SPIE 2013

Blood vesselGlomerulus

Tubules

200 m


100 m

100 m

100 m


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Chromophobe renal cell carcinoma

Sheets of cells

Normal

architecture is

replaced by

sheets of cells

Sheets of cells


100 m

50 m


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- Biobank selection

- In-vivo potential for surgical guidance


BRAIN AND SPINAL CORD

LightCT imaging of human epileptic brain and cerebellum identifies myelinated


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Neuron cell bodies (dark dots)

Axons/myelin fibers

Capillary

Cortex

White matter

Axon bundles

LightCT imaging of human epileptic brain and cerebellum identifies myelinated

axon fibers, a subpopulation of neuronal cell bodies and CNS vasculature.

Varlet et al 2013(submitted)

50 m

50 m

80 m

500 m

50 m

80 m

50 m

Hippocampus: Coronal section (left side)


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CA4 field

Hippocampal sulcus

Alveus

Sub-Ependymal

zone

CA1 field of the cornus ammonis

Stratum radiatus

of the CA1 field

Stratum granulosum of the dentate gyrus

Pyramidal neurons of the CA4 field

responsible for memory

Hippocampus: Coronal section (left side)

900 m

40 m

40 m

80 m

80 m


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Choroid Plexus Papilloma : Xanthomatous changes

Characteristic cauliflower-like masses

Empty papilla

Papilla filled with blood

Single layer of plexus cellsRare (0.5%) benign tumors

Diagnosis possible directly on FF-OCT imageVarlet et al 2013 (submitted)

50 m

50 m

20 m150 m

50 m

50 m

20 m


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LightCT images allow the distinction of meningiomas

from meningeal haemangiopericytoma

Collagen bundles

Whorls

Calcification

Collagen balls

Meningioma psammoma diagnosis possible directly on FF-OCT image

80% of meningiomas are benign.

Histologically, meningioma cells are relatively uniform, with a tendency to encircle one another, forming

whorls and psammoma bodies (laminated calcific concretions). They have a tendency to calcify and are

highly vascularized. Areas of fibrosis may be present.Varlet et al 2013 (submitted)

50 m

10 m

10 m500 m


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- Extemporaneous margin analysis forcancer surgery

- In-vivo potential for surgical guidance


HEAD AND NECK TISSUE

Gl t500 m


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Glossectomy

Tumorous tongue tissue

Courtesy of Institut Gustave Roussy, Villejuif, France

Thin trabeculae aspect of

the fibrous tissue

Cell nuclei1 mm

500 m 500 m

Gl t


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Glossectomy

Mouth floor

Courtesy of Institut Gustave Roussy, Villejuif, France

Epithelial cell

nuclei

Spinous cells

1 mm

500 m 500 m

500 m


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atlas of light-ct images (pathology)

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