betula uber
TRANSCRIPT
8/3/2019 betula uber
http://slidepdf.com/reader/full/betula-uber 1/17
JORESSIA A. JAMISON AND MICHAEL H.RENFROE
Department of Biology, JamesMadison University, Harrisonburg,
Virginia 22807
(Received 21 March 1997; accepted23 January 1998; editor T. A. Thorpe)
MICROPROPAGATIONOF BETULA UBER
MikropropagacijaBetule uber
8/3/2019 betula uber
http://slidepdf.com/reader/full/betula-uber 2/17
Virginijskaokroglolistna
breza
8/3/2019 betula uber
http://slidepdf.com/reader/full/betula-uber 3/17
GOJIE
Package SizePrice
($USD)
1L $1.25
10L $8.2550L $27.00
8/3/2019 betula uber
http://slidepdf.com/reader/full/betula-uber 4/17
+ hormoni (citikinini)
+
Autoklaviranje121°C1,05kg/cm215 min
8 g/L DifcoBocto agar
1M 5M 10 M
- 6 benzilamino adenin (BA) ali
- 2-izopentenil adenin (2iP) ali
- kinetn
8/3/2019 betula uber
http://slidepdf.com/reader/full/betula-uber 5/17
IZSEEK 4 - 6 SADIK(14,5 ± 64 cm)
Pomlad, poletje,
Mlado drevo,dobro razvejano
Sterilizacija
(15% Clorox 15 min)
7× destilirana voda
Nodije obreemo
-pokodovano tkivo
Kozarki
(babyfood jars)
Petrijevka
SEGMETI z
3 ± 4 nodiji
Spiranje izseka
Tween 20 (5 min)
8/3/2019 betula uber
http://slidepdf.com/reader/full/betula-uber 6/17
RASTN E KOMO R E
Dnevni cikel
28°C
15 ur (6:00-21:00)
Noni cikel
18°C
9h (21:00-6:00)
8/3/2019 betula uber
http://slidepdf.com/reader/full/betula-uber 7/17
BUD BREAK
8/3/2019 betula uber
http://slidepdf.com/reader/full/betula-uber 8/17
FIG. 1. Comparison of bud break of Betula uber
nodal explants over timestimulated by 2-iP (A), kinetin (B), and BA ((2) atdifferent concentrations.Each point represents the results from 20 cultureswith one explant per culture.
8/3/2019 betula uber
http://slidepdf.com/reader/full/betula-uber 9/17
FIG. 2. Comparison of mean shoot elongationof Betula uber nodal explantsat 4 wk stimulated by BA, 2-iP, and kinetin atdifferent concentrations.Each point represents the results from 20cultures with one explant per culture.
Basalcallusing ±bazalni kalus34% najve
Vitrifikacije NI
Temno zelena rastlina
8/3/2019 betula uber
http://slidepdf.com/reader/full/betula-uber 10/17
FIG. 3. Comparison of Betula uber bud break over time stimulated by BAat different concentrations, or BA in combination with GA 3. Each point
representsthe results from 20 cultures with one explant per culture.
FIG. 4. Comparison of mean shoot elongationof Betula uber nodal explantsover time stimulated by BA at differentconcentrations, or BA in combinationwith GA 3. Each point represents the resultsfrom 20 cultures withone explant per culture.
Preverjanje konc. Okrog 10M BA (7,5 M 10 M 12,5 M)+ 2,9 M GA
WPM + BA so neznatnomanj brstele (4 tedni)
WPM + BA in GA dobro
brstenje
1,9 cm
0,325cm
WPM + BA + GA
- bazalni kalus (44%)
- stekleni listi
WPM + BA
- ni steklenih listov
8/3/2019 betula uber
http://slidepdf.com/reader/full/betula-uber 11/17
POGANJEK 1,5 CM
P R ESA J AN JE I N KO R EN I N JEN JE
ZEMLJA
Promix/perlite
WPM + BA + GAslabo preneslepresajanje
WPM + BA
Dobro preivelepresajanje
77,8%
Najbolje ukoreninjenje WPM + 12,5 M BAGojie brez hormonov neuspenokoreninjenje
8/3/2019 betula uber
http://slidepdf.com/reader/full/betula-uber 12/17
RAZ I S K AV E / ANAL I Z E
8/3/2019 betula uber
http://slidepdf.com/reader/full/betula-uber 13/17
RAZ I S K AV E / ANAL I Z E
Polisadnimezofil(parenhim)
Debelinapovrhnjice intrihomi
Gostota in velikostlistnih re
SEM in vrstini elektronski mikroskop
8/3/2019 betula uber
http://slidepdf.com/reader/full/betula-uber 14/17
RAZ I S K AV E / ANAL I Z E
- po 1 tednu v in vitro
- po fazi prenosa
- v zemlji ( 2. teden)
- po aklimatizaciji
8/3/2019 betula uber
http://slidepdf.com/reader/full/betula-uber 15/17
8/3/2019 betula uber
http://slidepdf.com/reader/full/betula-uber 16/17
FENOTIPSKESPREMEMBE
8/3/2019 betula uber
http://slidepdf.com/reader/full/betula-uber 17/17
S K L E P I
Mona mikropropagacija Butule uber Gojie WPM z BA in GA nam daje najhitreje brstenje
Betule uber ± giberelinsko odzivna vrsta Aklimatizirane rastline imajo debelejo kutikolo Na podlagi re smo ugotovili FENOTIPSKI ODZIV kiomogoi preivetje rastlin glede na razmere
Te tehnike se lahko izkoristi za uporabo propagacije ostalihlistavcev ki so e ogroeni poleg Betule uber