biochemistry lab1
DESCRIPTION
Johns HOpkinsTRANSCRIPT
Experiment 10 7.8
0.1 11.360.2 11.720.3 11.90.4 12.03
Experiment 20 2.84
0.4 3.70.8 4.031.2 4.261.6 4.44
2 4.612.4 4.772.8 4.942.9 4.99
3 5.043.1 5.13.2 5.153.3 5.213.4 5.273.5 5.343.6 5.433.7 5.513.8 5.623.9 5.75
4 5.924.1 6.24.2 6.84.3 10.55
Experiment 30 2
0.4 2.010.8 2.041.2 2.11.6 2.19
2 2.32.4 2.412.8 2.553.2 2.73.6 2.83
4 3.06
0 0.05 0.1 0.15 0.2 0.25 0.3 0.35 0.40
2
4
6
8
10
12
14
pH of Solution vs. Volume of 0.500M KOH added to 20mL of dH2O
Volume of KOH added (mL)
pH
0 0.5 1 1.5 2 2.5 3 3.5 4 4.50
2
4
6
8
10
12
pH of Solution vs. Volume of 0.500M KOH added to 20mL of 0.100M Acetic Acid
Volume of KOH added (mL)
pH
0 2 4 6 8 10 12 140
2
4
6
8
10
12
14
pH of Solution vs. Volume of 0.500M KOH added to 20mL of 0.100M Unknown Amino Acid
Volume of KOH added (mL)
pH
4.4 3.194.8 3.365.2 3.55.6 3.65
6 3.816.4 3.966.8 4.157.2 4.377.6 4.69
8 5.798.4 8.638.8 9.059.2 9.289.6 9.510 9.67
10.4 9.8210.8 9.9811.2 10.1611.6 10.36
12 10.6312.4 11.0212.8 11.5713.2 11.9313.6 12.12
KOH Volume (mL) pH Observed [HA] [A-] pK' calculated0.4 3.7 0.088235 0.009804 4.6542430.8 4.03 0.076923 0.019231 4.632061.2 4.26 0.066038 0.028302 4.6279771.6 4.44 0.055556 0.037037 4.616091
2 4.61 0.045455 0.045455 4.612.4 4.77 0.035714 0.053571 4.5939092.8 4.94 0.026316 0.061404 4.5720233.2 5.15 0.017241 0.068966 4.547943.6 5.43 0.008475 0.076271 4.475757
4.5922220.054237
0 2 4 6 8 10 12 140
2
4
6
8
10
12
14
pH of Solution vs. Volume of 0.500M KOH added to 20mL of 0.100M Unknown Amino Acid
Volume of KOH added (mL)
pH
0 0.05 0.1 0.15 0.2 0.25 0.3 0.35 0.40
2
4
6
8
10
12
14
pH of Solution vs. Volume of 0.500M KOH added to 20mL of dH2O
Volume of KOH added (mL)
pH
0 0.5 1 1.5 2 2.5 3 3.5 4 4.50
2
4
6
8
10
12
pH of Solution vs. Volume of 0.500M KOH added to 20mL of 0.100M Acetic Acid
Volume of KOH added (mL)
pH
0 2 4 6 8 10 12 140
2
4
6
8
10
12
14
pH of Solution vs. Volume of 0.500M KOH added to 20mL of 0.100M Unknown Amino Acid
Volume of KOH added (mL)
pH
0 2 4 6 8 10 12 140
2
4
6
8
10
12
14
pH of Solution vs. Volume of 0.500M KOH added to 20mL of 0.100M Unknown Amino Acid
Volume of KOH added (mL)
pH