"_thods in Enzymology

* Volume 157

BiomembranesPart Q

ATP-Driven Pumps and Related Transport :Calcium, Proton, and Potassium Pump s

Sidney FleischerBecca Fleischer

CONTRIBUTORS TO VOLUME 157 X i

PREFACE XVii

VOLUMES IN SERIES Xi X

Section I . Cal+ Fluxes and Regulation

1. Membrane Transport of Calcium : An Overview ERNESTO CARAFOLI

3

A. Complex Systems and Subcellular Fractions Involve din Cat+ Regulation

2 . Measurement of Cat+ Release in Skinned Fibers M . ENDO AND M . IINO

1 2from Skeletal Muscl e

3 . Isolation and Characterization of Sarcolemmal STEVEN SEILER AN DVesicles from Rabbit Fast Skeletal Muscle

SIDNEY FLEISCHER

26

4 . Isolation of Sarcoplasmic Reticulum Fractions ALICE CHU, MARK C . DIXON ,Referable to Longitudinal Tubules and Junc- AKITSUGU SAITO ,tional Terminal Cisternae from Rabbit Skeletal STEVEN SEILER, AN DMuscle

SIDNEY FLEISCHER

3 6

5 . Isolation of the Junctional Face Membrane of Sar- BRIAN COSTELLO ,coplasmic Reticulum

CHRISTOPHER CHADWICK ,

AND SIDNEY FLEISCHER

46

6 . Isolation of Triads from Skeletal Muscle

R . D . MITCHELL ,PHILIP PALADE ,

AKITSUGU SAITO ,

AND SIDNEY FLEISCHER

5 1

7 . Isolation of Transverse Tubule Membranes from ANTHONY H . CASWELL ,Skeletal Muscle : Ion Transport Activity, Refor- NEIL R . BRANDT ,mation of Triad Junctions, and Isolation of J-P . BRUNSCHWIG, ANDJunctional Spanning Protein of Triads

RICHARD M . KAWAMOTO

68

8 . Rapid Preparation of Canine Cardiac Sarcolemmal LARRY R . JONES

85

Vesicles by Sucrose Flotatio n

9 . Isolation of Canine Cardiac Sarcoplasmic Retic- BRIAN K . CHAMBERLAI Nulum

AND SIDNEY FLEISCHER

91

10. Junctional and Longitudinal Sarcoplasmic Reticu- MAKOTO INUI ,

turn of Heart Muscle

SHERRY WANG ,

AKITSUGU SAITO ,

AND SIDNEY FLEISCHER

10 0

11. Regulation of Cat+ -Pump from Cardiac Sarcoplas- MICHIHIKO TADA ,mic Reticulum

MASAAKI KADOMA ,

MAKOTO INUI, AND

JUN-ICHI FuJII

107

B. Characterization of Cat+-Pumps and Modulatorsfrom Various Sources

12 . Kinetic and Equilibrium Characterization of an GIUSEPPE INESI ,Energy-Transducing Enzyme and Its Partial Re- MARK KURZMACK ,

actions

AND DAVID LEWIS

154

13 . Approaches to Studying the Mechanisms of ATP LEOPOLDO DE MEIs

190

Synthesis in Sarcoplasmic Reticulu m

14 . Fluorimetric Detection and Significance of Con- Y . DUPONT ,formational Changes in Caz+ -ATPase

F . GUILLAIN, AN D

J . J . LACAPERE

206

15 . Synthesis of ATP from Caz+ Gradient by Sarco- E . FASSOLD AN Dplasmic Reticulum Caz+ Transport ATPase

WILHELM HASSELBACH

220

16. Occluded Ca z+

HARUHIKO TAKISAWA

AND MADOKA MAKINOSE

228

17 . Modified Membrane Filtration Methods for Li- MoTONORI YAMAGUCHI

gand Binding on ATP-Driven Pumps during AND TAKAHIDE WATANABE 23 3

ATP Hydrolysi s

18 . Active Cation Pumping of Na+ , K + -ATPase and TIAN Yow TSONG

240

Sarcoplasmic Reticulum Caz+ -ATPase Induce d

by an Electric Fiel d

19 . Chemical Derivatization of Ca z+ -Pump Protein MASAO KAWAKITA ,from Skeletal Muscle with N-Substituted KIMIKO YASUOKA-YABE ,

Maleimides and 5-(2-Todoacetamidoethyl)ami- KIMIKO SAITO-NAKATSUKA ,

nonaphthalene 1-Sulfonate

AKIKO BABA, AN D

TETSURO YAMASHITA

25 1

20 . Use of Detergents to Solubilize the Caz+ -Pump JESPER V . MOLLER ,Protein as Monomers and Defined Oligomers MARC LE MAIRE, AN D

JENS P. ANDERSEN

26 1

21 . Analysis of Two-Dimensional Crystals of Caz+- K. A . TAYLOR, L . Dux ,ATPase in Sarcoplasmic Reticulum

S . VARGA, H . P . TING-BEALL ,AND A. MARTONOSI

27 1

22 . cDNA Cloning of Sarcoplasmic Reticulum Pro- CHRISTOPHER J . BRANDL ,terns

LARRY FLIEGEL, ANDDAVID H . MACLENNAN

289

23 . Reconstitution of Skeletal Muscle Sarcoplasmic LIN HYMEL AN D

Reticulum Membranes : Strategies for Varying SIDNEY FLEISCHER

302the Lipid/Protein Rati o

24 . Reconstitution of Calcium Pumping of Cardiac MAKOTO INUI AN DSarcoplasmic Reticulum

SIDNEY FLEISCHER

31 4

25. Purification and Crystallization of Calcium-Bind- ANDREAS MAURER ,

ing Protein from Skeletal Muscle Sarcoplasmic MASASHI TANAKA ,

Reticulum

TAKAYUKI OZAWA, AN D

SIDNEY FLEISCHER

32 1

26 . Purification of Calmodulin and Preparation of Im- THOMAS J . LUKAS AN D

mobilized Calmodulin

D . MARTIN WATTERSON

328

27 . Purification, Reconstitution, and Regulation of JOHN T . PENNISTON ,Plasma Membrane Cat+ -Pumps

ADELAIDA G . FILOTEO ,

CAROL S . MCDONOUGH ,

AND ERNESTO CARAFOLI

340

28 . Isolation and Reconstitution of Caz+ -Pump from WILLIAM L . DEAN

35 1

Human and Porcine Platelet s

29 . Purification of Phospholamban from Canine Car- LARRY R . JONES ,

diac Sarcoplasmic Reticulum Vesicles by Use ADAM D . WEGENER, AN D

of Sulfhydryl Group Affinity Chromatography HEATHER K . B . SIMMERMAN 360

30 . Modification of Phospholipid Environment in Sar- JOEL LUNARDI ,coplasmic Reticulum Using Nonspecific Phos- PAUL DEFOOR, AN D

pholipid Transfer Protein

SIDNEY FLEISCHER

36 9

C . Caz+ and Other Channel s

31 . Computer Programs for Calculating Total from ALEXANDRE FABIATO

37 8

Specified Free or Free from Specified Tota l

Ionic Concentrations in Aqueous Solution s

Containing Multiple Metals and Ligand s

32 . Ionic Permeability of Isolated Muscle Sarcoplas- GERHARD MEISSNER

41 7mic Reticulum and Liver Endoplasmic Reticu-

lum Vesicle s

33. Permeability of Sarcoplasmic Reticulum

MICHIKI KASAI AN D

KAZUKI NUNOGAKI

43 7

34 . Measurement of Calcium Release in Isolated NORIAKI IKEMOTO ,

Membrane Systems: Coupling between the Do HAN KIM, AND

Transverse Tubule and Sarcoplasmic Reticulum BOZENA ANTONIU

46 9

35 . Techniques for Observing Calcium Channels from JEFFREY S . SMITH ,

Skeletal Muscle Sarcoplasmic Reticulum in Pla- ROBERTO CORONADO ,

nar Lipid Bilayers

AND GERHARD MEISSNER

48 0

36 . Purification of Caz+ Release Channel (Ryanodine MAKOTO INUI AN D

Receptor) from Heart and Skeletal Muscle Sar- SIDNEY FLEISCHER

49 0

coplasmic Reticulum

37 . Measurement of Sodium-Calcium Exchange Ac- JOHN P . REEVES

50 5tivity in Plasma Membrane Vesicle s

Section II . ATP-Driven Proton Pumps

38 . Plasma Membrane ATPase from the Yeast Schizo- JEAN-PIERRE DUFOUR ,

saccharomyces pombe

ANTOINE AMORY, AN D

ANDRE GOFFEAU

51 3

39 . Plasma Membrane ATPase from the Yeast Sac- ANDRE GOFFEAU AND

charomyces cerevisiae

JEAN-PIERRE DUFOUR

52 8

40 . H + -ATPase from Plasma Membranes of Sac- RAM6N SERRANO

53 3charomyces cerevisiae and Avena sativa Roots :Purification and Reconstitution

41. Purification of Yeast Vacuolar Membrane H + - ETSUKO UCHIDA ,ATPase and Enzymological Discrimination of YosHINORI OHSUMI ,Three ATP-Driven Proton Pumps in Saccharo- AND YASUHIRO ANRAKU

544myces cerevisiae

42 . Purification of Vacuolar Membranes, Mitochon- EMMA JEAN BOWMANdria, and Plasma Membranes from Neurospora AND BARRY J. BOWMAN

562crassa and Modes of Discriminating among th eDifferent H + -ATPase s

43 . Large-Scale Purification ofPlasma Membrane H + - GENE A . SCARBOROUGH

574ATPase from a Cell Wall-Less Mutant of Neu-rospora crassa

44 . H + -ATPase from Vacuolar Membranes of Higher ALAN B . BENNETT ,Plants

ROGER A . LEIGH, AN D

ROGER M . SPANSWICK

579

45 . Preparation and Acidification Activity of Lyso- DONALD L . SCHNEIDER

somes and Lysosomal Membranes

AND JEAN CHIN

59 1

46 . Analysis of Endosome and Lysosome Acidifica- CYNTHIA J . GALLOWAY ,tion in Vitro

GARY E . DEAN ,

RENATA FUCHS ,

AND IRA MELLMAN

60 1

47 . Proton ATPases in Golgi Vesicles and Endoplas- ANSON LOWE ANDmic Reticulum : Characterization and Reconsti- QAIS AL-AWQATI

61 1tution of Proton Pumping

48 . Chromaffin Granule Proton Pump

NATHAN NELSON ,

SHULAMIT CIDON, AND

YOSHINORI MORIYAMA

61 9

49 . Proton Pump of Clathrin-Coated Vesicles

XIAO-SONG XIE ,

DENNIS K . STONE ,AND EFRAIM RACKER

634

Section III . ATP-Driven K + Pumps

50. Preparation of Gastric H + ,K +-ATPase

EDD C . RABON, WHA BIN IM ,

AND GEORGE SACHS

649

51 . Genetics of Kdp, the K + -Transport ATPase of JAMES W . POLAREK ,Escherichia coli

MARK O. WALDERHAUG ,

AND WOLFGANG EPSTEIN

655

52 . K + -ATPase from Escherichia coli : Isolation and ANNETTE SIEBERS ,Characterization

LESZEK WIECZOREK, AN D

KARLHEINZ ALTENDORF

668

53. Purification of the ATPase of Streptococcus MARC SOLIO zfaecalis

AND PETER FÜRST

680

Addendum

Addendum to Article [30]

JOEL LUNARDI ,

PAUL DEFOOR, ANDSIDNEY FLEISCHER

693

AUTHOR INDEX 69 5

SUBJECT INDEX 713

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