bio rad water

Bio-Rad Laboratories • Water Testing

Water SafetyGuide for Bio-Rad Products in Water Testing

2011

Water SafetyGuide for Bio-Rad Products in Water Testing

ContentsPart 1 – Quality Indicators Testing

Total flora 3

E. coli – EN ISO miniaturized MPN standard 4

E. coli – Automated alternative method: XplOrer64™ System 5

E. coli/Coliforms – NF EN ISO standards 6

E. coli/Coliforms – RAPID’E.coli 2 alternative method 7

Enterococci – EN ISO miniaturized MPN standard 8

Enterococci – Automated alternative method: XplOrer64™ System 9

Enterococci – EN ISO standard 10

Enterococci – RAPID’Enterococcus alternative method 11

Part 2 – Pathogens Testing

Sulfite-Reducing Anaerobes (Clostridium) – NF EN ISO standards 12-13

Legionella spp. & Legionella pneumophila – NF EN ISO standards methods 14-15

Legionella spp. and Legionella pneumophila – Extraction for real-time PCR method 16

Legionella spp. and Legionella pneumophila – Alternative real-time PCR method 17

Legionella detection in aerosols with CIP10-M Apparatus – Bio-Rad solution 18

Legionella detection in biofilms, Amoeba – Bio-Rad technical notes 19

Pseudomonas aeruginosa – EN ISO standards 20

Pseudomonas aeruginosa – RAPID’P.aeruginosa alternative method 21

Salmonella spp. – ISO standard 22-23

Staphylococcus aureus – NF standard 24-25

This booklet is only an indication provided by Bio-Rad for the use of Bio-Rad products.It is not a contractual document.

New !

Guide for Bio-Rad Products in Water Testing | | 3

Quality Indicators TestingQuality Indicators Testing

Ordering Information

Catalog # Description

PCA (Plate Count Agar) without Dextrose/Agar 355-4437 200 ml x 6 bottles 356-4474 500 g

Total flora

Pour-Plate method

Water sample (preparation)

PCA without Dextrose/Agar

Reading/enumeration

Reading/enumeration

EN ISO 6222 (07/1999)Water quality

Enumeration of culturable micro-organisms. Colony-count by inoculation

in a nutrient agar culture medium.

68 hrs22°C

44 hrs36°C

4 Guide for Bio-Rad Products in Water Testing | |


Catalog # Description DSM + Bromophenol Blue 355-4282 18 ml x 60 tubes

DSM (Dehydrated Special Microplates Diluent) 355-3784 100 g

EDS (Distilled Water for Microplates) 355-4283 18 ml x 60 tubes

Fluoplate™ 2 Software✹ Data management software for the statistical estimation of MPN and its confidence intervalPlease contact your local representative

MUG/EC E. coli Microplates Kit 355-3785 25 opaque white plates with stickers355-3782 25 transparent plates with stickers

Long-Wave UV Lamp, Compact Portable 166-0500 1 lamp (366 nm, requires 4 AA batteries)

Portable UV Observation Chamber (without Wood Lamp) 355-0717 1 chamber

Square Petri dishes 355-0304 120 mm x 252 units

UV Observation Chamber (Wood Lamp) 355-0718 1 UV tube (366 nm)

E. coli - EN ISO miniaturized MPN standard

Water sample (dilutions in DSM or EDS)

Reading(λ = 366 nm - UV light)

UV lamp

Calculation and interpretationFluoplate™ 2 Software✹

MUG/EC (microplates)

EN ISO 9308-3 (03/1999)Water quality

Detection and enumeration of Escherichia coli and coliforms bacteria

Part 3: Miniaturized method (Most Probable Number) for the detection and enumeration

of E. coli in surface and residual water

36-72 hrs44°C

• Statistical control (Xhi2 test) of the input values• Creation of a database easily exportable into Excel™* or Access™*• Multi-language software and reports: english, french, spanish, italian and german*Excel™ is a trademark of Microsoft*Access™ is a trademark of Access Systems

✹ Key Benefits


Quality Indicators Testing



XplOrer64™ System Full Configuration (220/110V)✹ XplOrer64 System for up to 64 samples, delivered with computer, UPS, color printer, CheckN’Safe™ temperature control cells, cleaning box and 4 racks

XplOrer64™ System Configuration w/o PC (220V) XplOrer64 System 220V for up to 64 samples, CheckN’Safe™ temperature control cells, cleaning box and 4 racks

XplOrer64™ System Configuration w/o PC (110V) XplOrer64 System 110V for up to 64 samples, CheckN’Safe™ temperature control cells, cleaning box and 4 racksPlease contact your local representative

CheckN’Safe™ E.coli Kit 355-4720 9 ml x 60 tests

CheckN’Safe™ Screw Caps 359-3457 Sterile caps (1 bag of 30 units)

E. coli - Automated alternative method: XplOrer64™ System

Water sample filtration


Membrane inserting into

CheckN’Safe™ E.coli


CheckN’Safe™

E.coli

Automated real-time results analysis and interpretation with

XplOrer64™ Software

Automated detection or quantification with XplOrer64™ System✹

Automated real-time and continuous quantification of Escherichia coli in bathing

(coastal sea and inland water) and waste waters

NF VAliDAtioN according to “Protocol for the Validation of a commercial method versus a reference method in the water microbiology field” (revision 0), Attestation No BRD 07/18 - 11/09

Total E. colianalysis cycle:- bathing water: 8 hrs- waste water: 12 hrs- drinking water: 12 hrs

• First alternative method certified NF VAliDAtioN for bathing and waste water monitoring• Easy-to-use and automated method to quantify E. coli in real-time, without confirmation• Random access for up to 64 sample analyzed simultaneously • Fast Results: 2,000 E. coli/100 ml detected in 4 hrs 20 min 500 E. coli/100 ml detected within 5 hrs• Automatic expression of results according to 76/160/EEC or 2006/7/EC European Directive• Accurate alternative method compared to miniaturized MUG/ EC Microplates

✹ Key Benefits

New !

Samples poor in suspended matters (SM)

100 ml protocol

Bathing water (sea, inland) Treated waste water

Drinking waters

Samples with high SM level

10 ml protocol

Treated or untreated waste water

Non filterable water samples

1 ml protocol

Untreatedwaste water

Pipetting of 1 ml of water

sample

Direct inoculationCheckN’Safe™

E.coli

New !




BCP (Bromocresol Purple) Lactose/Broth 355-3414 10 ml x 25 tubes

BGBLB (Brilliant Green Bile Lactose Broth) 357-8024 10 ml x 25 tubes

Kovacs Reagent 355-5313 15 ml x 2 bottles

Oxidase Disks 355-3832 30 disks

Peptone Water (without Indole)/Broth 355-4180 9 ml x 25 tubes356-4334 500 g

Schubert/Broth 355-5694 8 ml x 25 tubes356-9634 500 g

Tergitol 7 (0.2%)/Supplement 356-2632 50 ml x 1 bottle356-2635 5 ml x 1 vial

TTC (0.05%)/Supplement 356-2652 50 ml x 1 bottle 356-2655 5 ml x 1 vial

TTC Tergitol 7/Agar - Base Medium 355-4687 200 ml x 6 bottles356-4454 500 g

TTC Tergitol 7/Agar - Complete Medium 356-3706 55 mm x 10 dishes356-3980 55 mm x 100 dishes

Trypto-Casein-Soy (TSA)/Agar 356-3884 90 mm x 20 dishes355-5947 200 ml x 6 bottles355-5944 8 ml x 25 slanted tubes356-4554 500 g

Tryptophane/Broth 355-4194 3 ml x 25 tubes

Tryptose Lauryl Sulfate/Broth 356-4774 500 g

E. coli/Coliforms - NF EN ISO standards


Membrane

Standard test TTC Tergitol 7

(10) yellow colonieswith yellow area

TSA10 tubes

Oxidase testOxidase

disks

Indole testKovacs reagent

Red colonies

Tryptophanebroth

10 tubes

Membrane onindole paper

+ UV

Transfer themembrane on TBA

Rapid testTSA

Water sample (dilutions)

Trouble and gas+

Troublegas +Total

coliforms

Troublegas +

Thermotolerantcoliforms

Indole test(Kovacs reagent

in Schubert)E. coli

BCP Lactose Broth or

Tryptose Lauryl Sulfate Broth

BGBLB BGBLB orSchubert


Detection and enumeration of Escherichia coli and coliforms bacteria

Part 1: Membrane-filtration method

NF T90-413 (10/1985)Water testing

Detection and enumeration of coliforms and thermotolerant

coliformsGeneral method by culture in

liquid media (MPN)

4-5 hrs36°C

19-20 hrs44°C

10-30 min

21 hrs44°C

21 hrs36°C

21 hrs(+21 hrs)36°C

24-48 hrs30°C

24-48 hrs44°C

48 hrs37°C





RAPID’E.coli 2 Kit for Water Testing 355-5296 6 bottles + 6 vials of sup.

RAPID’E.coli 2/Agar for Water Testing 356-3982 55 mm x 20 dishes

E. coli/Coliforms - Alternative method: RAPID’E.coli 2 for Water Testing


Membrane

Enumeration of typical colonies• Green colonies = Coliforms

• Grey blue to violet colonies = E. coli

BGBLB orSchubert

RAPID’E.coli 2 for Water TestingChromogenic agar medium for the direct detection and

enumeration of E. coli and coliforms, by membrane-filtrationNF VAliDAtioN according to “Protocol for the validation of a commercial method versus a reference method in the water microbiology field” (revision 0),Attestation No BRD 07/20 - 03/11

24 hrs36°C for coliforms and E. coli

24-48 hrs30°C

24-48 hrs44°C

+

RAPID' E.coli 2 Agar for Water Testing

• Certified by NF VAliDAtioN• one single plate for simultaneous E. coli and coliforms testing• Complete results within 18 ± 4 hrs at just one temperature of 36°C• Good colony coloration contrast for a rapid reading• No confirmation step required

Key Benefits

New !

New !




DSM + Bromophenol Blue 355-4282 18 ml x 60 tubes

DSM (Dehydrated Special Microplates Diluent) 355-3784 100 g

EDS (Distilled Water for Microplates) 355-4283 18 ml x 60 tubes

Fluoplate™ 2 Software✹ Data management software for the statistical estimation of MPN and its confidence intervalPlease contact your local representative

MUD/SF Enterococci Microplates Kit 355-3786 25 opaque white plates with stickers355-3783 25 transparent plates with stickers


Portable UV Observation Chamber (without Wood Lamp) 355-0717 1 chamber

Square Petri dishes 355-0304 120 mm x 252 units


Enterococci - EN ISO miniaturized MPN standard


Detection and enumeration of Enterococci in surface and waste water

Miniaturized method (Most Probable Number) by inoculation in liquid medium

Water sample (dilutions in DSM or EDS)


UV lamp

Calculation and interpretationFluoplate™ 2 Software✹

MUD/SF (microplates)

36-72 hrs44°C

• Statistical control (Xhi2 test) of the input values• Creation of a database easily exportable into Excel™* or Access™*• Multi-language software and reports: english, french, spanish, italian and german*Excel™ is a trademark of Microsoft*Access™ is a trademark of Access Systems

✹ Key Benefits





XplOrer64™ System Full Configuration (220/110V)✹ XplOrer64 System for up to 64 samples, delivered with computer, UPS, color printer, CheckN’Safe™ temperature control cells, cleaning box and 4 racks

XplOrer64™ System Configuration w/o PC (220V) XplOrer64 System 220V for up to 64 samples, CheckN’Safe™ temperature control cells, cleaning box and 4 racks

XplOrer64™ System Configuration w/o PC (110V) XplOrer64 System 110V for up to 64 samples, CheckN’Safe™ temperature control cells, cleaning box and 4 racksPlease contact your local representative

CheckN’Safe™ Enterococci Kit 355-4721 10 ml x 60 tests

CheckN’Safe™ Screw Caps 359-3458 Sterile caps (1 bag of 30 units)

Enterococci - Automated alternative method: XplOrer64™ System




CheckN’Safe™ Enterococci


CheckN’Safe™ Enterococci

Automated real-time results analysis and interpretation with

XplOrer64™ Software

Automated detection or quantification with XplOrer64™ System✹

Automated real-time and continuous quantification of intestinal Enterococci in bathing water (coastal sea

and inland water) and waste waters

NF VAliDAtioN according to “Protocol for the Validation of a commercial method versus a reference method in the water microbiology field” (revision 0), Attestation No BRD 08/19 - 11/09

Total Enterococcianalysis cycle: - bathing water: 12.5 hrs - waste water: 16 hrs

• First alternative method certified NF VAliDAtioN for bathing and waste water monitoring• Easy-to-use and automated method to quantify in real-time, without confirmation• Random access for up to 64 samples analyzed simultaneously • Fast Results: 400 Enterococci/100 ml detected in 8 hrs 30 min 250 Enterococci/100 ml detected within 9 hrs• Automatic expression of results according to 76/160/EEC or 2006/7/EC European Directive• Accurate alternative method compared to miniaturized MUD/SF microplates

✹ Key Benefits

New !

Samples poor in suspended matters (SM)

100 ml protocol

Bathing water (sea, inland) Treated waste water

Samples with high SM level

10 ml protocol

Treated or untreated waste water

Non filterable water samples

1 ml protocol

Untreatedwaste water

New !

Pipetting of 1 ml of water

sample

Direct inoculationCheckN’Safe™

Enterococci




BEA/Agar 356-3994 90 mm x 20 dishes 355-5829 100 ml x 6 bottles 356-4184 500 g

TTC (0.2%)/Supplement 356-2665 5 ml x 1 vial

Slanetz and Bartley/Agar Base Medium 355-4819 100 ml x 6 bottles 356-4934 500 g

Slanetz and Bartley/Agar Complete Medium 356-3716 55 mm x 10 dishes 356-3981 55 mm x 100 dishes


ReadingTransfer the membrane on BEA

Reading/enumeration

Membrane

Slanetz & Bartley

NF EN ISO 7899-2 (08/2000)Water quality

Detection and enumerationof intestinal Enterococci

Part 2: Membrane-filtration method

44 hrs36°C

2 hrs44°C

Enterococci - EN ISO standard





RAPID’Enterococcus/Agar 355-4409 100 ml x 6 bottles


Enumeration of typical colonies• Blue colonies = Enterococcus

Membrane

RAPID’Enterococcus

RAPID’Enterococcus

Chromogenic agar for the direct detection and enumeration

of intestinal Enterococci, by membrane-filtration

44 hrs ± 4 hrs44°C

8-10 ml per plate (Ø = 55 mm)

Enterococci - RAPID’Enterococcus alternative method

• Direct identification and enumeration of Enterococci• No confirmation step required• Ready-to-use medium• Save time and materiel

Key Benefits


Sulfite-Reducing Anaerobes (Clostridium) - NF EN ISO standardsOrdering Information


Columbia/Agar 356-4674 500 g

Meat-Liver Sulfite Iron/Agar Complete Medium 355-4770 100 ml x 6 bottles 355-4777 20 ml x 25 tubes355-4794 7.5 ml x 25 tubes 356-9654 500 g

TSC without D-Cycloserine/Agar Base Medium 355-4419 100 ml x 6 bottles356-9644 500 g

Water sample (preparation)

Reading

Reading/enumeration

Meat-Liver Sulfite Iron

NF T90-415 (10/1985)Water testing

Detection and enumeration of spores of sulfito-reducing anaerobic bacteria

and of ClostridiumGeneral method by incorporation of agar

in deep tubes

NF EN 26461-1 (07/1993)Water quality

Detection and enumeration ofspores of sulfito-reducing anaerobic

micro-organisms (Clostridia)Part 2: Membrane-filtration method

24 hrs37°C

48 hrs37°C

10 min80°C

Anaerobicconditions

Anaerobicconditions

Meat-Liver Sulfite Iron Agar

Pathogens Testing


Sulfite-Reducing Anaerobes (Clostridium) - NF EN ISO standards

Water sample (preparation) Water sample (preparation)

Reading/enumeration

Reading/enumeration

Confirmation (Columbia Agar, Nutrient Agar 2.1%)

TSC without D-Cycloserine

DRCM


Membrane

NF EN 26461-1 (07/1993)Water quality

Detection and enumeration ofspores of sulfito-reducing anaerobic

micro-organisms (Clostridia)Part 2: Membrane-filtration method

ISO/DIS 6461-2 (06/2005)(ISO 6461-2:1986 review)

Water qualityDetection and enumeration of

Clostridium perfringensPart 2: Membrane-filtration method

44 hrs37°C

15 min75°C

15 min60°C

15 min60°C

Anaerobicconditions

Anaerobicconditions


Legionella spp. & Legionella pneumophila – NF EN ISO standards methods

Water sample

Direct sample analysis

Ultrasonication or raising or centrifugation of the membrane

Confirmation of Legionella pneumophilaMonoFluo™ Kit or

Legionella pneumophila Latex kit

BCYE Biplate

0.2 mlGVPC

0.2 ml 1/10for dirty water

GVPC

ISO 11731 (05/1998): Water quality

Detection and enumeration of Legionella



BCYE Biplate/Agar 356-3718 90 mm x 20 dishes

BCYE with L-Cysteine/Agar 356-3720 90 mm x 20 dishes

BCYE without L-Cysteine/Agar 356-3722 90 mm x 20 dishes

Columbia Agar + Horse Blood/Agar 356-3804 90 mm x 20 dishes

GVPC/Agar 356-3717 90 mm x 20 dishes356-3719 90 mm x 100 dishes

Legionella pneumophila Latex Kit 356-2790 50 tests

Legionella pneumophila and Legionella Species Latex Kit 356-2795 50 tests

MonoFluo™ Kit Legionella pneumophila 353-2514 24 tests

Nutrient/Agar (For Water Testing) 356-3786 90 mm x 20 dishes


Portable UV Observation Chamber (Without Wood Lamp) 355-0717 1 chamber


Pathogens Testing


Legionella spp. & Legionella pneumophila – NF EN ISO standards methods

Water sample

Membrane filtration (1 L)

Ultrasonication or raising or centrifugation of the membrane

Confirmation of Legionella spp.

Confirmation of Legionella pneumophilaMonoFluo™ Kit or

Legionella pneumophila Latex kit

Concentrate

direct inoculation0.1 mlGVPC

BCYE with L-Cysteine

BCYE without L-Cysteine

(or Blood Agar or Nutrient Agar)

heat treatment 0.1 ml GVPC

acid treatments0.2 mlGVPC

NF T90-461/A1 (04/2006): Water quality

Detection and enumeration of Legionella spp. and Legionella pneumophila

Method by direct inoculation and after concentration by membrane filtration

or centrifugation

NF EN ISO 11731-2 (07/2008): Water quality

Detection and enumeration of Legionella

Part 2: Direct membrane-filtration method for water with low bacterial counts

48 hrs36°C

Note: legionella Latex Kit Quick Guides are available on request

8-10 days36°C

or

+




Aquadien™ Kit✹ 357-8121 96 tests

Also available:Protocol for clogging samples

Aquadien W2, Wash Solution 357-8119 48 tests

Purification of DNA using Ultrafiltration

Aquadien KitLysis of bacteria using thermal shock

Elution of DNA for real-time PCR analysis

Aerosol sample Biofilm sample Water sample

Extraction stepAquadien DNA extraction Kit

NF VAlIDATION certified accordingWater quality - Detection and quantification of

by concentration and genic amplificationNo BRD 07/15 -05/11 ;

15 min95°C

2 x 10 min

Legionella DNA extraction Kit - Bio-Rad solution

95°C


• High extraction yield• Compatible with all type of water (drinking water as well as cooling tower)• NF VAliDAtioN certified• Ready-to-use reagents• Simple protocol

✹ Key Benefits

Pathogens Testing




Reagent kitsiQ-Check™ Quanti Legionella spp. Kit 357-8102 96 tests

iQ-Check™ Quanti L. pneumophila Kit 357-8103 96 tests

iQ-Check™ Screen Legionella spp. Kit 357-8104 96 tests

iQ-Check™ Screen L. pneumophila Kit

357-8105 96 tests

Instruments and softwareReal-time PCR Cycler 359-3990 Industrial Diagnostic CFX 96™ Real-Time PCR Detection System, 96 wells

Software 359-3893 CFX Manager™ Software Industrial Diagnostic Edition

DocumentationMethod Validation Guide(NF T90-417 / 2010) Please contact your local representative

Real-time PCR amplification

Analysis stepiQ-Check™ Legionella real-time PCR Kits

to NF T90-417 (04/2010):Legionella spp. and/or Legionella pneumophila by polymerase chain reaction (PCR)BRD 07/16 -05/11

Legionella spp. and Legionella pneumophilaAlternative real-time PCR method

DNA Extraction Aquadien Kit

Screening

iQ-Check™ Screen Legionella spp.

iQ-Check™ Screen L. pneumophila

Quantification

iQ-Check™ Quanti Legionella spp.

iQ-Check™ Quanti L. pneumophila

Automatic result analysis and interpretation

• Just 5 hours to the result with no confirmation• Detection of Legionella spp. and L. pneumophila in the same assay• A 96-wells format adaptable to all routine throughput• Ready-to-use reagents • Fully automated results analysis• Reference Material provided• Connected to the Standard Reference Material• NF VAliDAtioN certified

Key Benefits

New !




ReagentAquadien™ Lysis Solution Please contact your local representative

InstrumentsCIP10-M* Air Sampler/220V✹ 359-3084 1 sampler and accessories for 3 sampling

CIP10-M* Air Sampler/110V 359-3094 1 sampler and accessories for 3 sampling

Legionella DNA ExtractionAquadien™ Kit

(See p. 16)

Real-time PCR amplificationiQ-Check™ Legionella

KitsDetection or quantification

(See p. 17)

Legionella 2009 posterThe hospital tap waters: a risk of Legionella contami-nated aerosol inhalation

M. Reyrolle, C. landelle,C. Mazure, MC. Nicolle, S. Jarraud, J. Etienne, P. Vanhems

Aerosol samplingCIP10-M

Real-time NF T90-471 Standard Culture ISO 11731

CIP10-M

Method for the monitoring of airborne micro-organisms, especially Legionella in aerosols from cooling towers and droplets

Legionella detection in aerosols with CIP10-M Air Sampler - Bio-Rad solution

CIP10-M Air Sampler

* CIP10-M is a trademark of ARELCO

• Simulates human breath• Ergonomic• Portable• Free of cross contamination• Suitable for both culture media (on-going studies) and real-time PCR analysis

✹ Key Benefits

Pathogens Testing


Legionella detection in biofilms, amoeba – Bio-Rad Technical Notes

Technical note # Description

16249 Bio-Rad’s Legionella Testing Solution French version available only

16186 Aerosols Testing

16995 Biofilms Testing

16225 Intra-amoebic Legionella

Legionella 2009 Poster The hospital tap waters: a risk of Legionella contaminated aerosol inhalation M. Reyrolle, C. landelle, C. Mazure, MC. Nicolle, S. Jarraud, J. Etienne, P. Vanhems

Method Validation Guide (T90-471 / 2010) Please contact your local representative


Pseudomonas aeruginosa - EN ISO standards

Membrane

Reading/enumeration

Reading/enumerationammonia producing

colonies

Reading at λ = 366 nm UV lamp

Enumerationfluorescent colonies

Oxidase testOxidase disks

King B

Acetamide Broth

Nessler reagent

CN Agar

NF EN ISO 16266 (08/2008)Water quality

Detection and enumeration of Pseudomonas aeruginosa

Membrane-filtration method

5 days37°C

48 hrs37°C

24 hrs37°C

24 hrs37°C

Nutrient Agar (water testing)



UV lamp

Pathogens Testing


Membrane

Enumeration of typical colonies:Blue to blue-green colonies

RAPID’P.aeruginosa✹

RAPID’P.aeruginosaChromogenic agar medium

for the direct detection and enumeration of Pseudomonas aeruginosa by membrane-filtration

22-30 hrs36°C



Acetamide/Broth 355-4355 5 ml x 25 tubes

CN/Agar 356-3915 55 mm x 10 dishes355-6034 200 ml x 6 bottles356-4899 500 g

Equipment for Fluorescence Detection 335-0717 Portable UV observation chamber (without wood lamp)335-0718 Wood lamp


King B/Agar 355-5278 7 ml x 25 tubes

Nessler Reagent 356-4194 100 ml x 1 vial Nutrient/Agar (for Water Testing) 356-3786 90 mm x 20 dishes

Oxidase Disks 355-3832 box of 30 disks

RAPID'P.aeruginosa/Agar✹ 356-3984 55 mm x 20 dishes356-4900 500 g


• High specificity for a direct enumeration of Pseudomonas aeruginosa• Complete results within 22-30 hrs• Good colony coloration contrast for a rapid reading• No confirmation step required

✹ Key Benefits

New !

Pseudomonas aeruginosaRAPID’P.aeruginosa alternative method

New !


Salmonella spp. - ISO standard

ISO 19250 (07/2010)Water quality

Detection and enumeration of Salmonella species



Brilliant Green Red Lactose Phenol (or Kristensen or Edel and Kampelmacher)/Agar 356-4464 500 g

Buffered Peptone Water/Broth 355-4179 25 ml x 6 bottles 356-4684 500 g

Kligler-Hajna/Agar 355-5378 10 ml x 25 slanted tubes356-4844 500 g

LDC-ODC-ADH/Broth 355-3725 5 ml x 3 bottles

MKTTn (Muller-Kauffmann Tetrathionate Novobiocin)/Broth 355-6140 10 ml x 50 tubes 356-4714 500 g

Nutrient Agar (2.1%) without NaCl 356-4485 500 g Physiological Sterile Water (0.85%) 355-4164 9 ml x 25 tubes

RVS (Rappaport Vassiliadis Soya)/Broth 355-5773 10 ml x 25 tubes 356-4324 500 g

TSI (Triple Sugar Iron)/Agar 356-4384 500 g

XLD (Xylose Lysine Desoxycholate)/Agar 356-9124 500 g

No filtration(if volume < 10 ml)

Biochemical (TSI, LCD, Kliger-Hajna, urea agar)

and if appropriate serological confirmation

First medium XLD

Nutrient Agar (2.1%)

Buffered Peptone Water

RVS Broth or MKTTn24-48 hrs41.5°C

24 hrs36°C

Pathogens Testing


Salmonella spp. - ISO standard

ISO 19250 (07/2010)Water quality

Detection and enumeration of Salmonella species

Water sample

No filtration(if volume < 10 ml)

Filtration(if volume > 10 ml)

Membrane

Biochemical (TSI, LCD, Kliger-Hajna, urea agar)

and if appropriate serological confirmation

Second medium left to choice Kristensen

(Brilliant Green/Phenol Red Agar)

First medium XLD

Nutrient Agar (2.1%)

Buffered Peptone Water

RVS Broth or MKTTn

18 hrs36°C

24 hrs36°C

24 hrs36°C

24 hrs36°C


Staphylococcus aureus – NF standard

Membrane

Enumeration of white colonies with yellow

halo/Gram+

Transferthe membrane

ConfirmationLatex Pastorex™ Staph+

ConfirmationBrain Heart Infusion

Coagulation reading:Rabbit Plasma

Enumeration of black colonies with clear

halo/Gram+

or

Chapman Mannitol (Water) Baird-Parker

XP T90-412 (06/2006):Water quality

Detection and enumeration of pathogenic StaphylococciMembrane-filtration method

48 hrs37°C

48 hrs37°C



Baird-Parker/Agar Base Medium 356-4814 500 g

Baird-Parker/Agar Complete Medium 356-3991 90 mm x 20 plates

Baird-Parker for RPF/Agar Complete Medium 356-3996 90 mm x 20 plates357-8618 90 ml x 6 bottles + 6 vials supplement

BHI (Brain-Heart Infusion)/Broth 355-3664 10 ml x 25 tubes 356-4014 500 g

Chapman Mannitol (for Water Testing)/Agar 356-3926 55 mm x 10 dishes 356-4824 500 g

Additives

Egg yolk with potassium tellurite355-4201 5 ml x 1 vial355-4205 25 ml x 1 bottle

Pastorex™ Staph Plus Kit 355-6353 5 x 50 tests 355-6356 50 tests

Rabbit Plasma Kit 355-6352 Pack for 20 reactions

RPF (Rabbit Plasma Fibrinogen)/Supplement 356-4618 10 vials

Sulfamethazine Solution (0.2%)/ Supplement 356-2682 2.5 ml x 1 vial



Pathogens Testing

Staphylococcus aureus – NF standard

Membrane

ConfirmationBaird-Parker + RPF

Enumeration of black or grey colonies surrounded by a whitish opaque halo/

Gram+

Enumeration of black colonies with clear

halo/Gram+

Direct enumeration of black or grey colonies surrounded

by a whitish opaque halo/Gram+

or or

Baird-Parker Baird-Parker + RPF

XP T90-412 (06/2006):Water quality

Detection and enumeration of pathogenic StaphylococciMembrane-filtration method

48 hrs37°C

48 hrs37°C

48 hrs37°C

48 hrs37°C



Treated Water Non-Treated Water

Main types of water

Network TapIndustrial Process

Artificial Surface Bottled Superficial (Surface)

Bathing Waste Cooling Towers Aerosols

Origins of water Drinking watertreated cooling towers, process,

sanitary water

Spa, hydrothe-rapy, swimming pool, whirlpools

Natural mineral and source water

lakes, riversCoastal sea,

estuarine, inland water

Residual raw waters, sludge, soil, treatment plant effluents

Non treated cooling towers

Drops from cooling towers,

tap water

Turbidity + + + + +++ +++ +++ ++++ ++

Microbial load + + + + ++ ++ +++ +++ +++

Typical methods MF (EUR) P/A (US) MF MF MF MF/MPNMF/MPN

impedance MPN/PCR

impedanceMPN/PCR PCR

Typical quality indicators

total floraE. coli

ColiformsEnterococci

total floraE. coli


total floraE. coli


total floraColiforms(E. coli)

total floraE. coli


total floraE. coli


total floraE. coli


total flora total flora

Typical pathogens

LegionellaP.aeruginosaSalmonella

SRA

LegionellaP.aeruginosa Salmonella

SRA

LegionellaP.aeruginosa Staph. aureus

Salmonella SRA

LegionellaP.aeruginosa

Salmonella SRA

-LegionellaSalmonella

Legionella Legionella

Legends:

SRA = Sulfito-Reducing Anaerobes MF = Membrane filtration MPN = Most Probable Number P/A = Presence/AbsencePCR = Polymerase Chain Reaction

Micro-organisms generally detected and methods the most commonly used according to the origin of water sample


Treated Water Non-Treated Water

Main types of water

Network TapIndustrial Process

Artificial Surface Bottled Superficial (Surface)

Bathing Waste Cooling Towers Aerosols

Origins of water Drinking watertreated cooling towers, process,

sanitary water

Spa, hydrothe-rapy, swimming pool, whirlpools

Natural mineral and source water

lakes, riversCoastal sea,

estuarine, inland water

Residual raw waters, sludge, soil, treatment plant effluents

Non treated cooling towers

Drops from cooling towers,

tap water

Turbidity + + + + +++ +++ +++ ++++ ++

Microbial load + + + + ++ ++ +++ +++ +++

Typical methods MF (EUR) P/A (US) MF MF MF MF/MPNMF/MPN

impedance MPN/PCR

impedanceMPN/PCR PCR

Typical quality indicators

total floraE. coli


total floraE. coli


total floraE. coli


total floraColiforms(E. coli)

total floraE. coli


total floraE. coli


total floraE. coli


total flora total flora

Typical pathogens

LegionellaP.aeruginosaSalmonella

SRA

LegionellaP.aeruginosa Salmonella

SRA

LegionellaP.aeruginosa Staph. aureus

Salmonella SRA

LegionellaP.aeruginosa

Salmonella SRA

-LegionellaSalmonella

Legionella Legionella

Micro-organisms generally detected and methods the most commonly used according to the origin of water sample

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© Bio-Rad 25 17932 Rev F 07/2011Printed in France with soy-based inks on paper from sustainably managed forests

bio rad water

Documents

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enterococci en iso standard

miniaturized method

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legionella detection

use of biorad products

water testingcontentspart