awacss (evk1-ct-2000-00045)envsens, lecce, italy, 2002 awacss dr. dale m. willard, universität...
Post on 15-Jan-2016
213 views
TRANSCRIPT
A
S
C
A
SAWACSS (EVK1-CT-2000-00045) ENVSENS, Lecce, Italy, 2002
AWACSSAWACSS
Dr. Dale M. Willard, Universität Tübingen (Gauglitz Group)Contract #: EVK1-CT-2000-00045
Duration: 3 Year, RTD Project (Starting March, 2001)Visit: http://barolo.ipc.uni-tuebingen.de/awacss
A
S
C
A
S
Automated Water Analyser Computer Supported System
A
S
C
A
SAWACSS (EVK1-CT-2000-00045) ENVSENS, Lecce, Italy, 2002
MotivationMotivation
More Effective Water Management More Effective Water Management Requires:Requires:
• Cost-effective, continuous monitoring.Cost-effective, continuous monitoring.
• Network monitoring for trend analysis Network monitoring for trend analysis and early warning.and early warning.
• Automated and rugged equipment.Automated and rugged equipment.
Pollution of ground, surface and Pollution of ground, surface and river water byriver water by
• AgricultureAgriculture
• Industry & MiningIndustry & Mining
• TrafficTraffic
• Boating, Sports, TourismBoating, Sports, TourismToday's State of Monitoring:Today's State of Monitoring:
• Standard analytical methods utilised, Standard analytical methods utilised, such as HPLC and MS.such as HPLC and MS.
• Time consuming, trained technicians, Time consuming, trained technicians, automation very difficult.automation very difficult.
• Ever increasing list of contaminants.Ever increasing list of contaminants.
A
S
C
A
SAWACSS (EVK1-CT-2000-00045) ENVSENS, Lecce, Italy, 2002
Goal: Develop a cost-effective, on-line, water monitoring Goal: Develop a cost-effective, on-line, water monitoring biosensor that will measure a variety of small organic pollutants biosensor that will measure a variety of small organic pollutants
in short-time with remote control and surveillance.in short-time with remote control and surveillance.
Expanded multi-analyte analysis capability allowing for up to 32 simultaneous analyte measurements. Immunochemical reagents are being developed for a host of small organic water pollutants.
Novel design approaches to the optical detection and fluidics including miniturized integrated optics and microfluidics. Reduced reagent volumes. Limits of detection matching EU prescribed requirements (~1 ppb).
Intelligent remote surveillance and control that will allow for unattended continuous monitoring. Instruments will be rugged and easy to handle by untrained personnel.
1
2
34
5
6
78 910
Monitoring of EstuariesOrigin of Pollution
Monitoring of CoastsDirection of Pollution
Monitoring of Side StreamsOrigin of Pollution
Monitoring ofIndustrial PlantsContamination
A
S
C
A
SAWACSS (EVK1-CT-2000-00045) ENVSENS, Lecce, Italy, 2002
Methodology: Immunoassay coupled with Total Internal Methodology: Immunoassay coupled with Total Internal Reflection FluorescenceReflection Fluorescence
Laser
IO-ChipWaveguide
Detection
PC
SamplesWaste
SignalCollection
Fluidics
Analyte derivative
Analyte
Antibody
Fluorophor
Analyte derivative
Analyte
Antibody
Fluorophor
Analyte
Antibody
Fluorophore
* *
Flow
Transducer
* *
Flow
Remaining free antibodies arecaptured and detected.
* *
Flow
* *
FlowSamples are pre-incubatedwith labelled antibodies.
A
S
C
A
SAWACSS (EVK1-CT-2000-00045) ENVSENS, Lecce, Italy, 2002
AWACSS TechnologyAWACSS Technology
• Miniaturisation
(μL volumes)• Quick Analysis
(ca. 15 min.)• Multi-analyte Detection
2D Array Detection
32 Analysis Spots
Microfluidics
Integrated Optics
A
S
C
A
SAWACSS (EVK1-CT-2000-00045) ENVSENS, Lecce, Italy, 2002
Active Areas of WorkActive Areas of Work
InstrumentDesign
Immuno-chemistry
MarketSurvey &Tutorial
WaterMonitoring
Software& Network
IntegratedOptical
Chip
A
S
C
A
SAWACSS (EVK1-CT-2000-00045) ENVSENS, Lecce, Italy, 2002
Instrument DesignInstrument Design
1st Year Goal: Design and order the equipment necessary to assemble the AWACSS instruments.
Progress: Microfluidics designed with preliminary testing: small volumes & Poiseuille flow.
Designed or selected pumps, valves, lasers, detectors, power supplies, filters, circuit boards, computers.
Autosampler chosen.
Preliminary testing of electronics.
A
S
C
A
SAWACSS (EVK1-CT-2000-00045) ENVSENS, Lecce, Italy, 2002
Integrated-Optical (IO)-Chip DevelopmentIntegrated-Optical (IO)-Chip Development
Goal: Develop IO-Chips capable of simultaneously illuminating 32 analysis windows in a miniaturised format.
Advantages:
• Single mode waveguides allow for efficient and consistant analysis window illumination.
• Compact dimensions allow for microfluidic design and reduce sample & reagent volumes.
• Spatially defined but resolved analysis windows allow efficient detection and suppress signal crosstalk.
Progress: Protype IO-Chips have been designed and fabricated. Preliminary tests are now being carried out.
W a v e g u id e In le t
A n a ly sis W in d o w s (3 0 0 m x 1 .5 m m )
A
S
C
A
SAWACSS (EVK1-CT-2000-00045) ENVSENS, Lecce, Italy, 2002
Software and NetworkSoftware and Network
Local station
Local Stations
• Multi-analyte detection
• Data acquisition
• Data evaluation
• Local station control
Control Station
• Global survey and early warning
• Global trend analyses
• Local station control
• Detection limit control
prescribed by EU Directives Local station
Progress:• Measurement station software modules are selected and requirements are defined.• The first generation software simulator is complete.• The network design is defined.
A
S
C
A
SAWACSS (EVK1-CT-2000-00045) ENVSENS, Lecce, Italy, 2002
ImmunochemistryImmunochemistry
Transducer
* *
Flow
* *
Flow
* *
Flow1) Synthesis and Isolation of Antibodies and Analyte Derivatives.• Polyclonal from Livestock• Antibodies Isolated with ELISA
2) Assay Development.• Binding efficiency• Cross-reactivity• Fluorescently labelled
3) Transducer Spotting & Final Testing.• Spotting protocol established• Final Testing will conclude project
A
S
C
A
SAWACSS (EVK1-CT-2000-00045) ENVSENS, Lecce, Italy, 2002
Targeted Analytes under DevelopmentTargeted Analytes under Development
Herbicides2,4-DAtrazineSimazineIsoproturonDiuronFenuronMethoxuronAlachlorPropanilTrifluralinGlyphosate
FungicidesPentachlorophenolPentachlorobenzene
InsecticidesChlorpyrifosEndosulfan
Endocrine Disrupting CompoundsSteriod Estrogens: Progesterone, EstradiolBisphenol AIrgarolNonylphenol
AntibioticsSulphonamideTetracyclines
Suspected CarcinoginsPyreneTrichloroethyleneBTEXDEHP
Industrial Wastes/Chemical MarkersMTBESulphonate DetergentsCaffeine
Assays in Phase 2
A
S
C
A
SAWACSS (EVK1-CT-2000-00045) ENVSENS, Lecce, Italy, 2002
Water MonitoringWater Monitoring
IIQAB, Spain(Llobregat River)
TZW, Germany(Rhine River)
EI, Slovak Republic(Nitra River)
Goals:• Cataloguing and monitoring local water quality using standard methods (HPLC). Data helps community and project establish trends and identify problems.• Establishing monitoring sites for final instruments.• Defining requirements of end-users, validating design issues, and testing final instruments.
A
S
C
A
SAWACSS (EVK1-CT-2000-00045) ENVSENS, Lecce, Italy, 2002
Water Monitoring ProgressWater Monitoring Progress
• Cross-referencing exercise was performed and standardised protocols for sampling, storing, shipment, and extraction developed.
• Target sites for field deployment: Saint Joan Despi (Llobregat River, Spain), Worms (Rhine River, Germany), Novaky Chemical Plant (Nitra River, Slovak Republic).
• Data Collection: 12 measurement sites, 36 compounds9 compounds recorded significant levels
0,00
0,02
0,04
0,06
0,08
0,10
0,12
21.12.1998 09.07.1999 25.01.2000 12.08.2000 28.02.2001 16.09.2001 04.04.2002
c in
µg
/L Karlsruhe
Mainz
Düsseldorf
Atrazine Conc. in Rhine over 3 Years
• Matrix Effect Studies:
Turbity, Temperature,pH, Ionic Strength
0
5
10
15
20
25
30
tem
pe
ratu
re i
n °
C
jan 2001 dec 2001
Temperature of Rhine through Calender Year
5 °C5 °C
25 °C25 °C
A
S
C
A
SAWACSS (EVK1-CT-2000-00045) ENVSENS, Lecce, Italy, 2002
Other ResultsOther Results
Market Analysis
• Survey conducted of European and American water monitoring programs.
• Survey found total micropollutant water sample examination market to be >30 million €. Information broken down according to regions and compounds.
• Water Framework Directive 2000/60/EC is found as bench mark legal act. (13 AWACSS compounds are listed as "dangerous substances".)
Multimedia Tutorial
• Efficient Training
• Cost-savings
A
S
C
A
SAWACSS (EVK1-CT-2000-00045) ENVSENS, Lecce, Italy, 2002
PartnersPartnersEberhard-Karls-University Tübingen: Project Management and Surface Immunoassay Development
Central Research Laboratory: Instrument Hardward Design
Siemens: Electronics, Internet Communication, and Market Analysis.
Optoelectronics Research Centre: Integrated Optical Chips
Environmental Institute, Kos: Instrument Design and Field Tests-Slovakia
King‘s College London: Immunochemistry
Institute of Chem. & Environ. Chem. IIQAB: Instrument Design and Field Tests-Spain
DVGW-Technologiezentrum Wasser: Instrument Design and Field Tests-Germany
Funding: This Project is funded under the 5th Framework Programme: Energy, Environment, andSustainable Development. Visit: http://barolo.ipc.uni-tuebingen.de/awacss.