Bhavin S. Bhatt

Research Scholar (Ph. D.)School of Life SciencesCentral University of Gujarat, GandhinagarGujarat – IndiaE mail: bhavin18@gmail.comMobile No. : +91 – 9825105890, +91-9408958748

Education

Degree Institution Specialization Year

Ph. D. School of Life Sciences, CentralUniversity of Gujarat, Gandhinagar

Life Sciences (PlantVirology)

2010 - pursuing(Thesis submitted,

May 2015)

M. Sc. University of Mysore, Mysore Biotechnology 2006

B. Sc. Sardar Patel University, VallabhVidyanagar

Biotechnology 2004

Research Projects:

Clonal propagation of Aloe barbadensis Mill.: An important medicinal plant.o Independent project performed under direction. The project entailed clonal propagation of

important medicinal plant, Aloe barbadensis Mill. Via apical meristem culture and in vitro

derived leaf base callus culture.

o Invitro plant propagation via apical meristem culture often use to produce disease free stock

of the plant & via callus culture use to induce somaclonal variation.

o Observation was made on effect of different kinds of growth hormones on response of

isolated plant tissues under in vitro condition and comparison of plant regeneration rate

propagated via apical meristem culture and callus culture.

o Plant tissue culture techniques, Good laboratory practices and aseptic conditions were used

considerably.

Isolation, Characterization and Antibiotic profiling of Salmonella typhi from Gujaratregion.

o The project was aimed at isolation and characterisation of Salmonella typhi from different

parts of Gujarat region in pure culture.

o Antibiotic activity and chromosomal DNA based or plasmid DNA based resistance to

antibiotic, commonly used to treat Salmonella infection, was checked.

o Results obtained were significant. Microbiology techniques, plating techniques and

maintenance of aseptic condition were used considerably.

Prospecting of genes for oil biosynthesis and storage in Jatropha curcas L. -Sponsoredby Department of Biotechnology, Ministry of Science and Technology, Govt. of India.As a Junior Research Fellow (JRF) at Plant Cell and Molecular Biology Department,Indian Institute of Advanced Research, Gandhinagar, Gujarat.

o Screening of the available germplasm for different stages of oil biosynthesis andaccumulation.

o Functional analysis of expressed sequence tags (ESTs) from the seeds of Jatropha.o Isolation of full length gene(s) of the fatty acid synthesis pathway in Jatropha.

Student member - Molecular characterization of begomoviruses infecting bitter gourd(Momordica charantia L.)-Sponsored by GUJCOST, Govt. of Gujarat. (Student Sci –Tech Project Scheme (Sanction Letter No.: GUJCOST/SSP/201666/2010-11/173).

o Survey and collection of Bitter gourd Leaf distortion Virus (BgLDV) infected bitter

gourd plant samples from Gujarat.

o Isolation and cloning of viral specific replicative DNA from infected plant samples.

o Sequencing and identification of viral genome.

o Development of PCR based diagnostic kits.

Currently Working: As a Research Scholar (Ph. D.) at School of Life Sciences, CentralUniversity of Gujarat, Gandhinagar.Ph. D. Thesis Title: “Molecular diversity and interactions of begomoviruses infectingFrench bean (Phaseolus vulgaris L.)” - thesis submitted to Central University of Gujarat,May 2015.

o Distribution and molecular diversity of bean infecting begomovirus from India.

o Construction of infectious clone/s of the viral DNA for Agrobacterium mediated

transformation.

o Differential pathogenicity among the begomovirus species infecting French bean.

o To study interaction between Bean infecting begomoviruses with other begomoviruses.

Career

2009 – 2010: Lecturer, Shree Ramkrishna Inst. of Computer Education and AppliedSciences, Surat.

2008 – 2009: Junior Research Fellow, Plant Cell and Molecular Biology Division,IndianInstitute of Advanced Research (IIAR), Gandhinagar.

2007 – 2008: Lecturer, Ashok and Rita Patel Institute of integrated study and research inBiotechnology Applied Sciences (ARIBAS), New V. V. Nagar.

2006 – 2007: Lecturer, Indu College of Medical Sciences, Vadodara.

Research interest

Viral diversity, Molecular biology of plant-virus interactions, genetically engineered viralresistance in plants, analysis of genes of plant viruses and the use of viral vectors for genesilencing in plants. Molecular basis of host pathogen interactions, plant genome evolution

Awards and honors

3rd rank in the M.Sc. Biotechnology examination, University of Mysore, Mysore. Joint CSIR – UGC test for Eligibility of Lecturership (Life Sciences), CSIR, New Delhi. National Eligibility Test (NET) for Lecturership (Environmental Sciences), UGC, New

Delhi. Best Paper Award in the International Conference on Advances in Biological Sciences

held at Kannur University, Kannur, Kerala, India March 15-17 2012. National Scholarship Programme, offered by Slovak Republic, Slovakia, February 2014 –

April 2014. National Eligibility Test (NET) for Lecturership (Agricultural Biotechnology), ASRB,

New Delhi. Prof. M. J. Narsimhan Academic Merit Award for best paper entitled “Analysis of

proteomic profile of French bean (Phaseolus vulgaris L.) upon infection withbegomovirus” by Indian Phytopathological Society, New Delhi (West Zone).

Publications

Vadodaria HK, Bhatt BS, Vaidya RP (2007). Clonal Propagation of Aloe BarbadensisMill.: An important medicinal plant via Callus culture and plant regeneration.International Journal of “Bioscience Reporter” (Currently Bioscience Guardian), 5(1),293-300. ISSN No. 2277-9493.

Bhatt BS, Vadodaria HK, Vaidya RP (2007). Clonal Propagation of Aloe BarbadensisMill.: An important medicinal plant via apical meristem culture. Journal of PhytologicalResearch, 20(1), 123-7. ISSN No. 0970-5767.

Bhatt BS, Vadodaria HK, Pandhi NH (2007). Isolation, Characterization and Antibioticprofiling of Salmonella typhi isolates from Gujarat (India) region. Int Journal of“Bioscience Reporter” (Currently Bioscience Guardian), 5(1), 331-6. ISSN No. 2277-9493.

Jain N, Bhargava P, Bhatt B, Chauhan H, Khurana P, Nadgauda R S (2009). ESTs fromJatropha curcas L. Seed cDNA Lidrary. ESTs sequence submission at NCBI database.

Rathore S, Bhatt BS, Yadav BK, Kale RK and Singh AK (2014). A new begomovirusspecies in association with betasatellite causing tomato leaf curl disease in Gandhinagar,India. Plant Disease. 98(3), 428. doi: 10.1094/PDIS-07-13-0719-PDN. ISSN: 0191-2917(*Contributed equally).

Bhatt BS, Rathore S and Singh AK (2015) Dwarf mosaic disease of French bean in Indiacaused by Rhynchosia yellow mosaic virus in association with a betasatellite. PlantDisease. Accepted for publication. doi: 10.1094/PDIS-02-15-0201-PDN. ISSN: 0191-2917.

George B, Bhatt BS, George B, Awasthi M and Singh AK (2015). Comparative analysisof simple sequence repeats in chloroplast genomes of lower and higher plants. CurrentGenetics. doi: 10.1007/s00294-015-0495-9. ISSN: 0172-8083.

Bhatt BS, Chahwala F, Rathore S and Singh AK (2015) Identification and molecularcharacterization of new recombinant begomovirus and associated betasatellite DNAinfecting Capsicum annum in India. Archives of Virology. Submitted

Referred papers in conference proceedings (Oral/Poster Presentations)

Poster presentation entitled “Isolation, Characterization and Antibiotic Profiling ofSalmonella typhi from Gujarat region” at AMI 47th annual conference-2007, organized byBarkatullah University, Bhopal (December 6 – 8, 2006).

Poster presentation entitled “Clonal propagation of Aloe barbadensis Mill.: An importantmedicinal plant via apical meristem culture” at XXI Gujarat Science Congress, organizedby Hemchandracharya North Gujarat University, Patan (March 11, 2007).

Poster presentation entitled “Molecular characterization of recombinant begomovirus andbetasatellite causing leaf curl disease of okra in India” at “International conference onAdvances in Biological Sciences” organized by Dept. of Biotechnology andMicrobiology, Kannur University, Kannur, Kerala, India (March 15 – 17, 2012) - Bestposter presentation award (IInd)

Poster presentation entitled “Molecular diversity and genome organization ofbegomovirus infecting Guar (Cyamopsis tetragonoloba L.) Taub. from Gandhinagar,Gujarat, India” at International conference on advances in Biotechnology andBioinformatics” organized by Dr. D. Y. Patil Vidyapith, Pune (November 25 – 27, 2013).

Oral presentation entitled “Molecular characterization of begomovirus causing dwarfmosaic disease of French bean (Phaseolus vulgaris L.) in India” at Asia Pacific Congressof virology(Virocon 2013) organized by Amity institute of Virology and Immunology,Amity University, Noida (December 17 – 20, 2013).

Oral presentation entitled “Molecular diversity and phylogeography of Begomovirusesinfecting vegetable crops in India” at Advances in Plant breeding and Biotechnologytechniques organized by Doctoral School of The Faculty of Agricultural and FoodSciences University of West Hungary, Mosonmagyaróvár, Hungary (April 28 – 29,2014).

Oral presentation entitled “Legume yellow mosaic viruses: A prolific threat to grainlegume production in India” at Modern trends in biodiversity conservation and itssustainable utilization (NCBCSU 2014) organized by Dept. of Botany, Periyar ArtsCollege, Cuddalore (July 17 – 18, 2014).

Oral presentation entitled “Analysis of proteomic profile of French bean (Phaseolusvulgaris L.) upon infection with begomovirus” at Plant Pathology: disease diagnosis andmanagement for sustainable agriculture organized by Indian Phytopathological society,New Delhi (West zone) and Maharastra Maghavidyalaya, Nilanga (January 24 – 25,2015). 1st rank in Prof. M.J. Narsimhan academic merit award contest (IPS, New Delhi,West Zone) - Best oral presentation award - Ist.

Oral presentation entitled “Proteomic approaches to study Plant – Virus interactions” atInternational seminar on Frontiers in Life Sciences Research: Challenges and Prospects

(BiYoM – 2015) organized by School of Life Sciences, Central University of Gujarat,Gandhinagar (February 23, 2015).

Oral presentation and Prof. M.J. Narsimhan academic merit award contest (National)entitled “Analysis of proteomic profile of French bean (Phaseolus vulgarisL.) uponinfection with begomovirus” at national symposium on “Understanding host – pathogeninteraction through science of omics” at ICAR – Indian Institute of Spices Research,Kozhicode, Kerala organized by Indian Phytopathological society, New Delhi and ICAR– Indian Institute of Spices Research, Kozhicode, Kerala (March 16 – 17, 2015).

Full length nucleotide sequences submitted to GenBank, NCBI (www.ncbi.nlm.gov)

Jain N, Bhargava P, Bhatt B, Chauhan H, Khurana P, Nadgauda R S (2009). ESTsfromJatropha curcas L. Seed cDNA Library. ESTs sequence submission at NCBIdatabase.

Bhatt B.S., Singh R.P., Kale R.K. and Singh A.K. (2012) Mungbean yellow mosaic IndiavirusClone-1 [India-Varanasi-Frenchbean01-2011] complete DNA-A. Submitted toNCBI GenBank accession no. KC019303.

Bhatt B.S., Singh R.P., Kale R.K. and Singh A.K. (2012). Mungbean yellow mosaicIndia virusClone-22 [India-Varanasi-Frenchbean02-2011] complete DNA-A. Submittedto NCBI GenBank accession no. KC019304.

Bhatt B.S., Singh R.P., Kale R.K. and Singh A.K. (2012). Mungbean yellow mosaicIndia virusClone-14 [India-Varanasi-Frenchbean01-2011] complete DNA-B. Submittedto NCBI GenBank accession no. KC019305.

Bhatt B.S., Singh R.P., Kale R.K. and Singh A.K. (2012). Horsegram yellow mosaicvirusclone-15 DNA-A complete sequence [India-Banglore-Frenchbean01-2011].Submitted to NCBI GenBank accession no. KC019306.

Bhatt B.S., Sangeeta and Singh A.K. (2012). Horsegram yellow mosaic virus clone-15 DNA-A complete sequence [India-Banglore01-Frenchbean02-2011]. Submitted toNCBI GenBank accession no. KP752088.

Bhatt B.S., Singh R.P., Kale R.K. and Singh A.K. (2012). Horsegram yellow mosaicvirusclone-15 DNA-B complete sequence [India-Banglore-Frenchbean01-2011].Submitted to NCBI GenBank accession no. KC019307.

Bhatt B.S., Sangeeta and Singh A.K. (2012). Horsegram yellow mosaic virus DNA-B complete sequence [India-Banglore-Frenchbean02-2011]. Submitted to NCBIGenBank accession no. KP752089.

Bhatt B.S., Yadav B.K., Sangeeta R., Singh R.P., Kale R.K. and Singh A.K. (2012).Okra enation leaf curl virus [India- Gandhinagar- 2012] clone-3 segment DNA-A, complete sequence. Submitted to NCBI GenBank accession no. KC019308.

Bhatt B.S., Yadav B.K., Sangeeta R., Singh R.P., Kale R.K. and Singh A.K. (2012).Okra enation leaf curl virus [India- Gandhinagar- 2012] clone-1 segment DNA-A, complete sequence. Submitted to NCBI GenBank accession no. KC019309.

Bhatt B.S., Yadav B.K., Sangeeta R., Singh R.P., Kale R.K. and Singh A.K. (2012).Okra enation leaf curl betasatellite [India- Gandhinagar- 2012] Clone-20 completesequence. Submitted to NCBI GenBank accession no. KC019310.

SangeetaRathore, Bhatt B.S., Yadav B.K., Kale R.K. and Singh A.K. (2012). Tomatoleaf curl Gandhinagar virus-[India-Gandhinagar-2012] complete DNA-A sequence

Submitted to NCBI GenBank accession no. KC952005. SangeetaRathore, Bhatt B.S., Yadav B.K., Kale R.K. and Singh A.K. (2012). Chillileaf

curl virus-[India-Anand-Tomato-2012] complete DNA-A sequence Submitted to NCBIGenBank accession no. KF515609.

SangeetaRathore, Bhatt B.S., Yadav B.K., Kale R.K. and Singh A.K. (2012). Tomatoleaf curl Gujarat virus-[India-Surat-2012] complete DNA-A sequence Submitted toNCBI GenBank accession no. KF515618.

SangeetaRathore, Bhatt B.S., Yadav B.K., Kale R.K. and Singh A.K. (2012). Tomatoleaf curl New Delhi virus-[India-Junagadh-2012] complete DNA-Asequence Submittedto NCBI GenBank accession no. KF515613.

SangeetaRathore, Bhatt B.S., Yadav B.K., Kale R.K. and Singh A.K. (2012). Tomatoleaf curl New Delhi virus-[India-Bhavnagar-2012] complete DNA-Asequence Submittedto NCBI GenBank accession no. KF515611.

SangeetaRathore, Bhatt B.S., Yadav B.K., Kale R.K. and Singh A.K. (2012). Tomatoleaf curl Gujarat virus-[India-Valsad-2012] complete DNA-A sequence Submitted toNCBI GenBank accession no. KF515616.

SangeetaRathore, Bhatt B.S., Yadav B.K., Kale R.K. and Singh A.K. (2012). Tomatoleaf curl New Delhi virus B-[India-Junagadh-2012] complete DNA-B sequenceSubmitted to NCBI GenBank accession no. KF515614.

SangeetaRathore, Bhatt B.S., Yadav B.K., Kale R.K. and Singh A.K. (2012). Tomatoleaf curl Gandhinagar betasatellite-[India-Gandhinagar-2012] complete sequenceSubmitted to NCBI GenBank accession no. KC952006.

SangeetaRathore, Bhatt B.S., Yadav B.K., Kale R.K. and Singh A.K. (2012). Tomatoleaf curl Karnataka betasatellite-[India-Anand-2012]complete sequence Submitted toNCBI GenBank accession no. KF515610.

SangeetaRathore, Bhatt B.S., Yadav B.K., Kale R.K. and Singh A.K. (2012). Tomatoyellow leaf curl Thailand betasatellite-[India-Surat-2012] complete sequence Submittedto NCBI GenBank accession no. KF515619.

SangeetaRathore, Bhatt B.S., Yadav B.K., Kale R.K. and Singh A.K. (2012). Tomatoleaf curl Karnataka betasatellite-[India-Bhavnagar-2012] complete sequence Submittedto NCBI GenBank accession no. KF515612.

SangeetaRathore, Bhatt B.S., Yadav B.K., Kale R.K. and Singh A.K. (2012). Tomatoleaf curl Karnataka betasatellite-[India-Junagadh-2012] complete sequence Submitted toNCBI GenBank accession no. KF515615.

SangeetaRathore, Bhatt B.S., Yadav B.K., Kale R.K. and Singh A.K. (2012). Tomatoyellow leaf curl Thailand betasatellite-[India-Valsad-2012] complete sequence Submittedto NCBI GenBank accession no. KF515617.

Yadav B.K., Bhatt B.S., Rathore S., Kale R.K. and Singh A.K. (2012). Okra leaf curlvirus-[India-Mau-Sal3-2012] complete DNA-A sequence submitted to NCBI GenBankaccession no. KF515621.

Yadav B.K., Bhatt B.S., Rathore S., Kale R.K.and Singh A.K.(2012). Okra leaf curlvirus-[India-Mau-Sac2-2012] complete D N A - A sequence submitted to NCBIGenBank accession no. KF515622.

Yadav B.K., Bhatt B.S., Rathore S., Kale R.K.and SinghA.K.(2012). Okra leaf curlbetasatellite-[India-Mau-2012] submitted to NCBI GenBank accession no.KF515623.

Bhatt B.S., Sangeeta and Singh A.K. (2015) Mungbean yellow mosaic India virus -[India-Jaunpur-Frenchbean-2011] complete DNA-A. Submitted to NCBI GenBankaccession no. KP779630.

Bhatt B.S., Sangeeta and Singh A.K. (2015) Mungbean yellow mosaic India virus -[India-Varanasi-Frenchbean02-2011] complete DNA-B. Submitted to NCBI GenBankaccession no. KP779631.

Bhatt B.S., Sangeeta and Singh A.K. (2015) Mungbean yellow mosaic India virus -[India-Jaunpur-Frenchbean-2011] complete DNA-B. Submitted to NCBI GenBankaccession no. KP779632.

Bhatt B.S., Sangeeta and Singh A.K. (2015) Mungbean yellow mosaic India virus -[India-Satna-Frenchbean-2012] complete DNA-A. Submitted to NCBI GenBankaccession no. KP779633.

Bhatt B.S., Sangeeta and Singh A.K. (2015) Mungbean yellow mosaic India virus -[India-Satna-Frenchbean-2012]. Submitted to NCBI GenBank accession no. KP779634.

Bhatt B.S., Sangeeta and Singh A.K. (2015) Mungbean yellow mosaic India virus -[India-Akola-Frenchbean-2014] complete DNA-A. Submitted to NCBI GenBankaccession no. KP779635.

Bhatt B.S., Sangeeta and Singh A.K. (2015) Mungbean yellow mosaic India virus -[India-Akola-Frenchbean-2014] complete DNA-B. Submitted to NCBI GenBankaccession no. KP779636.

Bhatt B.S., Sangeeta and Singh A.K. (2015) Rhynchosia yellow mosaic virus Clone 05[India-Gandhinagar-Frenchbean- 2014] complete DNA-A. Submitted to NCBI GenBankaccession no. KP752090.

Bhatt B.S., Sangeeta and Singh A.K. (2015) Rhynchosia yellow mosaic virus Clone 05[India-Gandhinagar-Frenchbean- 2014] complete DNA-B. Submitted to NCBI GenBankaccession no. KP752091.

Bhatt B.S., Sangeeta and Singh A.K. (2015). Corchorus yellow vein mosaic betasatellite-[India-Gandhinagar-Frenchbean- 2014] complete sequence Submitted to NCBIGenBank accession no. KP752092.

Academic achievements

I have been amongst top students of my batch at Masters’ course at the University ofMysore, Mysore (India). I secured third rank in the M.Sc. Biotechnology examination.

Other achievements

I have attended 15 Day annual camp of National Service Schemeprogramme at undergraduate level.

Worked as an editorial team member of abstract book / Souvenir preparation for “RecentAdvances in Cancer Research: Bench to Bedside” organized by School of LifeSciences, Central University of Gujarat, Gandhinagar. (February 19 – 20, 2011).

Member of Interim Student’s council. Central University of Gujarat (2011 – 12). Member of election committee, Student’s council, Central University of Gujarat (2012 –

13).

Member of election committee, Student’s council, Central University of Gujarat (2013 –14).

Worked as an editorial team member of abstract book / Souvenir preparation for“Frontiers in Life Sciences Research: Challenges and Prospects” organized bySchool of Life Sciences, Central University of Gujarat, Gandhinagar. (February 23,2015).

Personal Contact/Information:

Name Bhavin Sudhirkumar BhattFather’s Name Sudhirkumar C. BhattDate of Birth May 18, 1984Address 303, Pratik Apartments, B/H Kakadia

Complex,Nr. Parle Point, Ghod dod road,Surat- 395 007.Gujarat-India.

Phone No. M) +91-9825105890, +91-9408958748

(H) +91-261-2222618

Email Address bhavin18@gmail.comSex MaleMarital Status MarriedSpouse Name Dr. Meghana Bhavin BhattNationality IndianLanguages known English, Hindi, Gujarati

Bhavin S. Bhatt

References:

1) Dr. Achuit Kumar SinghAssistant Professor,School of Life SciencesCentral University of Gujarat,Gandhinagar Gujarat – IndiaE-mail: achuit.singh@cug.ac.inContact No. : +91 – 9409566489

2) Dr. Umesh Chand Singh YadavAssistant Professor,School of Life SciencesCentral University of Gujarat,Gandhinagar Gujarat – IndiaE-mail: umeshyadav@cug.ac.inContact No. : +91 – 79 -23260341

3) Prof. Rana P. SinghProfessor, School of Life SciencesJawaharlal Nehru University,New Delhi, India.Ex-Dean and Professor (Adjunct),School of Life SciencesCentral University of Gujarat,Gandhinagar Gujarat – IndiaE-mail: rana_singh@mail.jnu.ac.inContact No. : +91 – 11 -26704503

Detail Educational Records(Bhavin S. Bhatt)

QUALIFICATION YEAR INSTITUTE SUBJECTS % / GRADESS.S.C / Std 10th March 1999 Gujarat Secondary

Education Board, IndiaEnglish, Social Sciences, Math, Science, Gujarati

Extra Subjects taken: Sanskrit.86.86%

H.S.C / Std 12th April 2001 Gujarat SecondaryEducation Board, India

English, Math, Physics, Chemistry, Biology, Sanskrit. 66.15%(PCBM- 68.45%)

B. Sc. 1st year April 2002 N. V. Patel College ofPure and Applied

Sciences- Sardar PatelUniversity, Gujarat.

(India).

Physics, Chemistry Biology, English 64.27%B. Sc. 2nd year March 2003 Principle: Biotechnology.

Supporting: Biochemistry.Allied: Microbiology & Biology.

72.41%

B. Sc. 3rd yearBiotechnology

April 2004 Genetics & Molecular Biology, Recombinant Technology &Genetic engineering, Biomolecules & Metabolism,

Immunology & Virology, Environmental & MicrobialBiotechnology, Animal & Plant Biotechnology.

75%

M. Sc. Biotechnology June2006

University of Mysore,Mysore.

Biochemical techniques, Biophysics, Biochemistry,Microbiology, Molecular genetics, Cell biology, Molecular

biology, Genetic engineering, Computer application &Biostatistics, Immunology, Bioinformatics, Microbial

Technology, Food Biotechnology, Molecular Plant Pathology,Seed biology & Biotechnology and Cell and tissue culture

technology.

71.8%(3rd rank in university &

1st rank in college)

Ph. D. Life Sciences August 2010– Pursuing

Central University ofGujarat, Gandhinagar

Life Sciences (Plant Virology, Plant Molecular Biology) 8.5/10 CGPA (CourseWork)

National Eligibility Test(NET)

December2010

CSIR, New Delhi Life Sciences ---

National Eligibility Test(NET)

June 2012 UGC, New Delhi Environmental Sciences ---

National Eligibility Test(NET)

November2014

ASRB, New Delhi Agricultural Biotechnology ---

Bhavin S. BhattSchool of Life Sciences CentralUniversity of Gujarat Gandhinagar– 382030 Gujarat – India(M) +91-9825105890, +91 -9408958748bhavin18@gmail.com

I am Bhavin S. Bhatt, aspired student pursuing Ph.D. degree in Life Sciences at Central University of Gujarat,Gandhinagar. I take up the chance to introduce myself to apply for the prospective position at your reputedinstitute. With my educational background and working experience in the field of Plant Sciences, I stronglybelieve that I am suitable candidate for the position.

The adage “Necessity is the mother of invention” underlines the vital fact that all human endeavour is directedat fulfilling human needs. Every innovation holds its own importance. Keyed up by the idea of becoming ascientist since childhood, my interests in pursuing graduate studies intensified during my undergraduateeducation and research experience. I have completed Bachelor of Science and Masters of Science degree inBiotechnology in first class with distinction at Sardar Patel University, Vallabh Vidyanagar and University ofMysore, Mysore, respectively. Rather than dispiriting me with the tremendous amount of work and demandsfor creativity, my college years motivated me to pursue a doctorate degree. My interests include studying themolecular basis of host pathogen interactions for plant viral diseases.

Working in the laboratory, testing the hypothesis and analysing results are the key part of any research. Ilearned all basic techniques in Molecular Biology at my Junior Research Fellow (JRF) position at IndianInstitute of Advanced Research, Gandhinagar. Continuing and extending the knowledge, I am currentlycompiling for my doctorate thesis in the area of Plant Virology, supervised by Dr. Achuit Kumar Singh.Global increase in population with urbanisation demands more food to be supplied to evade malnutrition.Application of modern biology techniques into traditional farming practices is the best way to combatmalnutrition through increase yield of plant products.

For my Ph. D. dissertation, I worked upon assessing molecular diversity of begomoviruses infecting Frenchbean (Phaseolus vulgaris L.) in the Indian subcontinent. Cloning, Sequence analysis and glass houseinfectivity of viral clones and post analysis of infection process by SYBR based RT PCR techniques wereemployed to achieve the objectives.

It is amazing how erudite people can inspire you to stretch beyond your limits by virtue of their thoughtprovoking ideologies. During my doctoral dissertation, I availed National Scholarship offered by The SlovakRepublic. I have worked in the lab of Dr. Hajduch at Department of Reproduction and DevelopmentalBiology, Institute of Plant Genetics and Biotechnology to understand proteomic aspects of host pathogeninteraction and how plant responses upon the infection of pathogen by proteomic aspects.

I firmly believe that I will be an ideal candidate for the said position, which would help me to satisfy myenthusiasm for learning and contributing to the society.

I hope that having gone through my application; committee will reckon me suitable for position and grant methe chance to prove myself. I would like to conclude with one of my favourite quotes “To be well informed isnot a matter of knowing a little about everything, but of knowing everything about a little”.

Summary_Ph. D. Thesis Bhavin S. Bhatt

1 | P a g e

Title: Molecular diversity and interactions of begomoviruses infecting French bean

(Phaseolus vulgaris L.)

The present study reveals the identity and distribution of French bean infecting

begomoviruses in India. Dwarf mosaic disease and Yellow mosaic disease are two premier

causes for cultivation of, not only French bean, but major legume plants.

In the present study, a nationwide survey was carried to determine disease incidence on

French bean. Plants showing mosaic, motteling, stunted and dwarfism symptoms were

collected. Presence of begomoviral infection was confirmed by begomovirus degenerate

primer from French bean samples collected from six places out of total twelve places

surveyed. Begomovirus genomic components were cloned at respective cloning sites. RFLP

pattern showed that French bean samples collcected from each places are infected with two

types of virus molecules. Sequence analysis and genome organization of viral genome

revealed that three species of begomoviruses were predominantly present in these affected

areas of Indi which cause the disease in French bean crop. In Varanasi, Jaunpur, Satna and

Akola districts French bean plant were infected with MYMIV and identified as OW bipartite

begomovirus species. Whereas in bengaluru districts, a bipartite HgYMV causing dwarf

mosaic disease in French bean. In Gandhinagar district bipartite RhYMV in association with

CoYVMB causing dwarf mosaic disease in French bean. Partial tandem repeats of MYMIV

– Varanasi and MYMIV – Satna failed to induce visible symptoms on N. benthamiana, yet

measurable quantities of virus was found by RT - PCR. However, on inoculations of PTRs

of HgYMV – Bengaluru, RhYMV – Gandhinagar and RhYMV – Gandhinagar in

association with CoYVMB could able to induce systemic symptoms on N. benthamiana.

On French bean plants (P. vulgaris cv. Arka Komal) PTRs of MYMIV – Varanasi, MYMIV

– Satna, HgYMV – Bengaluru and RhYMV – Gandhinagar produce visible symptoms.

However, when co-inoculated with cognate DNA – B component, they express severe and

early symptoms on French bean plant as compared to inoculated alone. Also, RhYMV –

Gandhinagar (DNA – A and DNA – B) in association with CoYVMB produce much severe

symptoms that were alike filed symptoms observed on French bean.

Summary_Ph. D. Thesis Bhavin S. Bhatt

2 | P a g e

Absolute quantification of virus DNA was carried out through SyBr Green based RT – PCR.

Quantitative polymerase chain reaction results of suggest that associated DNA – B molecule

is absolutely essential for systemic movement and maintenance of helper virus inside plants.

Altogether, these results point out indispensable role of DNA – B molecule in ow bipartite

begomoviruses.

In Gandhinagar districts, a novel tripartite association (bipartite RhYMV+CoYVMB)

causing DMD on French bean crops. French bean plants co-inoculated with either DNA – B

or betasatellite produce symptoms but they were milder then symptoms observed upon

inoculation of all three components. Also level of helper virus was found significantly

higher in tripartite inoculations. These real time PCR experiments confirmed that associated

betasatellite is not only essential for symptom expression but also equally essential for

accumulation of helper viral DNA in a host plant.

Infectivity studies of MYMIV – Varanasi and HgYMV – Bengaluru were accessed on

tolerant variety of French bean cv. Arka Suvidha. Symptomatology and quantification

analysis revealed that symptoms expression and virus accumulation was lowered in tolerant

variety. In order to better understand the defense response induced in French bean, we

investigated the changes in the soluble proteome of French bean leaves infected by MYMIV

– Varanasi or HgYMV – Bengaluru using 2-DGE and MALDI-TOF-MS. A total of 40

proteins (20 each for MYMIV – Varanasi or HgYMV – Bengaluru) were identified that

showed changed in abundance. These proteins are indicated to be involved in multiple

biological processes, including primary and secondary metabolism, Energy provision, signal

transduction, defence and cellular homeostasis. Interestingly, among 20 proteins identified

for each infection, 13 proteins are common that showed differential expression upon

MYMIV – Varanasi or HgYMV – Bengaluru infection. These common proteins were shown

to involve in primary metabolism, cellular structure maintenance (Prohibitin), shielding of

host genome (NAP – 1), primary metabolism (OEEP1) and signal transduction (CBP). All of

this effort should lead to the understanding of the molecular basis of plant adaptation toward

the biotic stress. Such information can be than used for the development of broad spectrum

resistance strategies against diverse biotic stresses.

Summary_Ph. D. Thesis Bhavin S. Bhatt

3 | P a g e

Finally, we had looked for synergism, transcomplementation and trasreplication studies with

diverse betasatellites, major cause for recombination and evolution of new species. The

aetiology of symptoms and the capacity of the noncognate DNA – B or betasatellite not to

be replicated by helper begomovirus species lead us to further conclude that the

begomoviruses identified in this study are ow bipartite begomoviruse, where both DNA –A

and DNA – B has intimacy for each other for their co-existence. This may be a prime reason

for nonrecombinogenic nature of legumoviruses. But, as results obtained with tripartite

association of begomovirus in Gandhinagar sample, we cannot overlook that legumes may

not express symptoms but can be a best reservoir of diverse non leguminous betasatellite,

which led to possible recombination or formation of monopartite virus. as for instance,

occurance of recombinant French bean leaf curl virus along with French bean leaf curl

betasatellite infecting French bean plants in Kanpur, India.

In this present study identified and characterized three viruses MYMIV, HgYMV and

RhYMV from different regions of India. DMD or YMD of legumes has become one of

major limiting factor for production of French bean in India. This study provide base to

strategize further to develop long term stable resistant variety of French bean against virus

attack. Understanding host – pathogen interactions and mechanism of defence stratagems

could be important future aspect.