b i o i n f o r m a t i c s an intro
TRANSCRIPT
P. Paulsharma Chakravarthy
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Bioinformatics – an Introduction
P. Paulsharma Chakravarthy
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P. Paulsharma Chakravarthy
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“Bioinformatics addresses problems related to thestorage, retrieval and analysis of information about
biological structure, sequence and function.
- National Institute of Health
Definition
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Bioinformatics deals with
• Design and implementation of new algorithms and statistics which assess relationship among members of large data sets.
• Analysis and interpretation of various data types, which includes nucleotide and amino acid sequences and structure of protein.
• To develop computational tools and databases that enables efficient analysis, access and management of biologically significant information.
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Bioinformatics and Human Genome Project
• The Human Genome Project is a 13-year effort coordinated by the U.S. Department of Energy and the National Institutes of Health.
• The project budget was 3 billion and it was completed three years before the proposed time due to the technological advancements in biological data storage, management and analysis.
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Necessary Skill Sets
• Knowledge in Molecular Biology• Statistics• Mathematics (algorithm development)• Communicate biological problems to computer
scientists• Working knowledge in bioinformatics tools• Computer proficiency (windows/command line)• Programming Skills• Data administration
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Specialized fields
• Computational Biology• Genomics• Proteomics• Bioprogramming• Cheminformatics• Structural Biology• Systems Biology• Pharmacogenomics
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Applications
• Sequence analysis and similarity searches
• Protein structure prediction• Phylogenetics• Molecular docking
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Sequence Analysis and Similarity Searches
• Finding the (protein-coding) gene Sequence alignment Sequence Comparison and functional annotation Domain and pattern analysis
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Finding the (protein-coding) gene?
Protein
mRNA
DNA
transcription
translation
CCTGAGCCAACTATTGATGAA
PEPTIDE
CCUGAGCCAACUAUUGAUGAA
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Pairwise Sequence Alignment
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Multiple Sequence Alignment
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Pfam analysis
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Structure Prediction
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Molecular Docking
