automating the cell culture sampling process
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Automating the Cell Culture Sampling Process. Mike Phipps Tara Ryan BME 273 February 11, 2002. Problem. Cell cultures maintained in bioreactors for Research and Development purposes in pharmaceutical companies must be sampled at least once daily - PowerPoint PPT PresentationTRANSCRIPT
Automating the Cell Culture Sampling Process
Mike Phipps
Tara Ryan
BME 273
February 11, 2002
Problem
• Cell cultures maintained in bioreactors for Research and Development purposes in pharmaceutical companies must be sampled at least once daily
• methods of manually withdrawing a sample from the bioreactor can be reliable but still come with risks of culture contamination
• lab workers must be trained and experienced in sterile technique
Existing Sampling Methods
Hot plate to maintain temperature
Sampling syringe
ethanol
DO sparger Temperatureprobe
pH probeAgitator
Existing Sampling Methods
ethanol
Sam
plin
g po
rt
Sampling syringe
3-way valve
Water gasket for temperature control
Water inWater out
DO spargerTemperature probe
pH probeagitator
Project Goals
• reduce the risk of contamination that occurs due to sampling
• reduce the time it takes a lab worker to draw a sample from a culture
• reduce the skill and training required by a lab worker
Design IdeasIdea #1
• Continuous flow of medium and cells through tubing loop
• switch 3-way valve to the sampling line in order to draw a sample
• simple
• does not avoid the traditional syringe switch
Design IdeasIdea #2
• Ethanol and wash sterilize the syringe tip (needle)
• Use of septa
• Expand to a set of 4 bioreactors
Design IdeasIdea #3
• Open flame sterilizes the syringe tip (needle)• Use of septa• Water-gasket bioreactor system for better maintenance of the
culture’s temperature• Expand to a set of 4 bioreactors
Design IdeasIdea #4
• Simpler (fewer steps for mechanical arm)
• Reliance on hood to provide sterility
• Expand to a set of 4 bioreactors
Future Work
• Selecting the final design (possibly a combination of the ideas presented)
• Calculations (heat transfers, air flows, etc.) to determine specifications of the final design
• Draw final design using AutoCAD
• Production of a prototype?
References
• ABEC Website, <http://www.abec.com>
• B. Braun Biotech Website, <http://www.bbraunbiotech.com>
• Bailey, James E., and Ollis, David F. Biochemical Engineering Fundamentals. McGraw-Hill Inc.: St. Louis, 1986.
• Balcarcel, R. Robert. Associate Professor of Chemical Engineering, Vanderbilt University.
• New Brunswick Scientific Website, <http://www.nbsc.com>
• Todar, Kenneth. “The Control of Microbial Growth.” 21 September 2000 <http://www.bact.wisc.edu/microtextbook/ControlGrowth/sterilization.html>