bacterial culture mediaculture media
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BacterialBacterial Culture MediaCulture Media
C lt di tifi i ll Culture media : an artificially prepared substance containing prepared substance containing various microbial nutritional requirements at optimum pH which will allow the growth of which will allow the growth of most microorganisms. gIn order to cultivate these M.O. in large numbers to study them.
Types of media according to consistency:Types of media according to consistency:1. Liquid (broth)----- (0%) No agarq ( ) ( ) g2. Semi-solid ----- 0.5% agar3 S lid 2 3% 3. Solid ----- 2-3% agar
Solid media
Liquid media (Broth)
uninoculatedInoculated (turbid)
Use of broth in biochemical tests
B th diBroth media
+ve -ve
A-Simple media.(nutrient agar and broth, pepton water) B-Special media. 1. Enriched media. (blood, chocolate agar) ( , g )2. Selective media.(MacConkey, TCBS, SS and LJ)3. Differential media.(MacConkey, S-S, blood agar)3. Differential media.(MacConkey, S S, blood agar)4. Indicator media.(Mannitol Salt agar, MacConkey, EMB)EMB)5. Transport media.(Thioglycollate broth, Stuart’s medium)
Classification according to oxygenClassification according to oxygen requirement:
Aerobic media ( i l ti d di )-Aerobic media (previously mentioned media).
-Anaerobic media:Robertson’s cooked meat medium (RCM) Thioglycollate liquid mediumThioglycollate liquid medium.
Nutrient agarNutrient agarNutrient agarNutrient agarDifferent staphylococcal species on nutrient agar
Blood agar
Lactose fermenter Non-Lactose fermenter
Lactose Lactose fermenters as
l dpink colored colonies on MacConkey
mediummedium
Neutral red( i di )(as indicator)
Salmonella species Shigella
ChromagarChromagarChromagarChromagar
Vibrio cholerae
Mannitol Salt Agarg(Phenol red)
i l fMannitol fermenter
EMB (Eosin EMB (Eosin methylenemethylene blue) blue) ( t lli h l )( t lli h l )(green metallic sheen colony)(green metallic sheen colony)
Robertson’s cooked meat medium(RCM)