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CONTENT

NAME OF THE ORGANISATION

REPUBLIC OF TURKEY MINISTRY OF AGRICULTURE AND RURAL AFFAIRSADANA FOOD CONTROL LABORATORY

LOCATION OF THE ORGANISATION

Adress : Kprl mah. 01230 Yreir ADANAPhone : 0 322 344 19 19Fax : 0 322 344 17 67Web : 01kontollab.kkgm.gov.trE mail :[email protected]

THE BRIEF HISTORY OF INSTITUTIONAdana Provincal Control Laboratory Directorate was established in 1987. During institution, 3 rooms of the top

flor become active as the Directorate of Cotton Breeding Station at Kprl with manager, 4 engineers,2 technicians and2 administrative staff in agricultural campus. Initially , there is no laboratory unit therefore the administrative structure

provided without laboratory services . Then by doing internal arrangements in garden of Adana Vocational School for

Agriculture was made laboratory with prefabricated buildings. The laboratory chief of the Adana Animal DiseasesResearch Institude ,settled in Kurttepe, provide transfer of device and chemical substances to our directorate. Analysis offood and feed started 45 days after moving.

In 1991 the protocol signed between our directorate and the Directorate of Cotton Breeding Station.Revolving Fund Management Directorate of the ownership of the Cotton Breeding Station Adana Kprl neighborhoodof the district administration building (two flor) and the laboratory building Adana Provincial Control LaboratoryDirectorate have been transferred to the order only to be given the right to use. The two storied building located on theground used as a museum of agriculture department was transferred into the laboratory with renovations and regulations. In1992 , February, administrative section of the directorate and Physical Analysis Laboratory , Additive Analysis Laboratorywere moved to this building . In 1993, Chemical Analysis Laboratory moved to additional laboratory building.

In 2000, maintanance and repairing of administrative building of our directorate and air conditioning of allunits was provided. In 2001, Microbiology Laboratory ; in 2002 Physical Analysis Laboratory were restored. In 2003Additive Analysis Laboratory and Mycotoxin Laboratory was carried out with renovation and repairing. In 2004, 32 unitsof laboratory equipment has been allocated to our directorate under the MEDA Project.

In 2006, 2 Gas Chromotography (GC) and Gas Chromotography with a Mass Spectrophotometer (GC MS)were taken by the Govenorship of Adana Province Administration Directorate under the Monitoring of Drug Residues inFoods Agricultural Project.

Cotton Breeding Station owned by Directorate of Adana in 2007, the unused building of Breeding StationLaboratory has been transferred by a protocol to laboratory directorate with repairing and regulations building work upinto laboratory and has started to work for Sample Admission and Adjustment and Mineral Analysis Laboratory.

Our directorate is working by following all kinds of national and international developments. At the same timein order to ensure international acceptability and validity of the experiments performed on a product basis , national and /or international Standard methods accredited by TURKAK on 21.11.2007 has been working every year by expanding thescope.

The main service units:1- Sample Admission and Adjustment2-Physical Analysis Laboratory3-Additive Residue Analysis Laboratory4-Chemical Analysis Laboratory

5-Microbiology Laboratory6-Mineral Analysis Laboratory7-Biosafety Analysis Laboratory
mailto:[email protected]:[email protected]:[email protected]


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PURPOSE OF SUMMER PRACTICE

Purpose is understanding how theoretical knowledges are acted practically and having experience at application areas.Also it is gaining technical knowledge-talent, practical knowledge-talent additional to knowledge learning from university.

SCOPE OF SUMMER PRACTICE

1.Laboratory analyses2.Production scale


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MANAGER

ASSISTANTMANAGER

QUALITY CONTROLMANAGEMENT

INTERNAL RESEARCH UNIT

UNIT OF MEASUREMENT

UNCERTAINTY

CALIBRATION UNIT

TRAINING OFFICER

EXECUTIVE

FINANCIALWORK

UNIT OF EUROPEANUNION

SAMPLE ADMISSION AND ADJUSTMENT

PHYSICAL ANALYSIS LABORATORY

ADDITIVE AND RESIDUE ANALYSIS

CHEMICAL ANALYSIS LABORATORY

MINERAL ANALYSIS LABORATORY

BIOSAFETY ANALYSIS LABORATORY

MICROBIOLOGY ANALYSIS

CAPITAL STOCK

ACCOUNTING

INTERNAL

CONTROL


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ADMINISTRATOR

ASSISTANT ADMINISTRATOR

FOODENG.6

AGRICULTURALEN.16

CHEMICALENG.1

ENG. OFFISHERIES

2

CHEMIST1

BIOLOGIST

3

VETERINARY1

TECHNICIAN1

LAB.TECH.4

OFFICER2

WORKER14

TOTAL 53


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NOT ACCEPTABLE

RESOLVED

ACCEPTANCE OF SAMPLE AND ARRANGEMENT REPORT

Control of samples and documentsbrought

Control of sampleat laboratory

Admission and storage of sample

Document register

Registration of sample acceptencebook

Referring SAA

Registration and of sample tracking system

Manager approval

Wage determination andpayment

Delivering of sample to laboratory with sample delivery form

Transport of sample

Delivering to laboratory

Giving back to SAA

nforming customer

Resolve theproblem

Analyzing and storage under

apropriate conditions

Destruction of sample

Sampleremained

Delivering of sample requested 15 days storage

Giving backsample


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FUNCTION OF QUALITY MANAGEMENT UNIT AND STRUCTURAL FORM1. Quality Management Unit provides documantation of good laboratory applications for registration, instruction,research, development, method, analysis of results, to control of the devices about reliabilities, to follow calibration of thedevices, system, program and procedures.2. A laboratory manual which is includes quality system purpose and targets, function and responsibilities, layot,facility and equipments, required datas about staff is prepared by Quality Management Unit.

3. All documents have to be clear, reachable applicable. These document must reach the target staff members.4. Unit appoints persons who know evaluating the results of tests, purpose of the tests, methods of the tests andprocedures.5. Unit keeps a document about methods, procedures, results and purposes of anaylsis. Moreover, these documentsare updated bye unit.6. Unit offers new projects which is include improvement of staff to the Director. It prepares standard operating

procedures for quality management unit.7. Directly connected to the Ministry of laboratory directorates,by the Ministry creation of two deputy directors, ifdeemed appropriate, quality management unit,headed by deputy technical manager consists of at least three people.8. This person must have worked in the laboratory for at least five years. QualityManagement Unit which trained

people and quality management issues need to be trained as chefs in laboratory.9. The only laboratory assistant in the offices of the administration sees fit, and the above-mentioned features ofthe Quality Management Unit which consists of at least three people and responsible to the Director.Laboratory is managed by directors and staff. But they have rules, procedures and standards which is include methods andmanagement of analysis. Quality of standard is named as ISO/IEC 17025. ISO/IEC 17025 is the main standard used by

testing and calibration laboratories.Laboratories use ISO/IEC 17025 to implement a quality system aimed at improving their ability to consistently producevalid results. It is also the basis for accreditation from an Accreditation Body. Since the standard is about competence,accreditation is simply formal recognition of a demonstration of that competence.A prerequisite for a laboratory to become accredited is to have a documented quality management system. The usualcontents of the quality manual follow the outline of the ISO/IEC 17025 standard.

CONTENTS OF THE ISO/IEC 170251) Scope2) Normative references3) Terms and definitions4) Management Requirementsi) Organizationii) Managemet Systemsiii) Document Controliv) Review of requests, tenders, and contractsv) Subcontracting of tests and calibrationsvi) Purchasing services and suppliesvii) Service to the customer viii) Complaintsix) Control of noncomforming and/or calibration workx) Improvementxi) Corrective Actionxii) Preventive actionxiii) Control of records

xiv) Internal auditsxv) Management review5) Technical requirementsi) Generalii) Personneliii) Acommodation and enviromental conditionsiv) Test and calibrations methods and method validationsv) Equipmentvi) Measurement traceabilityvii) Samplingviii) Handling of test and calibration itemsix) Assuring the quality of test and calibratio resultsx) Reporting the results


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ISOLATION OF DNA FROM FOOD ANDFEED

A genetically modified organism (GMO) or genetically

engineered organism (GEO) is an organism whose genetic

material has been altered using genetic engineering

techniques. These techniques, generally known as

recombinant DNA technology, use DNA molecules from

different sources, which are combined into one molecule

to create a new set of genes. This DNA is then transferredinto an organism, giving it modified or novel genes.

Transgenic organisms, a subset of GMOs, are organisms

which have inserted DNA that originated in a different

species.In this analyss DNA exraction was applied by

using canola.

ekil 1 seed canola

PROCEDURE

Homogenized about 0.2 gr material with a commercialhomogenizer.Preheated lysis buffer to 65 immediately before use.

Transfered the resulting powder to a 2 ml tube and added 550 lysis

buffer CF.Mixed carefully, added 10 proteinase K and mix again.


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Incubated at 65 for 30 min. Afterwards,cetrifuge the mixed for 10 min

(>10.000xg) to pellet contaminants and cell debris.

Pipette 300 clear supernatant into a new 1.5 ml centrifuge tube.Added

300 buffer C4 and 200 ethanol.Vortexed the mixture for 30 sec.

Placed a GENESpin column into a new 2 ml centrifuge tube and pipette

750 mixture onto the column.Centrifuged for 1 min at

10,000xg.Discarded flow-through.

Pipette 400 buffer CQW onto the GENESpin column.Centrifuged for 1

min at 10,000xg.Discarded flow-through.

Pipette 700 buffer C5 onto the column.Centrifuged for 1 min at

10,000xg.Discarded flow-through.

Pipette another 200 buffer C5 onto the GENESpin column.Centrifuged

for 2 min at full speed (10-16,000xg) in order to remove buffer C5

completely.

Place the GENESpin column in a new 1.5 ml centrifuge tube.Pipette 100

elution buffer CE onto the membrane.Incubated for 5 min.Centrifuged for

1 min at full speed to collect the DNA.

PREPARATION OF THE PCR MIXES

MIX FOR PLANT GENE-PCR

COMPONENT VOLUME

Plant Gene Master Mix 18

Enzyme Solution 1

Internal Control 1

TOTAL VOLUME 20

1.Thawed the solutions and, for maximal recovery of components, brieflyspined vials in a microcentrifuge before opening.Mixed carefully but thoroughly bypipetting up and down.

2.In a 1.5 ml reaction tube, prepared the PCR Mix by adding the following

components in the order mentioned below,then mixed gently but thorougly bypipetting up and down.

3.Mixed carefully but thorougly by pipetting up and down.Did not vortex

Pipet 20 PCR was mixed in to each well


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For the samples of interest,added up to 5 sample DNA (f less than 5

added H2O to 5 )to a well

For the negative control,added 5 H2O

Fort he positive control, added 5 foodproof GMO Screening ControlTemplate

4.Sealed the plate accurately with an optical sealing foil

5.Placed the plate in a swing bucket cetrifuge and centrifuged at

1,500xg for 30 s.

RESULT; There were found enough DNA

DNA CONCENTRATION 5.86 ng/ , DNA was diluted with 0,2 for PCR


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MINERAL ANALYSIS LABORATORYMinerals are essential for a healthy life. They are required to live without the body functions not remain in a healthy

way. Minerals are inorganic substances the body can not make. There are many minerals that are vital to our health morethan 15. Mainly vitamins, minerals, vitamins work together to provide access to the region in the greatest need. Forminerals, vitamins work the same way. Cell protection and healthy teeth, bones, skin structure is important for. Minerals aswell as blood pressure, heart rhythm, muscle function, fluid balance in the body housing, breeding, and many more play an

important role in the function. Scientific studies have shown that the lack of mineral loss, and directly affects our health.Some manufacturers have resorted to tricks we had to get minerals from the outside so mneral analyss are appled n thslaboratory.

DETERMINE THE AMOUNT OF SELENIUM IN THE EGGMineral Selenium - Selenium Benefits and Functions: A powerful antioxidant, selenium strengthens the immune

system and reduces the risk of cancer. Protects the cells and slows the aging process. Tissue, increasing the flexibility andhelps to protect heart cells, supporting the heart and vascular health. Sperm production and viability, including in

particular, plays a role in reproductive health. Reduce the harmful effects of toxic substances in the body. Activities in theliver contributes to maintain a regular basis.Our aim is to determine amount of selenium in egg by using ICP-0ES machine in the mneral analyss laboratory.

MATERIALS AND CHEMICALS; sensitive scale,blender, Teflon tube for combustion, graduated flask, ashless filterpaper, 35 mm or 45 mm diameter glass funnel, test tubes, fume hood, microwave sample preparation unit, ICP-OESmachine HNO3 (%65), ultra distilled water, standard preparing solution.

PROCEDURE Mixed sample by using mixer and made it homogeneous Taken 1 gr homogeneous egg and put in to teflon tube for combustion

Added 5 ml HNO3 in to teflon tube for combustion,waited for 20 min on the fume hood After that time, tubes were placed in to microwave sample preparation unit This process was taken 1 hour and after this process,sample was taken for fume hood, opening the cover of the

pressure inside the tube is reduced balloon flask was transferred to the contents of containers cooled with the aid of a funnel Diluted with ultra distilled water Prepared sample was put in to test tube and placed ICP-OES mechine

RESULT;Amount of analyss(ppm)=Amount of Concentraton in to ICP-AXIAL X Diluted Factor /1000

SELENIUM = 0,623 ppm


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ADDITIVE AND RESIDUE ANALYSIS LABORATORY

Today, agricultural products, harmful insects, weeds and pathogenic organisms in order to maintain and increaseagricultural yield to chemical control has become an inevitable requirement. Pesticide-called pests by eliminating thesesubstances in food or food substances to increase production while providing significant levels of lead contamination.

Excessive use of pesticides by producers with higher-quality product could be more because of the consumption of large

amounts of medicine in question. After which levels of pesticide residues in foodstuffs in general are known to affect.However, close to the harvest period and during harvest as a result of the spraying of pesticide residues in increasingquantities. Therefore, high levels of pesticide residues in foodstuffs may exist from time to time. Therefore, the initial

phase of pesticides and chemical warfare method was seen as a savior in the health and environmental problems caused bythe later emerged. Today has reached levels of pesticide residues to human health threat. Therefore, determination of

pesticide residues in food is a lot of food raw materials and finished products has become imperative. Organic Phosphorusand nitrogen in our laboratory group with the group of chlorinated organic pesticide analysis is done.

PESTCDE N APPLE,CRUET,ONION,CUCUMBER

Pesticides are substances or mixture of substances intended for preventing, destroying, repelling or mitigating anypest.A pesticide may be a chemical substance, biological agent (such as a virus or bacterium), antimicrobial, disinfectantor device used against any pest. Pests include insects, plant pathogens, weeds, molluscs, birds, mammals, fish, nematodes(roundworms), and microbes that destroy property, spread disease or are a vector for disease or cause a nuisance. Although

there are benefits to the use of pesticides, there are also drawbacks, such as potential toxicity to humans and other animals.According to the Stockholm Convention on Persistent Organic Pollutants, 9 of the 12 most dangerous and persistentorganic chemicals are pesticides. Thus we observed pesticide in apple,cruet,onon,cucumber.

In this experiment, we used blender, micro pipette, vial, centrifuge, GC-MS,LC- MS/MS, plastic or teflon tubes ,sensitive scalesREAGENT; MgSO4 , PSA, SODIUM ACETATE, %1(%1 ACETIC ACID %99 ACETONITRILE)

PROCEDURE

Prepared plastic tubes by weighing 6 gr MgSO4 and 1,5 gr sodum acetat and 0,6 gr MgSO4 and 0,2 gr PSA

Grinded sample by usng blender and weighed 15gr sample

Added 15 ml %1 acetic acid soluton in to sample

Mixed with vortex for one mnute

Centrifuged at 5000 rpm turnover

Taken 4 ml soluton from above centrifuged soluton and put in to plastic test tube that contans 0,6 gr MgSO4and 0,2 gr PSA

Mixed with hand or vortex

Centifuged at 5000 rpm turnover

Taken soluton from tubes by usng mcropipette and put in to vials

Put in to GC-MS and LC-MS/MS

RESULT; SMALLER THAN REPORTED LIMIT


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