BACTERIALBASICS

Bacteria Structure

• Prokaryotes– No nucleus– Cell wall– DNA plasmid

(ring-shaped)

• Use asexual reproduction

http://en.wikipedia.org/wiki/Bacteria

Different Forms of DNA Transfer

Bacterial Transformation

• 1928-Frederick Griffith, 1944-Avery, McCarty, Macleod

• Process of taking up DNA from media

• DNA can be from cells

from same or different

species

• DNA will integrate into

the recipient’s

chromosome

http://en.wikipedia.org/wiki/Bacterial_transformation

Bacterial Adhesion Zones

• Adhesion zones develop in the bacteria cell membrane, although DNA cannot fit through

Calcium Chloride Solution

• The addition of calcium ions in CaCl2 solution shields the negatively charged phosphate groups so that the DNA can pass through the membrane when heat shocked.

Heat Shock 42ºC