bioelectric communication between neurons
TRANSCRIPT
![Page 1: Bioelectric Communication Between Neurons](https://reader034.vdocument.in/reader034/viewer/2022042701/55ad231c1a28abee0c8b4775/html5/thumbnails/1.jpg)
Ana Margarida Araújo
Ana Rita Oliveira
Felicia Negru
Filipa Leal
Luís Rita
Professora Ana Sebastião 1
![Page 2: Bioelectric Communication Between Neurons](https://reader034.vdocument.in/reader034/viewer/2022042701/55ad231c1a28abee0c8b4775/html5/thumbnails/2.jpg)
Nervous Impulse Transmission
![Page 3: Bioelectric Communication Between Neurons](https://reader034.vdocument.in/reader034/viewer/2022042701/55ad231c1a28abee0c8b4775/html5/thumbnails/3.jpg)
3
![Page 4: Bioelectric Communication Between Neurons](https://reader034.vdocument.in/reader034/viewer/2022042701/55ad231c1a28abee0c8b4775/html5/thumbnails/4.jpg)
4
![Page 5: Bioelectric Communication Between Neurons](https://reader034.vdocument.in/reader034/viewer/2022042701/55ad231c1a28abee0c8b4775/html5/thumbnails/5.jpg)
5
![Page 6: Bioelectric Communication Between Neurons](https://reader034.vdocument.in/reader034/viewer/2022042701/55ad231c1a28abee0c8b4775/html5/thumbnails/6.jpg)
Synapse
![Page 7: Bioelectric Communication Between Neurons](https://reader034.vdocument.in/reader034/viewer/2022042701/55ad231c1a28abee0c8b4775/html5/thumbnails/7.jpg)
Electric
Chemical
7
![Page 8: Bioelectric Communication Between Neurons](https://reader034.vdocument.in/reader034/viewer/2022042701/55ad231c1a28abee0c8b4775/html5/thumbnails/8.jpg)
Chemical Synapses
Action Potential is transmitted by
special proteins called
neurotransmitters.
8
![Page 9: Bioelectric Communication Between Neurons](https://reader034.vdocument.in/reader034/viewer/2022042701/55ad231c1a28abee0c8b4775/html5/thumbnails/9.jpg)
Neurotransmitters Releasing
Neurotransmitters get out from the
pre synaptic cell by exocytosis.
After that they get in the synaptic
cleft.
9
![Page 10: Bioelectric Communication Between Neurons](https://reader034.vdocument.in/reader034/viewer/2022042701/55ad231c1a28abee0c8b4775/html5/thumbnails/10.jpg)
Receptor Molecules In Synapses
After that neurotransmitters
interact with the next cell. This way
the nervous impulse is transmitted
between 2 neurons.
10
![Page 11: Bioelectric Communication Between Neurons](https://reader034.vdocument.in/reader034/viewer/2022042701/55ad231c1a28abee0c8b4775/html5/thumbnails/11.jpg)
GABBA
11
Glutamate
Inhibitory Responses Excitatory Responses
![Page 12: Bioelectric Communication Between Neurons](https://reader034.vdocument.in/reader034/viewer/2022042701/55ad231c1a28abee0c8b4775/html5/thumbnails/12.jpg)
o Exocytose vesicles, spontaneously,
merge with the pre synaptic
membrane, propagating a nervous
impulse to the next neuron.
12
![Page 13: Bioelectric Communication Between Neurons](https://reader034.vdocument.in/reader034/viewer/2022042701/55ad231c1a28abee0c8b4775/html5/thumbnails/13.jpg)
o Substance that alters
nervous impulse
transmission.
13
Adenosine
![Page 14: Bioelectric Communication Between Neurons](https://reader034.vdocument.in/reader034/viewer/2022042701/55ad231c1a28abee0c8b4775/html5/thumbnails/14.jpg)
o It is a secreted protein
in humans.
o Encourage the growth
and differentiation of
new neurons.
o BDNF itself is
important for long-
term memory.
14
![Page 15: Bioelectric Communication Between Neurons](https://reader034.vdocument.in/reader034/viewer/2022042701/55ad231c1a28abee0c8b4775/html5/thumbnails/15.jpg)
o It plays an important role
in the consolidation of
information from short to
long-term memory.
15
![Page 16: Bioelectric Communication Between Neurons](https://reader034.vdocument.in/reader034/viewer/2022042701/55ad231c1a28abee0c8b4775/html5/thumbnails/16.jpg)
The goal of this experiment was to address weather BDNF
effects on mIPSC frequency are dependent on A2ARs
activation.
16
![Page 17: Bioelectric Communication Between Neurons](https://reader034.vdocument.in/reader034/viewer/2022042701/55ad231c1a28abee0c8b4775/html5/thumbnails/17.jpg)
Patch Clamp Method
![Page 18: Bioelectric Communication Between Neurons](https://reader034.vdocument.in/reader034/viewer/2022042701/55ad231c1a28abee0c8b4775/html5/thumbnails/18.jpg)
Loose Patch
Cell Atatched
Whole Cell
Outside Out
Patch
Inside Out Patch
18
![Page 19: Bioelectric Communication Between Neurons](https://reader034.vdocument.in/reader034/viewer/2022042701/55ad231c1a28abee0c8b4775/html5/thumbnails/19.jpg)
19
o Removal of the
brain
o Dissection of the
hippocampus
o Acute brain slices
technique
o 300µm slices
o 2 to 4 weeks old
o Anesthetization
o Decapitation
![Page 20: Bioelectric Communication Between Neurons](https://reader034.vdocument.in/reader034/viewer/2022042701/55ad231c1a28abee0c8b4775/html5/thumbnails/20.jpg)
Vibratom Hipppocampal Slice
20
![Page 21: Bioelectric Communication Between Neurons](https://reader034.vdocument.in/reader034/viewer/2022042701/55ad231c1a28abee0c8b4775/html5/thumbnails/21.jpg)
Visibility in
different
plans
Triangularly shaped
Not too bright
21
![Page 22: Bioelectric Communication Between Neurons](https://reader034.vdocument.in/reader034/viewer/2022042701/55ad231c1a28abee0c8b4775/html5/thumbnails/22.jpg)
Videos youtube
22
![Page 23: Bioelectric Communication Between Neurons](https://reader034.vdocument.in/reader034/viewer/2022042701/55ad231c1a28abee0c8b4775/html5/thumbnails/23.jpg)
http://www.youtube.com/watch?v=xwqFdL_ndMo http://www.youtube.com/watch?v=5ZgiacYwkyY
23
![Page 24: Bioelectric Communication Between Neurons](https://reader034.vdocument.in/reader034/viewer/2022042701/55ad231c1a28abee0c8b4775/html5/thumbnails/24.jpg)
Whole Cell Patch Clamp 24
![Page 25: Bioelectric Communication Between Neurons](https://reader034.vdocument.in/reader034/viewer/2022042701/55ad231c1a28abee0c8b4775/html5/thumbnails/25.jpg)
Miniature events of the inhibitory response
25
![Page 26: Bioelectric Communication Between Neurons](https://reader034.vdocument.in/reader034/viewer/2022042701/55ad231c1a28abee0c8b4775/html5/thumbnails/26.jpg)
Adenosine A2A receptor agonist
Adenosine Activates Track B
receptors Potentiates
Effects of BDNF
26
![Page 27: Bioelectric Communication Between Neurons](https://reader034.vdocument.in/reader034/viewer/2022042701/55ad231c1a28abee0c8b4775/html5/thumbnails/27.jpg)
10 minutes of control time - with TTX, KYN, and SCH
40 minutes of data registration of the inhibitory response
10 min || 40 min
TTX, KYN,
SCH (100 nM)
BDNF 10
(ng/mL)
27
![Page 28: Bioelectric Communication Between Neurons](https://reader034.vdocument.in/reader034/viewer/2022042701/55ad231c1a28abee0c8b4775/html5/thumbnails/28.jpg)
28
Inverted Light Microscope
![Page 29: Bioelectric Communication Between Neurons](https://reader034.vdocument.in/reader034/viewer/2022042701/55ad231c1a28abee0c8b4775/html5/thumbnails/29.jpg)
29
Faraday´s Cage
![Page 30: Bioelectric Communication Between Neurons](https://reader034.vdocument.in/reader034/viewer/2022042701/55ad231c1a28abee0c8b4775/html5/thumbnails/30.jpg)
30
Air Table
![Page 31: Bioelectric Communication Between Neurons](https://reader034.vdocument.in/reader034/viewer/2022042701/55ad231c1a28abee0c8b4775/html5/thumbnails/31.jpg)
31
Micromanipulator
![Page 32: Bioelectric Communication Between Neurons](https://reader034.vdocument.in/reader034/viewer/2022042701/55ad231c1a28abee0c8b4775/html5/thumbnails/32.jpg)
32
Recording Electrode
![Page 33: Bioelectric Communication Between Neurons](https://reader034.vdocument.in/reader034/viewer/2022042701/55ad231c1a28abee0c8b4775/html5/thumbnails/33.jpg)
33
![Page 34: Bioelectric Communication Between Neurons](https://reader034.vdocument.in/reader034/viewer/2022042701/55ad231c1a28abee0c8b4775/html5/thumbnails/34.jpg)
34
![Page 35: Bioelectric Communication Between Neurons](https://reader034.vdocument.in/reader034/viewer/2022042701/55ad231c1a28abee0c8b4775/html5/thumbnails/35.jpg)
0 20 40
0
50
100
150
Time (min)
mIP
SC
fre
qu
en
cy
(% b
ase
line
)
BDNF 10 ng/mL
SCH 100 nM
0 20 40
0
50
100
150
Time (min)
mIP
SC
am
plit
ud
e
(% b
ase
line
)
BDNF 10 ng/mL
SCH 100 nM
35
![Page 36: Bioelectric Communication Between Neurons](https://reader034.vdocument.in/reader034/viewer/2022042701/55ad231c1a28abee0c8b4775/html5/thumbnails/36.jpg)
o Adition of SCH prevents the effects of BDNF;
o Results from a single experimente;
o Plenty of room for Biomedical Engeneering to
improve essencial equipement.
36
![Page 37: Bioelectric Communication Between Neurons](https://reader034.vdocument.in/reader034/viewer/2022042701/55ad231c1a28abee0c8b4775/html5/thumbnails/37.jpg)
37
1. http://www.bem.fi/book/04/04x/0427x.htm SLIDE 19
2. http://www.leicabiosystems.com/specimen-preparation/sectioning/vibrating-blade-
microtomes/details/product/leica-vt1000-a/ SLIDE 21
3. http://www.physiologie.uni-freiburg.de/research-techniques/electrophysiology
SLIDE 22
4. http://www.physiologie.uni-freiburg.de/research-techniques/electrophysiology
SLIDE 25