biological membranes penghui lin nov. 15. 2013. membranes inquiryb/webquest/fa06/mvogenbe
TRANSCRIPT
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Biological Membranes
Penghui LinNov. 15. 2013
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Membranes
http://www.uvm.edu/~inquiryb/webquest/fa06/mvogenbe/
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Diffusion across plasma membrane
http://en.wikipedia.org/wiki/Cell_membrane
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Plasma Membrane
Pabst G. Applications of neutron and X-ray scattering to the study of biologically relevant model membranes J.Chem Phys Lipids. 2010 Jun;163(6):460-79
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Man-made membrane
http://bioweb.wku.edu/courses/biol115/wyatt/biochem/lipid/Lipid_2.asp
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Liquid crystals
• Nematic phase
• Smectic phases
• Chiral phases
http://en.wikipedia.org/wiki/Liquid_crystal
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Sample preparation
Jörg Fitter, Thomas Gutberlet, John Katsaras. Neutron Scattering in Biology, Techniques and Applications. 2006 ISBN: 978-3-540-29108-4 (Print) 978-3-540-29111-4 (Online)
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Incident angle = reflected angle
Specular Neutron Reflectivity
Jörg Fitter, Thomas Gutberlet, John Katsaras. Neutron Scattering in Biology, Techniques and Applications. 2006 ISBN: 978-3-540-29108-4 (Print) 978-3-540-29111-4 (Online)
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Density profile ρ0(z)
Fourier Synthesis
Jörg Fitter, Thomas Gutberlet, John Katsaras. Neutron Scattering in Biology, Techniques and Applications. 2006 ISBN: 978-3-540-29108-4 (Print) 978-3-540-29111-4 (Online)
![Page 10: Biological Membranes Penghui Lin Nov. 15. 2013. Membranes inquiryb/webquest/fa06/mvogenbe](https://reader030.vdocument.in/reader030/viewer/2022033106/56649e4c5503460f94b413de/html5/thumbnails/10.jpg)
Lipid bilayer phases
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Location of Cholesterol
Pabst G. Applications of neutron and X-ray scattering to the study of biologically relevant model membranes J.Chem Phys Lipids. 2010 Jun;163(6):460-79
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Location of cholesterol
Pabst G. Applications of neutron and X-ray scattering to the study of biologically relevant model membranes J.Chem Phys Lipids. 2010 Jun;163(6):460-79
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Forces between membranes
Disjoining pressureOsmotic pressure
Pabst G. Applications of neutron and X-ray scattering to the study of biologically relevant model membranes J.Chem Phys Lipids. 2010 Jun;163(6):460-79
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Osmotic stress experiment
Neutron/
Pabst G. Applications of neutron and X-ray scattering to the study of biologically relevant model membranes J.Chem Phys Lipids. 2010 Jun;163(6):460-79
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Thermal unbinding
Pabst G. Applications of neutron and X-ray scattering to the study of biologically relevant model membranes J.Chem Phys Lipids. 2010 Jun;163(6):460-79
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Single component membrane
Pabst G. Applications of neutron and X-ray scattering to the study of biologically relevant model membranes J.Chem Phys Lipids. 2010 Jun;163(6):460-79
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Lipid mixtures and domains
• Ideal mixing: no phase separation
• Non-ideal or non-random mixing: compositional fluctuation happens while no stable domains formed
• Macroscopic phase separation: micron-size stable lipid domains are formed
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No phase separation
Membranethickness
Fluctuationparameter
in DMPC bilayers at 35°Pabst G. Applications of neutron and X-ray scattering to the study of biologically relevant model membranes J.Chem Phys Lipids. 2010 Jun;163(6):460-79
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Macroscopic phase separation
DPPC/DOPC/cholesterol
Pabst G. Applications of neutron and X-ray scattering to the study of biologically relevant model membranes J.Chem Phys Lipids. 2010 Jun;163(6):460-79
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Supramolecular properties
Diffused scattering
Tilted phase
Interdigitated phase
Pabst G. Applications of neutron and X-ray scattering to the study of biologically relevant model membranes J.Chem Phys Lipids. 2010 Jun;163(6):460-79
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Bicelle mixtures
Pabst G. Applications of neutron and X-ray scattering to the study of biologically relevant model membranes J.Chem Phys Lipids. 2010 Jun;163(6):460-79
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Bicelle mixtures
Pabst G. Applications of neutron and X-ray scattering to the study of biologically relevant model membranes J.Chem Phys Lipids. 2010 Jun;163(6):460-79
![Page 23: Biological Membranes Penghui Lin Nov. 15. 2013. Membranes inquiryb/webquest/fa06/mvogenbe](https://reader030.vdocument.in/reader030/viewer/2022033106/56649e4c5503460f94b413de/html5/thumbnails/23.jpg)
Fluctuation change upon antimicrobial peptide binding
Jörg Fitter, Thomas Gutberlet, John Katsaras. Neutron Scattering in Biology, Techniques and Applications. 2006 ISBN: 978-3-540-29108-4 (Print) 978-3-540-29111-4 (Online)
![Page 24: Biological Membranes Penghui Lin Nov. 15. 2013. Membranes inquiryb/webquest/fa06/mvogenbe](https://reader030.vdocument.in/reader030/viewer/2022033106/56649e4c5503460f94b413de/html5/thumbnails/24.jpg)
Membrane rigidity change
Pabst G. Applications of neutron and X-ray scattering to the study of biologically relevant model membranes J.Chem Phys Lipids. 2010 Jun;163(6):460-79
![Page 25: Biological Membranes Penghui Lin Nov. 15. 2013. Membranes inquiryb/webquest/fa06/mvogenbe](https://reader030.vdocument.in/reader030/viewer/2022033106/56649e4c5503460f94b413de/html5/thumbnails/25.jpg)
Pabst G. Applications of neutron and X-ray scattering to the study of biologically relevant model membranes J.Chem Phys Lipids. 2010 Jun;163(6):460-79
![Page 26: Biological Membranes Penghui Lin Nov. 15. 2013. Membranes inquiryb/webquest/fa06/mvogenbe](https://reader030.vdocument.in/reader030/viewer/2022033106/56649e4c5503460f94b413de/html5/thumbnails/26.jpg)
Thanks!
Jörg Fitter, Thomas Gutberlet, John Katsaras. Neutron Scattering in Biology, Techniques and Applications. 2006 ISBN: 978-3-540-29108-4 (Print) 978-3-540-29111-4 (Online)