GENETIC VARIATIONS BETWEEN HAEMOGLOBIN PROTEIN OF HUMAN AND MACACA FASCICULARIS.…..

BY_ANIKET BAGUL

OUTLINE INTRODUCTION DATABASE OF HAEMOGLOBIN PROTEIN FASTA FORMAT SEQUENCE ALIGNMENT GRAPHICAL DISPLAY ALIGNMENT DESCRIPTION PHYLOGENETIC TREES AMINO ACID ANNOTATIONS AND PROPERTIES MAP VIEWER SEQUENCE VIEWER EPIGENOMICS 3D STRUCTURE OF PROTEIN CONCLUSION REFERENCE

DATABASE IN BIOINFORMETICS

NUCLEOTIDE SEQUENCE

PROTEIN SEQUENCE

3D STRUCTURE OF MOLECULE

ALIGNMENT

GENE EXPRESSION

GRAPHICAL DISPLAY

EPIGENOMICS

BIOINFORMETICS-Application of computer science that deals with the study of molecular biology

It deals with coding and decoding of sequence of nucleotides

Branches- ‘Genome , ‘Genomics , ‘Proteome , ‘Proteomics .

Central dogma….DNA-RNA-PROTEIN

HAEMOGLOBIN PROTEIN

HAEMOGLOBIN (HB) IS OXYGEN BINDING PROTEIN

IT CONTAINS 2 PARTS – HEME LIGAND & GLOBIN PROTEIN PART

HEME PART IS SYNTHESIZED IN MITOCHONDRIA AND IMMATURE RBC’s

GLOBIN PROTEIN PART IS SYNTHESIZED BY RIBOSOMES IN CYOTOSOL

DATABASE OF HUMAN HB PROTEIN

DATABASE OF MACACA HB PROTEIN

FASTA FORMAT GTGCACCTGACTCCTGAGGAGAAGTCTGCC

GTTACTGCCCTGTGGGGCAAGGTGAACGTGGATGAAGTTG GTGGTGAGGCCCTGGGCAGGCTGCTGGTGGTCTACCCTTGGACCCAGAGGTTCTTTGAGTCCTTTGGGGA TCTGTCCACTCCTGATGCTGTTATGGGCAACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCC TTTAGTGATGGCCTGGCTCACCTGGACAACCTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTG ACAAGCTGCACGTGGATCCTGAGAACTTCAGGCTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATCA CTTTGGCAAAGAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCC CTGGCCCACAAGTATCAC

ACACTTGCTTCTGACACAACTGTGTTCACGAGCAACCTCAAACAGACACCATGGTGCATCTGACTCCTGA GGAGAAGAATGCCGTCACCACCCTGTGGGGCAAGGTGAACGTGGATGAAGTTGGTGGTGAGGCCCTGGGC AGGCTGCTGGTGGTCTACCCTTGGACCCAGAGGTTCTTTGAGTCCTTTGGGGATCTGTCCTCTCCTGATG CTGTTATGGGCAACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTTGGTGCCTTTAGTGATGGCCTGAA TCACCTGGACAACCTCAAGGGTACCTTTGCCCAGCTCAGTGAGCTGCACTGTGACAAGCTGCATGTGGAT CCTGAGAACTTCAAGCTCCTGGGCAACGTGCTGGTGTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCA CCCCGCAAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTACCA CTAAGCTCACTTTCTTGCTGTCCAATTTCTACCAAAGGTTCCTTTGTTCCCAAAGTCCAACTACTGAACT GGGGGATATTATGAAGGGCCTTGAGGATCTGGATTCTGCCTAAT

HUMAN HB PROTEIN MACACA HB PROTEIN

SEQUENCE ALIGNMENT

GRAPHICAL DISPLAY

ALIGNMENT DESCRIPTION

PHYLOGENETIC TREE OF HUMAN HB

PHYLOGENETIC TREE OF MACACA HB

ALIGNMENT ANNOTATIONS AND AMINO ACID PROPERTIES

AMINO ACID PROPERTIES

AMINO ACID PROPERTIES

GENE – MAP VIEWER OF HUMAN HB

GENE POSITION AND SIZE

SEQUENCE VIEWER

GENE – MAP VIEWER OF MACACA HB

GENE POSITION AND SIZE

SEQUENCE VIEWER

EPIGENOMICS OF HB PROTEIN

HUMAN HB PROTEIN 3D STRUCTURE

MACACA HB PROTEIN 3D STRUCTURE

HEME PART OF HAEMOGLOBIN

CONCLUSION THE SEQUENCE ALIGNMENT SHOWS MORE THAN 200

SCORE THE IDENTITIES OBSERVED – 419/439…. ABOUT 95% PHYLOGENETIC TREE SHOWS ABOUT 0.85 SEQUENCE

DIFFERENCE IN HUMANS HB SYNTHESIZING GENE IS LOCATED ON

11TH CHROMOSOME AND IN MACACA FASCICULARIS ITS ON 14TH CHROMOSOME

SIZE OF GENE IS ABOUT 1,606BP IN HUMAN AND 1,853BP IN MACACA FASCICULARIS

HEME PART IS PERMANENTLY ATTACHED IN HUMAN HB PROTEIN BUT IN MACAC FASCICULARIS DUE TO ITS PRIMARY STRUCTURE IT IS ATTACHED TEMPARORYLY ONLY DURING OXYGEN TRANSPORTATION

REFERENCE