biology 402 senior seminar spring 2006. a comparison of bacterial reduction between antiseptics

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Biology 402 Senior Seminar Spring 2006

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Page 1: Biology 402 Senior Seminar Spring 2006. A Comparison of Bacterial Reduction between Antiseptics

Biology 402Senior Seminar

Spring 2006

Page 2: Biology 402 Senior Seminar Spring 2006. A Comparison of Bacterial Reduction between Antiseptics

A Comparison of Bacterial Reduction between

Antiseptics

Page 3: Biology 402 Senior Seminar Spring 2006. A Comparison of Bacterial Reduction between Antiseptics

Studying E. coli strain DHα5

Senior Thesis

Minh Nguyen

Dr. Hartman, Dr. Olney

Saint Martin’s University

Page 4: Biology 402 Senior Seminar Spring 2006. A Comparison of Bacterial Reduction between Antiseptics

Results Of Disinfectant Sprays After 48 Hour Incubation

Clorox Spray Lysol Spray

Safeway Spray Water

Beth Layton

Page 5: Biology 402 Senior Seminar Spring 2006. A Comparison of Bacterial Reduction between Antiseptics

Methods General Daily Routine• Workout Location: Tae Kwon

Do Academy in Puyallup WA.

• Subjects: 14 total volunteers.

• Workout duration: 60 minutes for a 5 day period.

• Hydration: First week-water consumption and the second week, Gatorade consumption.•

• Participants are required to consumed the given beverage.

• Then be weighed • Workout for a 60 minute

period. • At the end of the class, weigh

once more.• Difference in weight will

determine sweat loss.

Kyle Samples

Page 6: Biology 402 Senior Seminar Spring 2006. A Comparison of Bacterial Reduction between Antiseptics

Comparing Black bear damage on conifer trees in Washington

State Capitol Forest

By:Brad Beerbower

Page 7: Biology 402 Senior Seminar Spring 2006. A Comparison of Bacterial Reduction between Antiseptics

Methods

Separate fish randomly in to 3 tanks (each tank with 20 fish)↓

Let fish acclimate to new tank for one day ↓

Weigh food before feeding to the fish. ↓

1 g Feed every 12 hrs for 5 consecutive days↓

Clean the tanks every 24 hrs before feeding them again.↓

Collect unnecessary food and fecal material.

Ruby Lopez

Page 8: Biology 402 Senior Seminar Spring 2006. A Comparison of Bacterial Reduction between Antiseptics

FLESH FLY(Sarcophaga sp.)

• Carrion-feeding flies

• Approx. 2600 known species

• Worldwide

• Decomposition

• Forensics Science – Time of death

http://www.uknature.co.uk/FleshFly-info.htmlSothear Sam

Page 9: Biology 402 Senior Seminar Spring 2006. A Comparison of Bacterial Reduction between Antiseptics

Hypothesis• Those with acrylic nails

will harbor more bacteria underneath their nails than females with native nails (unwashed and washed).

• Image references:http://phuket-beautymassage.com/nailpics.html

http://www.brazilnaturalnails.com/news.html

Pelenita Tuupo

Page 10: Biology 402 Senior Seminar Spring 2006. A Comparison of Bacterial Reduction between Antiseptics

An Investigation of the Amounts of Bacteria on Employees’ HandsSample• 50 total participants

– 40 grocery store employees

– 10 Saint Martin’s students

Occupations• Cashier• Produce/Bakery Clerk• Deli Clerk

• Meat/Seafood Clerk

• Student

Katie Illes

Page 11: Biology 402 Senior Seminar Spring 2006. A Comparison of Bacterial Reduction between Antiseptics

Oral Bacteria

http://www.stchas.edu/faculty/zfitzgerald/lactobacillus.jpg http://www.dokidoki.ne.jp/home2/saishika/mutans1L.jpg

Lactobacillus Streptococcus mutans

•Reside in oral flora

•Adhere to Enamel and Ingest Sugars

•Cause plaque

•Initiate cavitiesChris Leyster

Page 12: Biology 402 Senior Seminar Spring 2006. A Comparison of Bacterial Reduction between Antiseptics

Components of Study

ToothpasteStreptococcus mutans Lactobacillus

Total

24 Hours 72 Hours 24 Hours 72 Hours

Aquafresh 8 8 8 8 32

Arm n’ Hammer 8 8 8 8 32

Crest 8 8 8 8 32

Colgate 8 8 8 8 32

Toms 8 8 8 8 32

Control (Water) 4 4 4 4 16

Total 44 44 44 44 176

Chris Leyster

Page 13: Biology 402 Senior Seminar Spring 2006. A Comparison of Bacterial Reduction between Antiseptics

24 Hour Zones of Inhibition on Lactobacillus

0

5

10

15

20

25

30

crest colgate aquafresh toms arm nhammer

Toothpastes

mm

Chris Leyster

Page 14: Biology 402 Senior Seminar Spring 2006. A Comparison of Bacterial Reduction between Antiseptics

Research Goal

The goal of this project was to ascertain the anti-microbial

power of common varieties of chewing gum. The two varieties tested were Orbit (cinnamon)

that is distributed by Wrigley Co. and Trident (original).

Carter Richards

Page 15: Biology 402 Senior Seminar Spring 2006. A Comparison of Bacterial Reduction between Antiseptics

ResultsFigure 1- Breakfast eaters vs. non-breakfast eaters in a sample of 20 college students. Mean differences between both testing days are

shown in this figure.

30%

50%

70%

90%

1 3 5 7 9 11 13 15 17 19

Student Number

Te

st

Pe

rce

nta

ge

Breakfast No breakfast

Rachael Schroll

Page 16: Biology 402 Senior Seminar Spring 2006. A Comparison of Bacterial Reduction between Antiseptics

Correlation of Coarse Woody Debris

Biomass and Tree Species Diversity within Coniferous

Forests of Western Washington

Rachel Wehr

Page 17: Biology 402 Senior Seminar Spring 2006. A Comparison of Bacterial Reduction between Antiseptics

COARSE WOODY DEBRIS: LOGS

Rachel Wehr

Page 18: Biology 402 Senior Seminar Spring 2006. A Comparison of Bacterial Reduction between Antiseptics

CORRELATION ANALYSIS

0

50

100

150

200

250

0 0.5 1 1.5

diversity index

CW

D b

iom

ass

young forest

mature forest

old growth forest

Correlation coefficients

Young r = -0.207 , P = 0.793

Mature r = -0.628 , P = 0.372

Old Growth r = 0.295 , P = 0.705Rachel Wehr

Page 19: Biology 402 Senior Seminar Spring 2006. A Comparison of Bacterial Reduction between Antiseptics

Oral hygiene habits and oral bacterial populations

A comparison of Lactobacillus and Streptococcus bacteria

Travis Ochsner

4/21/2006

Senior Seminar

Dr. Olney

Dr. Hartman

Page 20: Biology 402 Senior Seminar Spring 2006. A Comparison of Bacterial Reduction between Antiseptics

Hypothesis

• Excessive oral hygiene habits will decrease populations of Lactobacillus bacteria (Tomato juice broth pH 4) more drastically than Streptococcus bacteria (Brain-Heart infusion {BHI} broth pH 6.5).

• Factors- hygiene, bacterial growth, and interaction.

Travis Ochsner

Page 21: Biology 402 Senior Seminar Spring 2006. A Comparison of Bacterial Reduction between Antiseptics

Senior Seminar Project

Chagas Disease

Sara Trejo

Dr. Margaret Olney and Dr. Mary Jo Hartman

Saint Martin’s University 2006

Page 22: Biology 402 Senior Seminar Spring 2006. A Comparison of Bacterial Reduction between Antiseptics

Hypothesis Statement• Polymerase Chain Reaction (PCR) can be developed for use as a

specific and sensitive diagnostic tool for Chagas Disease.

• First step towards development is the evaluation of PCR primer sets for specificity and sensitivity.

Sara Trejo

Page 23: Biology 402 Senior Seminar Spring 2006. A Comparison of Bacterial Reduction between Antiseptics

CHPPM-West, Fort Lewis.Jose Avila

Page 24: Biology 402 Senior Seminar Spring 2006. A Comparison of Bacterial Reduction between Antiseptics

Comparison of bacterial growth in the oral cavity between

tobacco consumers and non–tobacco usersKrystle Antolin

Page 25: Biology 402 Senior Seminar Spring 2006. A Comparison of Bacterial Reduction between Antiseptics

Range of Motion

• Range of Motion (ROM) is the complete movement of the joint through flexion and extension.

• Normal ROM: extension, 0 degrees and flexion is 135 degrees.

Courtney Rogers

Page 26: Biology 402 Senior Seminar Spring 2006. A Comparison of Bacterial Reduction between Antiseptics

Materials• Active Ankles

brace:

http://www.wisdomking.com/product55238.html

http://www.activeankle.com/

• Lace-up Ankle brace:

Goniometer:Goniometer: http://www.orthobionics.com/ankle/pages/laceup_ankle.htm

Randi-Lee Rezents

Page 27: Biology 402 Senior Seminar Spring 2006. A Comparison of Bacterial Reduction between Antiseptics

Foot Care : A Study Examining The Occurrence of Corns & Calluses in

Males and Females

By Ann Marie McCanickSenior Seminar

May 3, 2006Dr. Hartman & Dr.

Olney