Hacking My GutMicrobiome

Richard SpragueJune 4, 2016

I’m not a doctor• Disclaimer• The following presentation and discussion is not intended to diagnose, treat, cure, or prevent any

disease. • The material in my talk is provided for informational purposes only and is not medical advice. • Always consult your physician before beginning any diet or exercise program.

• uBiome• uBiome sampling kits and data is designed to be a tool for personal research - not a diagnostic

tool. uBiome cannot predict the implications of your results for your long-term health, nor will you be able to use your results to diagnose, treat, cure, or prevent any medical condition.

• uBiome offers the opportunity to gain a better understanding of the human microbiome and see the bacteria in your own samples.

• uBiome’s platform enables you to visualize your results, as they compare to other participant groups and your own samples over time.

YouWeight

Human Non-Human

Cells

Human Non-Human

Genes

Human Non-Human

YouWeight

Human Non-Human

Cells

Human Non-Human

Genes

Human Non-Human

3 pounds

Find which microbes you have

{ "ubiome_bacteriacounts": [ { "count": 611672, "count_norm": 1008824, "parent": 0, "tax_rank": "root", "tax_color": null, "avg": null, "taxon": 1, "tax_name": "root" },

{ "count": 606322, "count_norm": 1000000, "parent": 131567, "tax_rank": "superkingdom", "tax_color": null, "avg": null, "taxon": 2, "tax_name": "Bacteria“},

{ "ubiome_bacteriacounts": [ { "count": 611672, "count_norm": 1008824, "parent": 0, "tax_rank": "root", "tax_color": null, "avg": null, "taxon": 1, "tax_name": "root" },

tax_name tax_rank May-14 Jun-14 Oct-14 Jan-15 Feb-15 21-Apr 28-Apr Jun-15 Aug-15

Firmicutes phylum 62.288% 46.338% 58.450% 71.836% 67.930% 79.980% 68.885% 67.761% 63.708%

Bacteroidetes phylum 25.573% 29.927% 27.485% 16.424% 14.900% 9.684% 13.602% 21.760% 18.289%

Proteobacteria phylum 2.976% 15.625% 0.359% 0.982% 7.514% 1.584% 3.853% 2.111% 1.280%Actinobacteria phylum 1.097% 0.832% 6.562% 7.616% 0.771% 1.041% 0.382% 1.098% 0.967%Tenericutes phylum 0.636% 0.249% 0.043% 0.065% 0.071% 0.098% 0.647% 0.530% 0.003%Verrucomicrobia phylum 3.135% 1.965% 0.770% 0.664% 0.013% 1.155% 0.957% 0.236% 4.418%Euryarchaeota phylum 0.000% 0.000% 0.000% 0.000% 0.000% 2.425% 0.227% 0.225% 0.637%Lentisphaerae phylum 0.170% 0.000% 0.118% 0.017% 0.009% 0.018% 0.024% 0.107% 0.108%Cyanobacteria phylum 0.001% 0.000% 0.003% 0.005% 0.031% 0.005% 0.000% 0.096% 0.012%

{ "count": 606322, "count_norm": 1000000, "parent": 131567, "tax_rank": "superkingdom", "tax_color": null, "avg": null, "taxon": 2, "tax_name": "Bacteria“},

May-14

Jun-14

Oct-14

Jan-15

Feb-15

21-Apr

28-Apr

Jun-15

Aug-15

0%20%40%60%80%

100%Phylum Composition

EuryarchaeotaVerrucomicrobiaTenericutesActinobacteriaProteobacteriaBacteroidetesFirmicutes

Making science news personal

Bacteroides Plebeius

Hacking my microbiome

1 2 3 4 50

10000

20000

30000

40000

50000

60000

70000

Bifidobacterium

Sample

Coun

t (n

orm

aliz

ed)

Start Stop

Microbiome and sleep

ReseedingDate Calories Carbs Fat Protein

Cholesterol Sodium Sugars Fibre

15-Oct-15 2542 332 79 104 165 3339 53 48

17-Oct-15 1290 194 32 38 76 2907 58 19

18-Oct-15 0 0 0 0 0 0 0 0

19-Oct-15 0 0 0 0 0 0 0 0

20-Oct-15 2644 292 119 102 557 3266 61 41

21-Oct-15 2497 234 98 103 422 2593 89 44

22-Oct-15 2017 280 43 62 110 1577 58 29

23-Oct-15 2674 369 78 122 75 1305 49 36

24-Oct-15 2238 182 82 174 365 3123 62 30

25-Oct-15 1582 160 48 110 239 3084 38 19

26-Oct-15 1876 226 63 73 186 920 52 33

27-Oct-15 1771 173 62 97 767 1301 33 22

28-Oct-15 2190 291 88 100 252 1043 65 32

29-Oct-15 1768 161 88 90 267 1664 54 22

30-Oct-15 938 107 37 43 114 781 34 10

31-Oct-15 1637 251 42 94 223 4184 17 11

1-Nov-15 2133 198 69 100 380 1953 53 9

2-Nov-15 2142 269 76 60 306 2193 46 30

3-Nov-15 2019 300 97 70 255 2532 160 34

Cleanse

My new friends

Abiotrophia Bacillus Catonella

Christensenella Parvimonas

What I Learned• My gut microbiome recovers quickly• Amounts and ratios change, but not the

organisms• A few intriguing, new organisms

appeared.• It’s hard to significantly change your

microbiome

Thank you!

• http://ubiome.com

@sprague