bio lab presentation

12
Group 1

Upload: rian-bugay

Post on 26-Aug-2014

129 views

Category:

Documents


10 download

TRANSCRIPT

Page 1: Bio Lab Presentation

Group 1

Page 2: Bio Lab Presentation

• Diffusion is the process by which molecules spread from areas of high concentration, to areas of low concentration. When the molecules are even throughout a space - it is called EQUILIBRIUM

Page 3: Bio Lab Presentation

• We signed out 3 test tubes filled with hardened 5% gelatin solution from the laboratory stock room.

Fig. 1 Three test tubes with equal amounts of gelatin

Page 4: Bio Lab Presentation

• Then simultaneously, we added 1 mL of each of the following reagents into separate tubes of gelatin:

Fig. 2 Potassium Permanganate Fig. 3 Methalyne Blue Fig. 4 Congo Red

Page 5: Bio Lab Presentation

Congo Red

Methylene Blue

KMnO4

Fig. 4 Diffusioin of KMnO4, Methylene Blue, and Congo Red in gelatin

Page 6: Bio Lab Presentation

• We observed and recorded the time required for the stains to reach the bottom of each tube.

At 5 minute intervals for 1 ½ hr, we

recorded the distance travelled by each stain through the gelatiin

Page 7: Bio Lab Presentation

Table 1 Distance Travelled by Potassium Permanganate, Methylene Blue, and Congo Red

Time (min) Distance Travelled (cm)KMnO4 Methylene Blue Congo Red

5 18 mm 16 mm 23 mm

10 18 mm 16 mm 25 mm

15 20 mm 16 mm 26 mm

20 20 mm 16 mm 26 mm

25 20 mm 16 mm 26 mm

30 20 mm 16 mm 26 mm

35 20 mm 17 mm 26 mm

40 21 mm 17 mm 26 mm

45 21 mm 17 mm 26 mm

Page 8: Bio Lab Presentation

Time (min) KMnO4 Methylene Blue Congo Red

50 21 mm 17 mm 26 mm

55 17 mm 17 mm 26 mm

60 17 mm 17mm 26 mm

65 17 mm 17 mm 26 mm

70 18 mm 15 mm 26 mm

75 19 mm 16 mm 26 mm

80 19 mm 16 mm 26 mm

85 19 mm 16 mm 26 mm

90 19 mm 16 mm 26 mm

Table 1.1 Distance Travelled by Potassium Permanganate, Methylene Blue, and Congo Red

Page 9: Bio Lab Presentation

• Which reagent diffused the farthest? As we have observed in the experiment,

Congo Red is the reagent that diffused the farthest.

MW KMnO4: 158.03 g/mol MW Methylene Blue: 319.85 g/mol MW Congo Red: 696.66 g/mol

Small molecules will generally have less obstacles, thus moving faster than the larger molecules.

Page 10: Bio Lab Presentation

• What primary factor determined the rates of diffusion of the 3 reagents?

The primary factor that determined the rates of diffusion is the size of the molecules of the substances. As mentioned earlier,  the smaller the molecule, the faster it diffuses. The type of mixture is also a factor. Colloid is much compact than other solutions, that is why it is harder to diffuse substances in it.

Page 11: Bio Lab Presentation

How does diffusion in gelatin compare with diffusion in water?

Gelatin is denser than water and it is a colloid which implicates that the diffusion in gelatin would be slower in gelatin than in water.

Page 12: Bio Lab Presentation

THANK YOU!

• Bugay, Marian Louise• Carino JC Hope• Paredes, Jessica Elizabeth Marie• Quero, Allysa Kaye• Reyes, Clarissa• Villafuerte, Hazel Aracelli