automatic fiuorescence and brightﬁeld microscope: tomorrow ... · automatic fiuorescence and...

Automatic FIuorescence and Brightfield Microscope:

Tomorrow's Technology for Today's Science.

Bio Imaging Navigator

• Illumination devices for microscope have suggested lifetimes. Periodic inspections are required. Please visit our website for details.• All company and product names are registered trademarks and/or trademarks of their respective owners.• Images on the PC monitors are simulated.• Specifications and appearances are subject to change without any notice or obligation on the part of the manufacturer.

• OLYMPUS CORPORATION is ISO14001 certified.• OLYMPUS CORPORATION is FM553994/ISO9001 certified.

Printed in Japan M1675E-032015

Shinjuku Monolith, 2-3-1 Nishi-Shinjuku, Shinjuku-ku, Tokyo 163-0914, Japan

Wendenstrasse 14-18, 20097 Hamburg, Germany

491B River Valley Road, #12-01/04 Valley Point Office Tower, Singapore 248373

3 Acacia Place, Notting Hill VIC 3168, Australia

48 Woerd Avenue, Waltham, MA 02453, U.S.A.

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A8F, Ping An International Financial Center, No. 1-3, Xinyuan South Road, Chaoyang District, Beijing, 100027 P.R.C.

8F Olympus Tower, 446 Bongeunsa-ro, Gangnam-gu, Seoul, 135-509 Korea

FSX100 delivers superb image quality andexceptional operability.

FSX100 is highly regarded for its sophisticated performance that opens new horizons to digital imaging,It integrates the advantages of outstanding operability and

extremely high image quality that are hallmarks of the Olympus brand. In addition,the FSX100 boasts refinements and performance enhancements over traditional microscopes that support imaging

from 4.2x up to 80x, and extensive capabilities that enable the observation of a wide variety of specimens.The FSX100 is engineered to keep pace with today's demands and tomorrow's expectations of scientific and medical research.

FSX100 – bringing bio-imaging into a whole new focus.

FSX100 delivers superb image quality andexceptional operability.

FSX100 is highly regarded for its sophisticated performance that opens new horizons to digital imaging,It integrates the advantages of outstanding operability and

extremely high image quality that are hallmarks of the Olympus brand. In addition,the FSX100 boasts refinements and performance enhancements over traditional microscopes that support imaging

from 4.2x up to 80x, and extensive capabilities that enable the observation of a wide variety of specimens.The FSX100 is engineered to keep pace with today's demands and tomorrow's expectations of scientific and medical research.

FSX100 – bringing bio-imaging into a whole new focus.

Step1Step1 Step2Step2 Step3Step3 Step4Step4

No training is necessary with the easy-to-use workflow.Even first-time users can capture high quality imagessimply, smoothly, and successfully.

Four-step Easy Operation

Bookmark

The available Noise Reduction Algorithm helps remove fluorescence blur and emphasize edges on the live images. Noise reduction is available in three levels according to the specimen type and your particular needs.

With Direct Overlay, an advanced and powerful feature of the FSX100, just one click allows you to overlay captured images over a live image. Direct Overlay is particularly useful with multiple color fluorescence specimens.

Real-time Noise Reduction Direct Overlay

Specimen Movement

Select ObservationMode

Select StartingPosition Frame Snap

Simply follow the intuitive software workflow to capture high quality images.

Choose a region forcloser examination.

Recall

Indicates One-click Position

Capture images efficiently, simply by clicking on bookmarked regions on the wide-field image.

The specimen can be moved in any X/Y direction by clicking and dragging the cursor on the screen, or by double-clicking a target area to center it in the window.

Specimen Movement

Super-easyOperation

After Noise Reduction

Before Noise Reduction

Green alga Botryococcus brauniiMisako Kato and Rika Niitsu, Plant Physiology, Graduate School of Humanities and Science,Ochanomizu University.

Step1Step1 Step2Step2 Step3Step3 Step4Step4

No training is necessary with the easy-to-use workflow.Even first-time users can capture high quality imagessimply, smoothly, and successfully.

Four-step Easy Operation

Bookmark

The available Noise Reduction Algorithm helps remove fluorescence blur and emphasize edges on the live images. Noise reduction is available in three levels according to the specimen type and your particular needs.

With Direct Overlay, an advanced and powerful feature of the FSX100, just one click allows you to overlay captured images over a live image. Direct Overlay is particularly useful with multiple color fluorescence specimens.

Real-time Noise Reduction Direct Overlay

Specimen Movement

Select ObservationMode

Select StartingPosition Frame Snap

Simply follow the intuitive software workflow to capture high quality images.

Choose a region forcloser examination.

Recall

Indicates One-click Position

Capture images efficiently, simply by clicking on bookmarked regions on the wide-field image.

The specimen can be moved in any X/Y direction by clicking and dragging the cursor on the screen, or by double-clicking a target area to center it in the window.

Specimen Movement

Super-easyOperation

After Noise Reduction

Before Noise Reduction

Green alga Botryococcus brauniiMisako Kato and Rika Niitsu, Plant Physiology, Graduate School of Humanities and Science,Ochanomizu University.

Superb ImageQuality Even illumination facilitates image capture, analysis and stitching.

Capture brilliant, high resolution images from low to highmagnification, and anywhere in between.

Projection phase contrast is adapted to effect phase contrast microscopy on the FSX100. In fluorescence mode, the phase plate is retracted from the optical path thus permitting maximal fluorescence capture by the objective and camera.

Optical Zoom Even Illumination

NA0.95 40x Objective Lens

High Transmittance Overa Wide Range of Wavelengths

Projection Phase Contrast

Large optical zoom lens maintains brightness and resolution across the entire magnification range.

Even in low magnification, illumination is even all the way to the edges of the image.

The standard 40x objective lens boasts the world’s highest N.A. 0.95 for exceptional brightness and resolution.

300 400 500 600 700 800 900 1000

Wavelength (nm)

40x Objective Transmittance

Superb ImageQuality Even illumination facilitates image capture, analysis and stitching.

Capture brilliant, high resolution images from low to highmagnification, and anywhere in between.

Projection phase contrast is adapted to effect phase contrast microscopy on the FSX100. In fluorescence mode, the phase plate is retracted from the optical path thus permitting maximal fluorescence capture by the objective and camera.

Optical Zoom Even Illumination

NA0.95 40x Objective Lens

High Transmittance Overa Wide Range of Wavelengths

Projection Phase Contrast

Large optical zoom lens maintains brightness and resolution across the entire magnification range.

Even in low magnification, illumination is even all the way to the edges of the image.

The standard 40x objective lens boasts the world’s highest N.A. 0.95 for exceptional brightness and resolution.

300 400 500 600 700 800 900 1000

Wavelength (nm)

40x Objective Transmittance

Fluorescence Lamp

Darkroom-freeWhen you close the front cover, the FSX100 creates its own dark room environment shielding the specimen from ambient light. Unlike with conventional microscopes, you can now perform fluorescence imaging in the open laboratory with the lights on.

Space SavingThe clean, sleek outer housing and compact optical design afford the FSX100 its small footprint. The FSX100 can be installed in locations previously considered too small for a conventional microscope.

No Lamp to Change with The Transmitted LED Light IlluminationThe LED transmitted light source used for phase contrast and brightfield imaging has a life expectancy of approximately 16,000 hours. Users will realize consistent performance for years with this white LED system.

Long-life Fluorescence LampThe pre-centered, 2,000-hour fluorescence excitation lamp system in the FSX100 is designed for stable performance and easy bulb replacement. Intelligent lamp design notifies the user when the bulb is nearing the end of its life cycle.

A series of images can be captured along the Z-axis(X and Y position is fixed) by defining the change in step between images. The full focus function merges these images to provide a clear, sharp, high focal depth image of the specimen - perfect for thick specimens.

No Darkroom Needed & Space SavingZ-stack Mode

Choose from a variety of image display options in the library management function. Individual image files can be easily accessed even in a multiple user environment.

Image Management

Extended Life-span Design

Image acquisition settings, such as exposure time, fluorescence channel and magnification can be recalled by simply clicking a previously captured image.This feature is useful for comparative experiments.

Imaging Setting Restore

Follow the logical workflow to capture images efficiently. Easily access and manage images with the built-in archive.Recall acquisition settings to save time.

Labor-savingWork Flow

ImageSetting is

Reproduced

Acquisition Image Click

Fluorescence Lamp

Darkroom-freeWhen you close the front cover, the FSX100 creates its own dark room environment shielding the specimen from ambient light. Unlike with conventional microscopes, you can now perform fluorescence imaging in the open laboratory with the lights on.

Space SavingThe clean, sleek outer housing and compact optical design afford the FSX100 its small footprint. The FSX100 can be installed in locations previously considered too small for a conventional microscope.

No Lamp to Change with The Transmitted LED Light IlluminationThe LED transmitted light source used for phase contrast and brightfield imaging has a life expectancy of approximately 16,000 hours. Users will realize consistent performance for years with this white LED system.

Long-life Fluorescence LampThe pre-centered, 2,000-hour fluorescence excitation lamp system in the FSX100 is designed for stable performance and easy bulb replacement. Intelligent lamp design notifies the user when the bulb is nearing the end of its life cycle.

A series of images can be captured along the Z-axis(X and Y position is fixed) by defining the change in step between images. The full focus function merges these images to provide a clear, sharp, high focal depth image of the specimen - perfect for thick specimens.

No Darkroom Needed & Space SavingZ-stack Mode

Choose from a variety of image display options in the library management function. Individual image files can be easily accessed even in a multiple user environment.

Image Management

Extended Life-span Design

Image acquisition settings, such as exposure time, fluorescence channel and magnification can be recalled by simply clicking a previously captured image.This feature is useful for comparative experiments.

Imaging Setting Restore

Follow the logical workflow to capture images efficiently. Easily access and manage images with the built-in archive.Recall acquisition settings to save time.

Labor-savingWork Flow

ImageSetting is

Reproduced

Acquisition Image Click

Floating section of the brain.Noriko Osumi, Emiko Yamanishi, Division of Developmental Neuroscience, Tohoku University Graduate School of Medicine.

Wide-areaStitching

Mode Image capture and stitching processes are completed automatically.　Generate highly refined, wide-area images at 4.2ｘ magnificationsimply by pressing the START button.

Selection of Imaging Zone Continuous Imaging Automatic Image StitchingThe focus position changes automatically with the registered focus points to capture images continuously.

The processes from image capture through generation of the final stitched image can be fully automated.

Image Analysis (Binarization)

Stitching-processed wide-area images can be stored with full resolution.High-resolution images display the entire field of view in sharp focus, and are useful for area determination, distribution,pattern recognition, and other analyses.

Option

Simply set the threshold, and a binarization of the image is generated. Area, area ratio and counts can be performed.

Define the imaging zone by selecting the peripheral points that you want to include. In addition, you can register specific focus points as desired.

Floating section of the brain.Noriko Osumi, Emiko Yamanishi, Division of Developmental Neuroscience, Tohoku University Graduate School of Medicine.

Wide-areaStitching

Mode Image capture and stitching processes are completed automatically.　Generate highly refined, wide-area images at 4.2ｘ magnificationsimply by pressing the START button.

Selection of Imaging Zone Continuous Imaging Automatic Image StitchingThe focus position changes automatically with the registered focus points to capture images continuously.

The processes from image capture through generation of the final stitched image can be fully automated.

Image Analysis (Binarization)

Stitching-processed wide-area images can be stored with full resolution.High-resolution images display the entire field of view in sharp focus, and are useful for area determination, distribution,pattern recognition, and other analyses.

Option

Simply set the threshold, and a binarization of the image is generated. Area, area ratio and counts can be performed.

Define the imaging zone by selecting the peripheral points that you want to include. In addition, you can register specific focus points as desired.

ImagesCapturedby Users

High resolution and high Signal-to-noise images.

Kidney glomerulus.Shigenari Arita, Division of Pathology, Chiba Children's Hospital.

Mouse hippocampal primary neuron (P0)Hyeon-Cheol Lee, Takao Inoue, Hiroyuki Arai, Department of Health Chemistry,Graduate School of Pharmaceutical Science, The University of Tokyo.

NG108-15 cellLeaf of the moss Sphagnum palustre L.Misao Itouga, RIKEN Plant Science Center,Plant Productivity Systems Research Group.

Leaf of the moss Sphagnum palustre L.Misao Itouga, RIKEN Plant Science Center,Plant Productivity Systems Research Group.

M phase of HeLa (ATCC) cell, Giemsa stain Satoshi Yamazaki, Sayuri Ito, Hisao Masai, Genome Dynamics Project,Department of Genome Medicine, Tokyo Metropolitan Institute of Medical Science.

HeLa(ATCC)-Fucci stably expressing cell lineSatos ki, Sayuri Ito, Hisao Masai, Genome Dynamics Project,Department of Genome Medicine, Tokyo Metropolitan Institute of Medical Science.

Mouse iPS cellsKenji Kitajima, Takahiko Hara, Stem Cell Project,Tokyo Metropolitan Institute of Medical Science.

Human periodontal ligament fibroblasts (cultured on honeycomb-patterned film)Nagayoshi Iwama, Division of Periodontology and Endodontology,Tohoku University Graduate School of Dentistry; Organized Polymer Materials,Institute of MultidisciplinaryResearch for Advanced Materials, Tohoku University

Midbrain of mouse embryo at day 12Yasunori Miyamoto, Animal Molecular Cell Biology, Graduate School of Humanitiesand Sciences, Ochanomizu University.

Papillary cells from thyroid gland aspiration