biochemistry lecture 1. blooms taxonomy richard c. overbaugh, lynn schultz old dominion university
TRANSCRIPT
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Biochemistry
Lecture 1
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Bloom’s Taxonomy
Richard C. Overbaugh, Lynn SchultzOld Dominion University
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Student Objectives for this course
• Calculate bioenergetic parameters and evaluate carbon molecules reactions
• Reproduce and explain key metabolic processes: glycolysis, TCA cycle, electron transport and oxidative phosphorylation, fatty acid oxidation
• Analyze protein structure and function and evaluate different methods used to assess and test structure and function
• Trace key metabolites through key pathways, design experiments to test carbon flux
• Compare and contrast modes of metabolism regulation and judge the effects of different modes
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Biochemistry is the chemistry of
Living Systems• The Chemistry of Carbon and Water• Themes for this course:
– The transformation of energy– Levels of complexity
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Cells
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Cells
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Levels of Complexity
Lipids
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The Inner Life of the Cell
• http://multimedia.mcb.harvard.edu/anim_innerlife.html
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Why CarbonBond Strength
(kJ/mol)
C – C 347 – 356
C = C 611
C = C 837
C – O 336
C – H 356 – 460
Si – Si 230
Si – O 368
O – O 146
O = O 498
N – N 163
N = N 418
N = N 946
What can we learn from this?
• C – C bond is stronger than C – O– Stable in oxygen rich
environment!
• Two C – C bonds are stronger than one C = C– Chains are stable!
• C – H bond is strong– Hydrocarbons stable at
room temperature!
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Important Functional Groups
• Alcohol• Thiol• Amine• Ether• Thioether• Peroxide• Disulfide• Aldehyde• Ketone
• Carboxylic acid• Ester• Anhydride• Amide• Thioester• Phosphate• Phosphoester• Phosphoanhydride
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Carbon and Functional Groups
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Other biomolecules
NADP+
PEP
Phosphatidylcholine
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Bioenergetics
CellReactants Products
Steady State = constant flux
STHG
QRTGG ln'0
Structural differences between reactants and products
Concentration differences between reactants and products
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Water
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Hydrogen Bonds
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Water
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Colligative Properties
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pH
pH = -log[H+]
214- M101]OH][H[ wK
14]OHlog[]Hlog[ -
14pOHpH
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Acids
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Buffers
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HA][
]A][H[ -
aKHA H+ + A-
HA][
]A[logppH
-
aK
Hendeson Hasselbalch Equation
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Summary
• Biochemistry is the chemistry of living things– Which is the chemistry of carbon and
water
• Carbons unique bonding properties• Water: hydrogen bonds and
ionization• Buffers and pH