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BIMM 120 Winter 2009 TA: Abraham TangSection: Mondays 6PM; Thursdays 8AM [email protected]: by appointment

Week 1 material revisited

(bolded and underlined text indicates corrections or additional relevant

material not previously mentioned)1. Outgrowth of Kochs Discoveries

a. Criteria for establishing a causative link between an infectious

agent and a disease

b. Kochs postulates

i. The microbe must be found in all cases of the disease, but

absent from healthy individuals

ii. The microbe must be isolated from the diseased host and

grown in pure cultureiii. The microbe must cause the same disease when

introduced into a healthy, susceptible host

iv. The same strain of microbe must be obtained from the

newly diseased host

2. Seminal events that launched the modern age (Page 12)

a. Be able to place these events in chronological order

3. Relevance of microbiology (Page 14)

4. Groups of microorganisms (Page 15)

a. Classify Prokaryotic and Eukaryotic

b. Viruses/ prions are also microbes?YES (for the purposes of this

class)

c. Can microbes can be seen with naked eye?

5. Relationship of microbes to other forms of life (Page 16)

a. Similarities

i. Metabolism

1. glycolysis

ii. Physical structure

1. What is the accepted model for both eukaryotic and

prokaryotic membranes and who created this

model?

2. Describe the fluid mosaic model.

b. Differences

i. Size

1. more complex tends to be larger (euk > prok)

ii. Cellular complexity

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1. cellular organization, organisms such as plants,

animals, insects, fungi, etc. are much more complex

than your typical bacterium (euk > prok)iii. Metabolic diversity

1. prok > euk

6. Function of membrane enclosed systems (Page 17)

7. Presence or Absence of sterols * not in reader

a. Eukaryotes

i. present

b. Most prokaryotes

i. absentc. Exceptions

i. Mycoplasma

ii. Methanotrophs

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Week 2 material

1. Define infection (Not in reader)

a. The growth of a ________ in or on a host2. Ribosomal subunits (Page 18)

3. Significance of subunit type (Page 19)

a. What can cycloheximide be used for?

4. Peroxisome function (Not in text nor reader)

a. What can the peroxisome do?

b. What is the enzyme located in the peroxisome that is involved in

the function of the peroxisome?

5. Surface area to volume ratio (Not in reader)a. Small cells: ________ SA/V ratio

i. (Greater/ Less) efficient trafficking

ii. Results in (faster/ slower) growth rate

b. Large cells: ________ SA/V ratio

i. (Greater/ Less) efficient trafficking

ii. Results in (faster/ slower) growth rate

6. Metabolic Diversity (Page 22)

a. Similarities

i.

ii.

b. Differences

i.

ii.

iii.

7. Cytoskeletal elements (Not in reader)

a. Microfilaments:

b. Intermediate filaments:

c. Microtubules:

d. Do both eukaryotes and prokaryotes have cytoskeletal elements?

8. Nuclear apparatus and sex (Page 23)

9. Arrangement of cocci (Page 30)

a. Staphylococcus vs Sarcinae

i. Staphylococcus:

ii. Sarcinae:

10.Morphological properties of bacteria (Page 27)

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