awesome lecture#2 microfluidics + integrated circuit can control cells and chemical compounds
TRANSCRIPT
AWESOME LECTURE#2
Microfluidics + integrated circuit can control cells and chemical
compounds.
MICROFLUID +
INTEGRATED CIRCUIT =
Overview…
Overview… What are microfluids?
Overview… What are microfluids? Lab on a Chip (control)
Overview… What are microfluids? Lab on a Chip (control) Tests & Applications
SMALL FLUIDS!
SMALL FLUIDS! Channel < 1 mm in length
SMALL FLUIDS! Channel < 1 mm in length
Examples:
SMALL FLUIDS! Channel < 1 mm in length
Examples:
SMALL FLUIDS! Channel < 1 mm in length
Examples:
SMALL FLUIDS! Channel < 1 mm in length
Examples:
SMALL FLUIDS! Channel < 1 mm in length
Examples:
Interesting properties…
Interesting properties…Reynolds Number (flow)
Interesting properties…Reynolds Number (flow)
Re = L Vavg ρ/μ
Interesting properties…Reynolds Number (flow)
Re = L Vavg ρ/μ▪ L = most relevant length
Interesting properties…Reynolds Number (flow)
Re = L Vavg ρ/μ▪ L = most relevant length▪ ρ = fluid density
Interesting properties…Reynolds Number (flow)
Re = L Vavg ρ/μ▪ L = most relevant length▪ ρ = fluid density▪ μ = viscosity
Interesting properties…Reynolds Number (flow)
Re = L Vavg ρ/μ▪ L = most relevant length▪ ρ = fluid density▪ μ = viscosity
Re < 100
Interesting properties…Reynolds Number (flow)
Re = L Vavg ρ/μ▪ L = most relevant length▪ ρ = fluid density▪ μ = viscosity
Re < 100 Re > 2000
Types of Flow
Types of Flow Pressure Driven Flow
Types of Flow Pressure Driven Flow
http://faculty.washington.edu/yagerp/microfluidicstutorial/tutorialhome.htm
Types of Flow Electrokinetic Flow
Types of Flow Electrokinetic Flow
http://faculty.washington.edu/yagerp/microfluidicstutorial/tutorialhome.htm
What is it?
What is it? Programmable chip + microfluidics
chamber
What is it? Programmable chip + microfluidics
chamberWhy do we need it?
What is it? Programmable chip + microfluidics
chamberWhy do we need it?
Larger quantities
What is it? Programmable chip + microfluidics
chamberWhy do we need it?
Larger quantities Smaller objects
What is it? Programmable chip + microfluidics
chamberWhy do we need it?
Larger quantities Smaller objects
DNA, living cells, droplets of chemical compounds
But how does it work?
But how does it work?
DIELECTROPHORESIS (DEP)
But how does it work?
DIELECTROPHORESIS (DEP) Non-uniform field induced dipole
But how does it work?
DIELECTROPHORESIS (DEP) Non-uniform field induced dipole Apply local e-field
But how does it work?
DIELECTROPHORESIS (DEP) Non-uniform field induced dipole Apply local e-field Different dielectric constant force
FDEP = 2πεma3 CM(ω) Grad(Erms2)
FDEP = 2πεma3 CM(ω) Grad(Erms2)
Clausius-Mossoti factor
FDEP = 2πεma3 CM(ω) Grad(Erms2)
Clausius-Mossoti factor CM(ω) = Re [ (εp - εm) / (εp + 2 εm )]
FDEP = 2πεma3 CM(ω) Grad(Erms2)
Clausius-Mossoti factor CM(ω) = Re [ (εp - εm) / (εp + 2 εm )]▪ CM(ω) < 0 nDEP
FDEP = 2πεma3 CM(ω) Grad(Erms2)
Clausius-Mossoti factor CM(ω) = Re [ (εp - εm) / (εp + 2 εm )]▪ CM(ω) < 0 nDEP▪ CM(ω) > 0 pDEP
http://www.blazelabs.com/pics/emep.gif
Voltage…
Voltage…AC fields
Voltage…AC fields
No ion shielding
Voltage…AC fields
No ion shielding Less harmful
Voltage…AC fields
No ion shielding Less harmful
5V CMOS process
Voltage…AC fields
No ion shielding Less harmful
5V CMOS process Lower noise
Voltage…AC fields
No ion shielding Less harmful
5V CMOS process Lower noise Less heat
Yeast
Yeast
Yeast Programming of large numbers of cells
Yeast Programming of large numbers of cells Assembly of tissue
Mammalian cells work too!
Mammalian cells work too!
Rat alveolar macrophages
Mammalian cells work too!
Rat alveolar macrophages
Mammalian cells work too!
Rat alveolar macrophagesPresumably
human cells work too!
APPLICATIONS!
APPLICATIONS!
Programmable chemistry
APPLICATIONS!
Programmable chemistryTissue assembly
APPLICATIONS!
Programmable chemistryTissue assembly
Art?
APPLICATIONS!
Programmable chemistryTissue assembly
Art? … no one will be able to see it…
Microfluids
Microfluids
ARE
Microfluids
ARE
very useful when combined with an integrated circuit.