bioanalyzer and tapestation summit - agilent · webpage) • vortexer with adaptor • plastic...

Bioanalyzer and Tapestation Summit March 31, 2016 Santa Clara, CA

Rev. 1 [March 9, 2016]

For Research Use Only. Not for use in diagnostic procedures.

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Agenda

2

Welcome

o 10:00 - 12:00 Overview of Electrophoresis Products- Bioanalyzer & TapeStation – Comparison of Systems

Sample QC in Multiple Workflows

Bioanalyzer Tips & Tricks

Bioanalyzer Troubleshooting

Tapestation Tips & Tricks

o 12:00-2:00 Rotations Between:

Lunch

Data Analysis Workshop

Tapestation Demo

Bioanalyzer Demo

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Bioanalyzer & TapeStation Portfolio

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Microfluidics Product Portfolio

2200 TapeStation System -

ScreenTape Technology

• fully scalable

• 96-well plate compatible

• ready-to-use

4200 TapeStation System –

ScreenTape Technology • Introducing the new TapeStation system

• Unattended walk away operation with fully

automated sample processing for up to 96 samples.

2100 Bioanalyzer System –

Electrophoresis in microchannels • separation according to mobility (size)

• cell counting (pressure driven)

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AGILENT 2100 BIOANALYZER SYSTEM

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The Agilent 2100 Bioanalyzer System

First commercially available Lab-on-a-Chip product

Introduced 1999

Analysis of biomolecules: DNA, RNA, Proteins and Cells

Established standard for the analysis of RNA

Standard for sample QC in Next-Generation Sequencing workflows

> 33,000 citations

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2100 Bioanalyzer System One platform – Endless possibilities Analytical Platform with exchangable cartridge design - four application

areas by easy switch

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Bioanalyzer Kit Portfolio

Protein Assays: • Sizing

• Quantitation

• Cell lysates, column fractions,

purified proteins, antibodies etc.

• 10 samples in 25-30 min.

P230 P80

DNA Assays: • Sizing

• Quantitation

• PCR products, digests,

larger DNA fragments

• 11-12 samples in 30-45 min.

DNA1000 DNA7500 DNA12000

RNA Assays:

• Quantitation (Sizing in Small RNA)

• Total RNA, mRNA

• Purity & integrity

determination

• 11-12 samples in 30 min.

•RNA Integrity number (RIN)

6000 Nano 6000 Pico Small RNA HSP 250 High Sensitivity

DNA

Electrophoretic Separations

Cell Assays: • Analysis of 6 samples in 30 minutes

• Two color detection

• Analysis of protein expression in cells

Flow Cytometry

Flow Cytometry

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AGILENT TAPESTATION SYSTEMS

2200 TapeStation System

AND


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Agilent TapeStation Systems – Ease of Use:

Place ScreenTape and tips in

the TapeStation

ScreenTape

TapeStation

TapeStation Software

Place prepared samples in

the TapeStation and press

‘Start’ on the instrument

controller software

View analyzed results in around

1 min per sample

1

2

3

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What technology did Agilent acquire?

The TapeStation system and……….

………..ScreenTape assays

Automated gel electrophoresis system with flexible throughput and 96 well

plate compatibility. DNA, RNA and Protein analysis.

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Introducing the 4200 TapeStation System…

Complete System including

• Laptop

• 4200 TapeStation Software

(no license - free upgrades on

webpage)

• Vortexer with adaptor

• Plastic consumable start up kit

• ScreenTape Assay Quick Guides

• TapeStation Test Tape

• I&F service bundle

Made in Germany

Size: W45 x H50 x D60cm

Weight: 25kg

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100 to 6000b

2µl of sample

HS RNA

100 to 6000b

1µl of sample

RNA

LOD

100 pg/µL

LOD

5 ng/µL

D5000

LOD

0.1 ng/uL

100 to 5000bp

1µl of sample

0.1 – 50ng/µl

LOD

0.5 ng/uL

10 – 100ng/µl

Genomic

DNA 200 to >60kbp

1µl of sample

DNA

Integrit

y

Numbe

r (DIN)

ScreenTape Assay Portfolio TODAY…

P200 10 to 200kDa

2µl of sample

LOD

5 ng/uL

Protein

100–1000ng/µl IgG

DNA RNA

500–10,000pg/µl 25–500ng/µl

HS D1000

LOD

5 pg/uL

35 to 1000bp

2µl of sample

10 – 1000pg/µl

D1000

LOD

0.1 ng/uL

35 to 1000bp

1µl of sample

0.1 – 50ng/µl

HS D5000

LOD

5 pg/uL

100 to 5000bp

2µl of sample

10 – 1000pg/µl

*2200 TapeStation obsolescence by end of 2016*

4200/2200 TapeStation 2200 ONLY

RNA Integrity Number

equivalent (RINe)

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A new standard for genomic DNA QC - the DNA Integrity Number (DIN)

www.agilent.com/genomics/ArtorScience

DIN functionality for the Genomic DNA

ScreenTape assay.

• Automated numerical software assessment of

sample integrity.

• Independent of sample concentration and analyst.

• Full range of eukaryotic samples from degraded to

intact gDNA.

Benefits:

• Easy interpretation of results.

• Ensures repeatability of experiments.

• Enables comparison of samples by

defining a DIN value or threshold for

specific type of samples or preparation

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http://www.agilent.com/genomics/ArtorScience

Agilent PLuF NGS QC System offering E

ase o

f u

se

Established Standard

for NGS sample and

library QC

Automation & Throughput

2100 Bioanalyzer

system

2200 TapeStation

system

• Ready-to-use ScreenTape

Technology

• Scalable throughput 2-96

samples

• Genomic DNA analysis

• DNA Integrity Number (DIN)

4200 TapeStation

system

• 96 sample walk away system

• Ready-to-use ScreenTape

Technology

• Scalable throughput 1-96

samples

• Genomic DNA analysis

• DNA Integrity Number (DIN)

• Increased product portfolio

with D5000 / HS D5000 ScreenTape devices need to be

manually exchanged for 96 well

plate run

(hands on every 20-25 minutes)

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Hardware

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Hardware

2100 Bioanalyzer

System

4200 TapeStation

System HW Product Structure 2100 Electrophoresis Bioanalyzer

Instrument (G2939AA)

• no PC

• reduced I&F service

2100 Bioanalyzer bundles with desktop PC

(G2940CA) or laptop (G2943CA)

• systems require cartridge for

• electrophoresis (G2947CA) or for

• flow cytometry (G2948CA)

• full I&F service

HW Product Structure 4200 TapeStation System (G2991AA)

• DNA and RNA analysis.

System includes laptop, software,

accessories, initial set of consumables.

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Software

Both software offer great

functionality.

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PC and software

2100 Bioanalyzer

System

4200 TapeStation

System 2100 Expert Software • Revision B.02.08 SR3

• Instrument control and data analysis

• Security Pack / 21CFR11 compliance

(G2949CA)

TapeStation Software • Revision A.02.01

• Separate instrument control and data

analysis software packages

• 21 CFR Part 11 compliance not available

Available for download from www.agilent.com/genomics/tapestation-SW www.agilent.com/genomics/2100expert

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http://www.agilent.com/genomics/tapestation-SW



http://www.genomics.agilent.com/article.jsp?pageId=2354

http://www.agilent.com/genomics/2100expert

Software overview

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TapeStation Software - Overview


CONTROLLER


ANALYSIS

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Required Consumables Software calculates the required

consumables based on number of

samples for analysis

Automatic Assay

Selection Barcode recognises assay type

and available number of

ScreenTape lanes for analysis

Intuitive sample selection Full flexibility for sample selection

independent on sample location.

Easy population of sample

descriptions Sample names can be entered by

copy/paste or imported from a

previous file

System status &

Observations Status, warnings and errors are

displayed in the task bar

Agilent 4200 TapeStation Controller Software Easy and automated

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Navigation pane Navigate through experiments and

individual lanes. Lanes can be selected in

any order from the Plate Selector.

Analysis Functions Functions are navigated

through a context sensitive

ribbon.

Result Presentation Customisable views of gel

image, eletropherogram

and data table.

Gel and

Electropherogram

View Results can be viewed in

familiar gel view or

Electropherogram view.

Agilent TapeStation Analysis Software Intuitive and familiar

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Assays

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Current Assay Portfolio

DNA1000 DNA7500 DNA12000 High

Sensitivity DNA

6000

Nano

6000 Pico Small

RNA

P230 P80 HSP 250

Flow

Cytometry

DNA assays

RNA assays

Protein assays

Flow Cytometry

Assay

HS D1000 D1000 Genomic DNA

HS RNA RNA

P200

D5000 HS D5000

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Workflow Overview

Prepare gel-dye

mix Prime chip Load chip

Select assay

RUN

Time (minutes) 0 60

1 = 10-12 samples

in 25-40

minutes

Prepare samples

Load tips and

samples RUN

DNA Buffer

1 = 1-16 samples

in 10-20 minutes

2100 Bioanalyzer System


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= 96 samples in < 90-180 minutes 1

RNA Assay

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RNA Profile Comparison

o Close correlation between 2100 Bioanalyzer and 2200

TapeStation system

Agilent 2100 Bioanalyzer instrument

RNA 6000 Nano Assay

Agilent 2200 TapeStation System

RNA ScreenTape Assay

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Resolution alignment

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Example Gel Images from the

Bioanalyzer (BioA) and

TapeStation (TS)

Gel image and profile overlay of Eukaryotic RNA (A1 and

Blue) and Eukaryotic/Prokaryotic RNA mixture (B1 and Red)

A1 B1

o Figures below show how much closer the RNA ScreenTape assay is to the

resolution of the RNA assays on the 2100 Bioanalyzer

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RIN vs. RINe correlation

o Similar to Eukaryote,

Prokaryote samples subjected

to heat degradation and

dilution across concentration

range

o >200 individual RNA data

points (not shown)

o Median error < ±0.4 RIN units

Switching analysis mode is

easy!

o Can now be done in the

analysis software – button

shown below:

NOTE: Depending on the RNA tissue type, profiles may vary and

RINe value might be slightly different from RIN

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DNA Assay

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DNA Profile Comparison Bioanalyzer

HSDNA assay DNA fragment

Tapestation

HSD1000 assay DNA fragment

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Bioanalyzer

SSEL Sheared DNA – HSDNA assay

Tapestation

SSEL Sheared DNA - HSD1000 assay

SSEL Precapture library - HSDNA assay SSEL Precapture library - HSD1000 assay

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DNA Profile Comparison

Want to know more?

Visit the Bioanalyzer & TapeStation website for most recent

information on products, consumables and assays

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http://agilent.com/genomics/bioanalyzer

http://agilent.com/genomics/tapestation

Rev. 1 [March 9, 2016]


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Electrophoresis News – Subscribe to the Bioanalyzer E-Bulletin

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www.agilent.com/genomics/2100ebulletin

Rev. 1 [March 9, 2016]


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http://www.agilent.com/genomics/2100ebulletin

Agilent 2100 Bioanalyzer Support Tool Box

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www.agilent.com/genomics/bioanalyzer-toolbox

http://www.agilent.com/genomics/bioanalyzer-toolbox



2100 Bioanalyzer Buddy App for iOS and Android smartphones

Tips & Tricks!

Alerts for Expirations -

Chips, Reagents, Gels

Alerts for Maintenance -

Priming Stations & Cartridges

Stopwatches

Priming Complete Alert

Run Complete Alert

Game for lab downtime!

Compete with friends. Can you

move from Dishwasher to

Nobel winner? Download from iTunes and Google Play

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Questions

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bioanalyzer and tapestation summit - agilent · webpage) • vortexer with adaptor • plastic...

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