biology 201
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Biology 201. Introduction to the Microscope. Laboratory Safety and Etiquette. Cabrillo College Department of Biology. Laboratory Rules. No eating or drinking in labs Do not enter halls; remain only in classroom Clean up after yourself. Laboratory Etiquette. - PowerPoint PPT PresentationTRANSCRIPT
Biology 201
Introduction to the Microscope
Laboratory Safety and Etiquette
Cabrillo College Department of Biology
Laboratory Rules
No eating or drinking in labs
Do not enter halls; remain only in classroom
Clean up after yourself
Laboratory Etiquette
Clean your personal workspace after use• Wipe down benches with disinfectant before and after
use
Ensure that no trash is left behind No trash in the sinks Push in chairs Place lab items back on carts and trays
Laboratory Safety Outline
1. Laboratory Hygiene2. Sharps Safety3. Safety Equipment4. Fire Safety5. Chemical Safety6. Biohazard Safety
1. Laboratory Hygiene
Avoid cross contamination• Do not touch self, faucets, doorknobs, notebooks,
pens etc. with gloves on.
Clean and disinfect your workspace
Scrub hands thoroughly before leaving lab
2. Sharps Safety Sharps include
• Razor and scalpel blades• Needles and pins• Microscope cover slips• Broken glass
Place sharps in designated, labeled sharps boxes
Larger broken glass pieces go into designated glass box (ask for help)
NO SHARPS OR GLASS IN THE TRASH
3. Safety Equipment
Shower and eyewash (15 min. soak)• Do not hesitate to use this equipment if necessary
First aid kit
4. Fire Safety Never leave flames unattended Do not use flammables near ignition
sources Fire Extinguishers
• Pull ring, aim at fire’s base, squeeze handle, sweep back and forth (evacuate if >1m3)
Fire Blanket Fire alarm pulls
5. Chemical Safety HAZMAT is short for “Hazardous Material”
~Any material that would be a danger to life or the environment if released without precautions
Dispose of chemical waste in provided containers
DO NOT pour chemical wastes down the drain
Wear gloves and safety glasses when appropriate
Follow the instructor’s directions when dealing with any chemical
6. BIOHAZARD Safety BIOHAZARD is short for Biological Hazard
~Any biological substance or substances that may pose a threat to the health of a living organism, particularly humans
Biohazards include, but are not limited to: Human cells, bodily fluids, and bacterial cultures
Biohazard sharps must be placed into the biohazard Sharps container
Solid Biohazard materials must be sealed in a biohazard bag, and then placed into the biohazard bin for professional disposal
History of the Microscope• Anton van Leeuwenhoek (1676) was first
to see microorganisms
• Robert Hooke proposed “cell theory” and invented primitive compound microscopes
History of the Microscope
A Compound Microscope
Rules for microscope protection1. Cary microscope upright with two hands2. Clean lenses with lens paper ONLY3. Always begin with lowest power objective4. Focus downwards5. Never use course focus with the 10x, 40x
or 100x objective6. When returning to cabinet: remove last
slide, lower stage, put 4x objective in place, turn dimmer down to 1, wrap cord, place in cabinet with eyepieces facing inward
Slides we will look at today
1. Letter “e”2. Colored threads3. Elodea plant4. Cheek cells5. Blood smear
This slide is brought to you by the letter “e” (40x objective)
Slide of thread with 40x objective
Slide of elodea with 10x and 40x objectives
Cytoplasmic streaming of chloroplasts inside elodea
http://www.youtube.com/watch?v=PFtzs_cUddI
Slide of elodea with 40x objective in Sodium chloride
Properties of Light
Properties of Light
• Oil matches the refraction index of glass
Slide of human cheek cells with 40x objective
Slide of human blood cells under oil immersion
(100x objective)
Clean up reminder
The end