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Caliper Life Sciences Bioware Ultra Cell Line Models Offer the Ability to:

• Monitor early tumor development

• Monitor tumor growth and metastases in vivo

• Quantify tumor burden in the whole animal

• Follow responses to therapeutic treatments non-invasively in longitudinal studies using the same cohorts of mice.

Murine Pathogen FreeAll Caliper Life Sciences cell lines are confirmed to be pathogen free by the IMPACT Profile I (PCR) at the University

of Missouri Research Animal Diagnostic and Investigative Laboratory.

Human Prostate: LNCaP-luc2

LnCaP-luc2 is a luciferase expressing cell line which was stably transfected with firefly luciferase gene (luc2).

The cell line was established by transducing lentivirus containing luciferase 2 gene under the control of human

ubiquitin C promoter. This cell line can be used in vivo to establish:

• Subcutaneous tumor models

• Subcutaneous tumor models with metastasis (SCID-bg mice)

• Orthotopic prostate tumor model

Gnral Information

Bioware Ultra Cell Line LNCaP-luc2

DeSIGNATION LNCaP-luc2

Tisse Hmn: prostte; metsttic site: left sprclviclr lymph node; crcinom

Gene Trnsfer Vehicle pGL4 lc2 Lentivirs

Biolminescence In Vitro approximtely 30 photons/sec/cell.Exct nmber will vry depending on imging nd cltring conditions.

Recommended MediRPMI 1640 (aTCC Ctlog No. 30-2001) Spplemented withUNHEATED FBS t finl concentrtion 10%

Cell Dobling Time 19 hors

Other RecommendtionsWhen initilly thwing, se T25 flsk. Cells shold be redy to expnd next dy.We recommend not sing ny ntibiotics in the medi.

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CELL LINE LNCaP-LuC2

In Vitro Stability of Lucifras exprssionWeek 3 Luciferase expression

Average photons/sec/cell: 30

Figur 1. 5x104 cells were plated per well in black-walled 24-wellplates. Cells were incubated for 37 °C for recovery overnightand luciferase assay was performed using an IVIS Spectrum.Each experiment was done in quadruplicates. Bioluminescencedata was analyzed using the Living Image 3.0 software.

Growth Curv of LnCaP-luc2

Figur 2. 1x105 cells were plated on T25 flasks andthe total numbers of cells were counted every24 h using a Nexcelom automatic cell counter.Cell numbers were plotted in logarithmic scale.Proliferation rate was calculated using a previouslyreported formulation.

Contact Information:

If yo hve ny qestions regrding these cell lines, plese contct Cliper t 508-497-6592 or e-mil: [email protected]

www.caliprLS.com

References

1. Cthbert nd Lipsky (1997) Regltion of prolifertion nd Rs locliztion in trnsformed cells by prodcts of mevlonte metbol ism.Cncer Reserch 37:3498-3505.

2. Sherley, Stdler, nd Stdler (1995) a qntittive method for the nlysis of mmmlin cell prolifertion in cltre in terms of dividingnd non-dividing cells. Cell Prolifertion 28:137-144.

3. Co et l. (2006) In vivo visliztion of embryonic stem cell srvivl prolifertion, nd migrtion fter crdic delivery.Circltion 113:1005-1014.

4. pGL4 lciferse reporter vectors technicl mnl. TM 250. Promeg Corp. (2007)

0

10

20

30

40

50

Week 1 Week 2 Week 3 Week 4

P h o t o n s / S e c

/ C e l l

0.E+00

5.E+04

1.E+05

2.E+05

2.E+05

3.E+05

3.E+05

4.E+05

0 24 48 72 96

Hours

T o t a l N u m b e r o f C e l l s

Parental

LnCaP-luc2

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CELL LINE LNCaP-LuC2

Disclaimr

This prodct is provided nder license from Promeg Corportion nd The Regents of the university of Cliforni.

under the terms of Promeg license, the se of this prodct nd derivtives thereof is strictly limited to in vivo reserch se. Reserchers my se

this prodct in their reserch nd they my trnsfer derivtives to others for reserch se provided t the time of trnsfer copy of this lbel license

is given to the recipients nd the recipients gree to be bond by the terms nd conditions of this lbel license. In ddition, reserchers performing

in vivo biolminescent imging mst do one of the following: (1) se lminescent ssy regents prchsed from Promeg or Cliper Life Sciences

for ll determintions of lminescent ctivity reslting from the reserch se of this prodct nd its derivtives; or (2) contct Promeg to obtin license for the se of the prodct nd its derivtives in conjnction with lminescent ssy regents not prchsed from Promeg or Cliper Life

Sciences. No rech-throgh pyments shll be owed to Promeg relting to n orgniztion’s commerciliztion of prodcts tht re discoveries

reslting from the reserch se of this prodct or its derivtives, provided tht sch prodcts of the orgniztion do not fll within the vlid clims of

ny issed ptents ssigned to or licensed by Promeg, or tht sch commerciliztion wold not be violtion of the terms of this lbel license. No

right to se this prodct for ny dignostic, therpetic, or commercil ppliction is hereby conveyed to the prchser of this prodct. Commercil

ppliction is defined s ny nd ll ses of this prodct nd derivtives by prty for monetry or other considertion nd my inclde bt is not

limited to se in: (1) prodct mnfctre; or (2) to provide service, informtion or dt; nd/or (3) resle of the prodct or its derivtives, whether

or not sch prodct or its derivtives re sold for se in reserch. If the prchser is not willing to ccept the conditions of this limited lbel license,

Cliper Life Sciences is willing to ccept the retrn of the nopened prodct nd provide the prchser with fll refnd. However, in the event

the prodct is opened, then the prchser grees to be bond by the conditions of this limited lbel license.

Byer cknowledges tht the wild-type nd mtnt recombinnt Coleopter lciferse ncleic cid nd protein (“uC Ptented Mteril”) is the

sbject of u.S. Ptent Nos. 5,583,024, 5,674,713 nd 5,700,673, ssigned to The Regents of The university of Cliforni.

The uC Ptented Mteril, or ny mteril tht contins or incorportes the uC Ptented Mteril, my not be trnsferred or licensed to ny other

prty, or be sed for commercil prposes by ny other prty, withot commercil license or the express written consent of The Regents of The

university of Cliforni.

The Mterils my be sed solely for internl reserch, nd no right to se the Mterils for ny hmn in vivo , dignostic, therpetic, or commercil

ppliction is hereby conveyed to Byer.

additionlly, the Byer my not:

(1) se the Mterils in the corse of providing service or dt to third prties;

(2) trnsfer the Mterils to ny third prty or to ny plce other thn Byer’s premises;

(3) ttempt to lter, modify or re-engineer the Mterils in ny wy, or extrct or trnsfer ny genetic mteril from the prodct to

nother orgnism; or

(4) sblicense the rights grnted herein.

• Buyer is not prohibited from using the Materials to discover or develop products that it intends to sell for consideration as long as those products

do not contin ny Mterils.

• If Buyer has purchased breeding rights to an animal model, Buyer may have the animal model bred by a third party, provided that such third party

(i) does not se the niml model for ny prpose other thn breeding for the benefit of Byer,

(ii) destroys or retrns the nimls pon conclsion of the breeding services, nd

(iii) is otherwise leglly bond by the terms of this lbel license.

• Buyer agrees that the Materials are and shall be owned and/or controlled by Caliper Life Sciences, not by Buyer, and that these terms and

conditions crete bilment with Byer with respect to ny nd ll sch Mterils.

• Buyer agrees that all intellectual property rights relating to the Materials (including, but not limited to, those rights concerning the composition,

methods of prodction, or ses, of the Mterils) re nd shll contine to be owned nd/or controlled by Cliper Life Sciences nd not by

Byer.

• However, Caliper Life Sciences shall not claim ownership of any invention made by Buyer using the Materials but which relates to subject matter

other thn the Mterils.

• Buyer may terminate this bailment at any time upon written notice, and Caliper Life Sciences may terminate this bailment in the event of breach

of this lbel license by Byer.

• In the event of termination hereof, Buyer shall destroy or return all Materials to Caliper Life Sciences.

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Tel: 1.508.435.9500

Email: [email protected]

©2011 Caliper Life Sciences, Inc. All rights reserved.

Caliper, the Caliper logo, IVIS, S pectrum and Bioware

are tradenames and/or trademarks of Caliper Life

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