bd stem cell resource poster
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BD Stem Cell ResourceMarkers of Self-Renewal and Differentiation
23-9953-01
Ectoderm Markers Embryonic Stem Cells Mesoderm Markers Stem Cell Relevant SignalingAstrocytesA2B5CD44FGFR3
GFAPRan-2S100B
Embryonic Stem Cell MarkersAlkaline PhosphataseCD9CD15 (SSEA-1, Lewis X)CD24CD29 (Integrin β1)CD30CD49f (Integrin α6)CD133 (AC133, Prominin-1)CD194 (CCR4)CDw338 (ABCG2)c-MycCriptoDPPA4DPPA5 (ESG1)E-Cadherin (CD324)FGF-4FoxD3Frizzled-5GCNF (NR6A1)GCTM-2GDF-3Hsp27
Lefty-ALin-28Nanog (Human)Nanog (Mouse)Oct3/4PodocalyxinPRC2 (Suz12)Rex-1SFRP2Smad2/3Sox2SSEA-3SSEA-4STAT3STAT3 (pS727)STAT3 (pY705)STAT3-interacting protein 1TRA-1-60 AntigenTRA-1-81 AntigenUTF-1Zic3
Early Mesoderm MarkersBMP2BMP4BrachyuryCD31 (PECAM1)CD34 (Mucosialin, gp 105-120) Cryptic
MIXL1N-Cadherin (CD325)NF-YANodalSca-1 (Ly6A/E)Snail
Erk-MAPK Signalingbasic FGFC-RafERKERK1ERK1/2 (pT202/pY204)ERK2ERK3
Mek1Mek1 (pS298)Mek1/2 (pS222)Mek2p38 MAPK (PT180/pY182)Ras
TGF Beta SignalingBMPR-IICD119 (IFN-γ receptor α-chain)c-MycDP-1E2F-1E2F-2E2F-3Id1Id2
Id3p16 INK4p107p300PP2A Catalytic αROCK-IROCK-IISmad2/3SP1
Wnt Signaling14-3-3α-Cateninβ-Cateninδ-Cateninγ-CateninAktCaM Kinase IICasein Kinase I εCasein Kinase II αCasein Kinase II β
CBP (CREB-Binding Protein)GSK-3βGSK-3β (pY216)HDAC3Jagged1Neurogenin 3NicastrinNotch1PP2A Catalytic αWnt16
Jak-STAT SignalingCD130 (gp130)STAT1 (C-TERMINUS)STAT1 (pY701)STAT3STAT3 (pS727)STAT3 (pY705)
STAT4STAT4 (pY693)STAT5 (MGF)STAT5 (MGF) (PY694)STAT6STAT6 (pY641)
Notch SignalingCBP (CREB-Binding Protein)Jagged1Neurogenin 3
NicastrinNotch1
Heart - Cardiogenesis Markersα-ActininAnnexin VICardiac specific myosinCardiac Troponin ICardiac Troponin TCaveolin-2Caveolin-3CD31 (PECAM1)CD34 (Mucosialin, gp 105-120) CD66CD66cCD106 (VCAM-1)CD117 (SCF R, c-kit)CD144 (VE-cadherin)CD166 (ALCAM)
CD202b (TIE2)CD202b (TIE2) (pY992)CD202b (TIE2) (pY1102)Connexin-43DesminFlk-1 (KDR, VEGF-R2, Ly-73)GATA4GATA6HAND1HAND2M-CadherinMyogeninN-Cadherin (CD325)NF-YANkx2.5
Hemangioblast MarkersACEBracyuryCD31 (PECAM1)CD34 (Mucosialin, gp 105-120) CD133 (AC133, Prominin-1)CD144 (VE-cadherin)CD202b (TIE2)CD202b (TIE2) (pY992)CD202b (TIE2) (pY1102)
E-Cadherin (CD324)EphB4Flk-1 (KDR, VEGF-R2, Ly-73)GATA1LMO-2PodocalyxinRunx1 (CBFA2)Tal (SCL)
Hematopoietic Stem Cell MarkersNegative Markers
CD2CD3 CD4CD10CD13CD14CD15 (SSEA-1, Lewis X)CD15s (Sialyl Lewis x)CD16CD19CD20CD24
CD33CD36CD38CD45 (Leukocyte Common
Antigen, Ly-5)CD45R (B220)CD48CD56 (NCAM)CD97CD114 (G-CSF Receptor)CD138 (Syndecan-1) GATA3
Mesenchymal Stem Cell Differentiation MarkersChondrocyte (Cartilage) Markers
AggrecanCD44CD151Collagen II
Matrillin-4OsteonectinSox-9
Mesenchymal Stem Cell Markers (Fat)Negative Markers
CD11b (Integrin αM chain)CD14CD19CD34 (Mucosialin, gp 105-120)
CD45 (Leukocyte Common Antigen, Ly-5)
CD79aCD117 (SCF R, c-kit)HLA-DR
Positive Markers
CD9CD10CD13CD29 (Integrin β1)CD44CD49d (Integrin α4)CD49e (Integrin α5)CD54 (ICAM-1)CD55CD59
CD73 (Ecto-5’-nucleotidase)CD90 (Thy-1)CD105 (Endoglin)CD106 (VCAM-1)CD146 (MCAM, MUC18)CD166 (ALCAM)FibronectinHLA IVimentin
Skeletal Muscle Markersα-ActininActinB-Enolase (ENO-3)CD146 (MCAM, MUC18)DesminM-CadherinMyf5
MyoDMyogeninMyosin heavy chainMyosin light chainN-Cadherin (CD325)TitinTroponin
Smooth Muscle MarkersCalmodulinDesmin
Smooth muscle myosinVimentin
Mesenchymal Stem Cell Markers (Bone Marrow)Negative Markers
CD11a (Integrin αL chain)CD11b (Integrin αM chain)CD14CD19CD31 (PECAM1)
CD34 (Mucosialin, gp 105-120) CD45 (Leukocyte Common
Antigen, Ly-5)CD79aHLA-DR
Positive Markers
CD10CD13CD14CD15 (SSEA-1, Lewis X)CD29 (Integrin β1)CD44H (Pgp-1, H-CAM)CD49d (Integrin α4)CD49e (Integrin α5)CD51 (Integrin αV)CD54 (ICAM-1)CD56 (NCAM)CD71 (Transferrin Receptor)CD73 (Ecto-5’-nucleotidase)CD90 (Thy-1)
CD105 (Endoglin)CD106 (VCAM-1)CD117 (SCF R, c-kit)CD120a (TNF Receptor Type I)CD120b (TNF Receptor Type II)CD166 (ALCAM)CD271 (p75, NGFR/NTR)Flk-1 (KDR, VEGF-R2, Ly-73)Ly-6A/E (Sca-1)NucleosteminSTRO-1TAZVimentin
Myoblast (Muscle) Markers
AcetylcholinesteraseActinB-Enolase (ENO-3)CD146 (MCAM, MUC18)DesminLactate Dehydrogenase (LDH)
Myf-5MyoDN-Cadherin (CD325)Pax3Pax7PK-M
Osteoblast (Bone) Markers
Alkaline PhosphataseCollagen 1Fibronectin
IGFBP-3OsteocalcinRUNX2
Positive Markers
Bmi-1CaM Kinase IVCD31 (PECAM1)CD34 (Mucosialin, gp 105-120) CD44CD59CD90 (Thy-1)CD90.1 (Thy-1.1)CD105 (Endoglin)CD106 (VCAM-1)CD117 (SCF R, c-kit)CD133 (AC133, Prominin-1)CD164CD184 (CXCR4)CD201 (EPCR)CD202b (TIE2)CD202b (TIE2) (pY992)CD202b (TIE2) (pY1102)CDw93 (C1qRp)
CDw338 (ABCG2)DAZLFlk-1 (KDR, VEGF-R2, Ly-73)FOXO1G-CSF Gfi-1HOXB4Jak2Lmo2Ly-6A/E (Sca-1)MRP1N-Cadherin (CD325)NF-YANotch1P-glycoproteinRunx1 (CBFA2)Tal (SCL)WASP (Wiskott-Aldrich
Syndrome Protein)
Endothelial Cell MarkersCD14CD15 (SSEA-1, Lewis X)CD18 (Integrin β2 chain, CR3/CR4) CD29 (Integrin β1)CD31 (PECAM1)CD34 (Mucosialin, gp 105-120) CD36CD45 (Leukocyte Common
Antigen, Ly-5)CD45R (B220)CD49d (Integrin α4)CD49e (Integrin α5)CD49f (Integrin α6)CD54 (ICAM-1)CD56 (NCAM)CD62E (E-Selectin)CD62L (L-Selectin)CD62P (P-Selectin)CD102CD105 (Endoglin)CD106 (VCAM-1)CD109CD112CD116 (GM-CSF Receptor)CD117 (SCF R, c-kit)CD120a (TNF Receptor Type I)CD120b (TNF Receptor Type II)CD121a (IL-1 Receptor, Type I/p80)CD124 (IL-4 Receptor α)CD141 (Thrombomodulin)CD144 (VE-cadherin)CD146 (MCAM, MUC18)CD147 (Neurothelin)
CD151CD152 (CTLA-4)CD157CD166 (ALCAM)CD184 (CXCR4)CD192 (CCR2)CD201 (EPCR)CD202b (TIE2)CD202b (TIE2) (pY992)CD202b (TIE2) (pY1102)CD209CD209a (CIRE, DC-SIGN)CD213a1CD213a2CD249CD252 (OX-40 Ligand)CD253 (TRAIL) CD262 (TRAIL-R2, DR5)CD322CDw93 (C1qRp)CDw145Flk-1 (KDR, VEGF-R2, Ly-73)HIF-1αIP-10Ly-6A/E (Sca-1)N-Cadherin (CD325)NestinSTAT3STAT3 (pS727)STAT3 (pY705)STAT3-interacting protein 1Vimentin
Endoderm MarkersDefinitive Endoderm Markersα-FetoproteinB-CateninCD184 (CXCR4)GATA4
HNF-1β (TCF-2)HNF-3β (FoxA2)N-Cadherin (CD325)SOX-17
Hepatic Endoderm Markersα-FetoproteinAlbuminCD29 (Integrin β1)CD44H (Pgp-1, H-CAM)CD49f (Integrin α6)
CD90 (Thy-1)HNF-1αHNF-1β (TCF-2)Tat-SF1
Pancreatic Endoderm MarkersCD49f (Integrin α6)Gad65GlucagonGlut2InsulinIslet-1Islet-2NeuroD1
Neurogenin 3Neuropilin-2Pancreatic polypeptidePax-6PDX-1SomatostatinSynaptophysin
Primitive Gut Tube MarkersCDX2 HNF-1β (TCF-2)
Induced Pluripotency Markersc-MycKLF4Lin28
NanogOct3/4Sox2
Primordial Germ Cell MarkersBlimp-1CD15 (SSEA-1, Lewis X)CD117 (SCF R, c-kit)DAZLFABP9Fragilis
NanosOct3/4Piwi/HiwiSteelStella (Dppa3)VASA (DDX4)
Trophoblast MarkersCDX2Cytokeratin 7
Trop-2
Early Ectoderm MarkersNestinNotch1
TP63
Neural Crest Cell MarkersCD49d (Integrin α4)CD57 (HNK-1)CD271 (p75, NGFR/NTR)
MASH1Neurogenin 3Notch1
Neural Stem Cell MarkersA2B5CD15 (SSEA-1, Lewis X)CD15s (Sialyl Lewis x)CD24CD29 (Integrin β1)CD49f (Integrin α6)CD54 (ICAM-1)CD81 (TAPA-1)CD95 (Fas/APO-1)CD133 (AC133, Prominin-1)CD146 (MCAM, MUC18)CD184 (CXCR4)CD271 (p75, NGFR/NTR)CD349 (Frizzled-9)
CDw338 (ABCG2)Ki-67MSI1 (Musashi-1)MSl2 (Musashi-2)Nanog (Mouse)NestinNogginNotch1Pax-6Prominin-2PSA-NCAMSox1Sox2Vimentin
Neuronal Markersα-Synucleinα-Synuclein (pY125)CD24CD56 (NCAM)CD81 (TAPA-1)CD90 (Thy-1)CD90.1 (Thy-1.1)CD90.2 (Thy-1.2)CD112CD271 (p75, NGFR/NTR)ChATContactinDoublecortinGABA B Receptor Gad65GAP-43 (Neuromodulin)GluR δ 2GluR2GluR5/6/7Glutamine SynthetaseJagged1MAP2 (a+b)MAP2BMASH1
mGluR1mGluR1αN-Cadherin (CD325)NestinNeurofilament NF-MNeuron-restricted progenitorsNeuropilin-2Nicastrinp150 GluedPax-5P-glycoproteinPSD-95Serotonin Receptor 5-HT 2ARSerotonin Receptor 5-HT 2BRSMNSynapsin ISynaptophysinSynaptotagminSyntaxinTauTrkBTubbyTyrosine HydroxylaseVimentin
Oligodendrocyte MarkersCD44CD44H (Pgp-1, H-CAM)CD140a (PDGFR α)Galactocerebroside CMBP
O1O4Olig1Olig2RIP
Skin Precursor Cell MarkersCRABP2FibronectinNestin
Sca-1 (Ly6A/E)Vimentin
White colored markers are available from BD Biosciences
bdbiosciences.com/stemcell
LIFR
Jak
Stat3C-myc Sox2 Oct4 Nanog Smads 2/3
Smads 175 MEK/ERK
p pp
-catenin
WntBMP4 bFGF
TGF- /Activin
LIF
JAK-STAT
Self Renewal
Wnt BMP-SMad Erk-MAPKTGF- /Activin
gp130
LiverSelf Renewing Pathway
Heart Skeletal Muscle Kidney Smooth Muscle
Endothelium
Hemangioblast
Proerythroblast
MegakaryocyteMyeloid Dendrtitic CellMonocyte
Monoblast Megakaryoblast
Skin and Hair
Glial Restricted Progenitor
Type 1 Astrocyte
Type 2 Astrocyte
Neuron
OligodendrocyteProgenitor
Oligodendrocyte
NeuronalRestrictedProgenitor
Neural CrestStem Cell
Neurons, GliaSmooth MuscleCardiac TissueChondrocytes
Osteocytes
MacrophageThrombocytes
(Platelets)
Myeloblast
Neutrophil BasophilEosinophil
NeutrophilicMyelocyte
BasophilicMyelocyte
EosinophilicMyelocyte
NeutrophilicBand Cell
BasophilicBand Cell
EosinophilicBand Cell
Erythroblast Progranulocyte
Normoblast
Reticulocyte
Erythrocyte(Red Blood Cell)
Egg
Primordial Germ Cell
Sperm
Osteoblast (Bone)
Adipocyte (Fat)Fibroblast
Myotube (Muscle) Plasmacytoid
Dendritic CellCommitted Lymphoid Progenitor
Pre B CellPre-NK Cell
NK Lymphoblast
NK Cell
B-Lymphoblast
B Cell
Plasma Cell
T Cell
T-Lymphoblast
Thymocyte
MyoblastChondrocyte (Cartilage)
Thymus Pancreas Thyroid Lung Intestine
Endoderm
Mesenchymal Stem Cell
Hematopoietic Stem Cell
Mesoderm
Neural Stem Cell Ectoderm Embryonic Stem Cell or Induced Pluripotent
Stem Cell