bioc 300: bioinformatics
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Bioc 300: Bioinformatics. www.geneticsplace.com. Goals of the Course. Understand Methods and Research Questions Analyze Real Data Engage in a Realistic Learning Environment Utilize Online Databases Appreciate Complexity of Research Systems - PowerPoint PPT PresentationTRANSCRIPT
Bioc 300: Bioinformatics
www.geneticsplace.com
Goals of the Course
Understand Methods and Research Questions Analyze Real Data Engage in a Realistic Learning Environment Utilize Online Databases Appreciate Complexity of Research Systems Integrate Different Types of Information Reconsider Cells as Intracellular Ecosystems Integrate Bioinformatics with Biology
What is bioinformatics?
"Bioinformatics is the term coined for the new field that merges biology, computer science, and information technology to manage and analyze the data, with the ultimate goal of understanding and modeling living systems." Genomics and Its Impact on Medicine and Society - A 2001 Primer U.S. Department of Energy Human Genome Program
Bioinformatics also represents a paradigm shift for molecular biology, instead of taking a reductionist approach, the sub-disciplines of bioinformatics are more expansionist: they attempt to study the entire complement of a particular cellular molecule or process.
The “omics” revolution Genomics: The study of the entire DNA complement of an organism
Genome Sequence Information
Applied Research
Basic Research
•Acquiring Sequence•Human Genome Draft•Evolution
•Identification of Biological Unknowns•Biomedical Research
Genomic Variations
Ethics
Human Variations
Ecology•Tracking Ivory Sales•Diatoms and Global Warming
•SNPs•Disease Analysis
•GMO’s•Genetic Testing
DNA Microarrays
Applied Research
Basic Research•Introduction to Method•Data Analysis
•Cancer •Pharmacogenomics
Proteomics
The “omics” revolution Genomics:
The study of the entire DNA complement of an organism
The study of the entire set of proteins in a particular cell type
Proteomics
Identification and Quantification
Protein-Protein Interactions
Cellular Roles
permission form Stan Fields
permission form Stan Fields
permission from Benno Schwikowski
Transcriptonomics
Proteomics
The “omics” revolution Genomics:
The study of the entire DNA complement of an organism
The study of the entire set of proteins in a particular cell type
The study of all mRNA transcripts in a particular cell type Metabolomics
The study of all metabolites in a particular cell type Glycomics
The study of all polysaccharides in a particular cell type Variomics
The study of all possible drug targets in a particular cell type
Genomic Circuits
Integrated Circuits
Toggle Switches
Single Gene Circuit
www.bio.davidson.edu/courses/genomics/circuits.html
Sequencing of Whole Genomes
Three Phases of Genome Sequencing:
Preliminary sequencing Finishing Annotating
Preliminary sequencing 1970’s
Maxam-Gilbert sequencing (chemical cleavage)
Sanger sequencing (dideoxy method)
You could sequence 100’s of bases per day!
Autorad
Genomics “took off” with automated sequencing 1990’s
Leroy Hood made modifications to dideoxy sequencing:
ddNTPs were coupled to fluorescent dyes (instead of radioactivity)
DNA fragments were separated via capillary gel electrophoresis
Sequence read by lasers, data was directly recorded into computer
Now, instead of an autorad, we have a:
Chromat!
The newest DNA sequencers can determine millions of bases of sequence in a day!
The increasing ease of obtaining sequence data has lead to a logarithmic growth of Genbank, the main repository of sequence data which is housed at the National Library of Medicine at NIH.
Growth of Genbank
Sequencing Entire OrganismsBefore the 1990’s, sequencing was somewhat haphazard. Depending on the researcher, different pieces of different organisms’ genomes had been sequenced.No concerted effort had been made to sequence the entire genome of an organism.
HUGO changed all of that, it’s mission was to sequence the human genome, as well as a number of the genomes of model organisms.While small genomes could be sequenced directly, larger genomes were first mapped out.
Mapping large genomesSequencers needed some reference sequences to know what part of a genome they were dealing with.
STSs - sequence tagged sitesThese are defined by a pair of PCR primers that amplify only one segment of a genome (ie. unique sequence).ESTs- expressed sequence tagsThese are short sequences of cDNA that indicate where genes are located within the genome.
Now genomes could be cut into pieces, sequenced, and the pieces reassembled.
Cutting up genomesVectors designed to carry large pieces of DNA include:
BACs- bacterial artificial chromosomes- can carry about 150 kb of insert
YACs- yeast artificial chromosomes- can carry up to 1.5 Mb of insertBACs or YACs containing overlapping DNA can be assembled into contigous overlapping fragments.
“Shotgun” sequencing
While HUGO was busy mapping large genomes and sequencing some small genomes, Craig Venter founded TIGR.TIGR took a completely different approach. Instead of mapping a genome, they simply cut it into thousands of pieces, sequenced the pieces, and reassembled the data using overlapping fragments.It was TIGR, not HUGO, who produced the world’s 1st completed genome in 1995- H. influenzae.
Finishing a Genomic Sequence
A “finished” sequence is defined as one that contains no more than 1 error in 10,000 bases.
Finishing a sequence involves aligning a number of preliminary sequences and correcting any inconsistencies.
Overlapping segments are combined into larger assemblies of contiguous DNA (contigs).
If contigs do not overlap, a gap remains in the sequence.
Finishing continued
The human “draft” sequence, published in 2001, contained 147,821 gaps.
The “finished” sequence, published in 2004, contained 341 gaps.
A gap usually contains highly repetitive DNA that complicates attempts to clone and sequence it.
Finishing is a very expensive process, many genomes have not been finished.
Annotating Genomes
Annotation involves the identification of functionally important sections of a genome.
This includes, but is not limited to, making an educated guess about what kind of protein is encoded by a given coding sequence.
Annotation is performed using various computer programs.
Locating genes within a genome
Prokaryotes contain ORFs with no introns and very little intergenic sequence.
Eukaryotes contain introns, complex promoters, and enhancers
Introns range between 70 and 30,000 bpOne eukaryotic gene can encode more that one different
protein via alternate splicing mechanismsEukaryotes also contain pseudogenes, ORFs which have
been rendered nonfunctional by mutationMammalian genomes contain about 23% pseudogenes
Process is different in prokaryotes vs. eukaryotes
Tools for gene hunting GeneMark - originally created for prokaryotes but
adapted for some model eukaryotes GenScan - accepts up to 1 million bp of sequence
online, more if downloaded Glimmer & GlimmerM - developed by TIGR,
accepts up to 200 kb online, more if downloaded
Once a genome is annotated… One can use a genome browser to locate specific
loci on specific chromosomes One can then use resources such as GeneCard to
find out more about a specific gene
Progress of Genome SequencingSequenced Euk. Genomes Yeast Drosophila C. elegans Arabidopsis Mosquito Human Mouse Rat Chicken Dog Zebra fish
Euk. Genomes in Progress Xenopus Cow Cat Horse Kangaroo Honey Bee Turkey Lobster Bat Hedgehog
and others…
Genomic Search Engines include: BLAST- searches sequence information, either
nucleotide (BLASTn) or protein (BLASTp) BLAST2- aligns two sequences, checking similarity Enterez- searches databases for textual information PubMed- searches scientific literature for text ORF finder- finds Open Reading Frames (genes) PREDATOR- predicts secondary structure of proteins ExPASy- analysis of protein sequence and structure as
well as 2D gel information
Tools had to be developed to make sense of the dearth of genomic data being produced
Calculating E(expect)-values
E-values measure the “significance” of a match, the smaller E-value, the better
E-values are calculated using:1) S, the bit score, a measure of the similarity
between the hit and the query2) m, the length of the query3) n, the size of the database
E = mn2-S
So, how do you get the bit score?
S is calculated from the raw score, R
R = aI + bX - cO - dG
Where I is the # of identities, X is the # of mis-matched nucleotides, O is the # of gaps, and G is the # of spaces in the gap.a, b, c, and d are the rewards, and penalties, for each of these variables.The defaults of these lower-case letters are set at 1, -3, 5, and 2, respectively.
These values can be changed on the “Other advanced” line.
Now that we have a raw score, the bit score can be obtained by normalizing the data:
S = (R - ln K)/ln 2
(where and K are the normalizing parameters)
These parameters are printed at the bottom of a BLAST report.
Normalization enables a direct comparison of E-values and bit scores, even if the reward and penalty variables have been changed by the user.
More databases of interest:
SwissProt- protein sequence database PDB- contains protein structural information OMIM- catalogs human disease genes TIGR- many searchable genomes, esp. bacterial ones GeneCard- genomic, proteomic and phenotypic info. Unigene- catalogs human ESTs Human map viewer- shows chromosomal location of genes
Protein structure and functionFor most researchers, the final goal of genomic research is not the genomic data itself but an understanding of the proteins encoded for by a genome.
Steps to determining protein structure and function: Find ORFs, or coding sequences (CDSs) Translate ORFs
Predict hydropathy using a Kyte-Doolitle plot Check if 3D structure has been determined
Predict secondary structure of your protein
Is this a known protein? If not, find protein orthologs, similar proteins in different species
What do we mean by function?
Why = biological process. The objective toward which this protein contributes.
What = molecular function. The biochemical activity that the protein accomplishes.
Where = cellular component. The location of protein activity.
The term “function” is too simplistic and is somewhat outdated. A consortium called “Gene Ontology” decided that a complete description of function must include not only “why?” but also “what?” and “where?”
One example: isocitrate dehydrogenase (IDH)
OMIM - IDH3A COG - functional categories, dendograms,isoforms- distinct genes encoding similar proteins Enzyme Commission, “EC” numbers Swiss-Prot Phylogenetic trees rooted vs. unrooted
Terms used to describe phylogeny paralogs - genes which arose from a common ancestral
gene within one species (isoforms) orthologs - genes from two organisms which arose
from a common ancestral gene synteny -genetic loci located on the same chromosome
(or multiple genetic loci from different species which are located on a chromosomal region of common ancestry)
homology - sequences which are similar due to a common evolutionary origin
similarity or identity
- terms used to describe sequences without regard to evolutionary relationships
Searching for related proteins
PSI-BLAST allows one to search outward in a spiraling pattern from a central starting point.
First iteration- finds proteins with similar sequences.
Second iteration- can be performed using a consensus sequence computed from your first iteration. More iterations can be performed as desired. Or, one can choose a species and perform another first iteration using the results of the original search.
This approach can be used to annotate ORFs from a newly sequenced genome
Alternate Splicing
60% of human genes produce more than 1 mRNA
Only about 22% of genes in C. elegans fit into this category
Epigenetic ControlIt is not just the coding regions which matter.
Methylation, such as that found in heterochromatin
and CpG islands, also plays a role in gene expression.
At any given time, there are 400,000 mC in a given cell. Since there are about 100 different human cell types, this totals 40 million methylation events in our methylome.Nonmammalian animals lack this form of epigenetic control.
The # of CpG islands correlates with the # of genes on a chromosome
CpGs are usually associated with genes
ImprintingAbout 20 mammalian genes are known to be
methylated during gametogenesis in either the parental or maternal copy.
Imprinting may represent a “genetic tug-of-war” between male and female interests.
For example, the insulin-like growth factor 2, Igf2, is expressed only in the paternal allele. Igf2 promotes the growth of the developing embryo.
The expression of its receptor, Igf2r, is controlled by the maternally inherited allele.
Expression of Paternal Allele of Igf2 in embryo and placenta
How does silencing work?
What is the effect a loss of imprinting? Loss of Igf2 imprinting can lead to colorectal
cancer and Beckwith-Wiedemann Syndrome
There is a cluster of CpG islands in an insulator region near Igf2
CTCF is a protein which only binds to unmethylated DNA.
17/20 tumor samples taken from cancer patients were found to be hypermethylated in this region.
What about the rest of our genome? Since only 1-2% of our genome is coding
sequence what does the rest do?
A majority of our DNA is repetitive sequence There are 5 classes of repetitive sequence:1) transposon derived
4) segmental duplications3) simple repeats such as VNTRs2) pseudogenes
5) heterochromatic regions
The first category alone accounts for 45% of our genome!
TransposonsTransposons fall into 4 categories:
1) SINEs, short interspersed elements, such as Alu comprise 13% of our genome
These may help a cell cope with stress, RNA produced from these bind to an inhibitor of translation.
2) LINEs, long interspersed elements, comprise 21% of our genome
3) LTR retrotransposons comprise 8% of our genome
4) Other DNA transposons 3% of our genome
More Transposon Facts
About 50 genes appear to be derived from transposons, including RAG1 and RAG2, necessary for antibody diversity.
The X chromosome has the highest concentration of transposons- one 525 kb section is 89% transposon-derived.The Y chromosome has the highest concentration of LINEs, it is the most gene-poor of the chromosomes and probably tolerates insertions well.