bioo scientific - improving the performance of sureselectxt2 target capture
TRANSCRIPT
A solution-based hybridization target capture system that enriches
for regions of interest, as opposed to whole-genome sequencing
A wide range of predesigned probesets or baits and flexible customization
options allows users to design theirown capture panels
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DNA ISOLATION
LIBRARY PREP WITH BARCODES
HYBRIDIZE WITH BLOCKERS
HYBRIDIZE SURESELECT LIBRARIES
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DNA ISOLATION
LIBRARY PREP WITH BARCODES
HYBRIDIZE WITH BLOCKERS
HYBRIDIZE SURESELECT LIBRARIES
WASH & RECOVER CAPTURED DNA
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DNA ISOLATION
LIBRARY PREP WITH BARCODES
HYBRIDIZE WITH BLOCKERS
HYBRIDIZE SURESELECT LIBRARIES
AMPLIFY CAPTURED DNA
WASH & RECOVER CAPTURED DNA
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DNA ISOLATION
LIBRARY PREP WITH BARCODES
HYBRIDIZE WITH BLOCKERS
HYBRIDIZE SURESELECT LIBRARIES
AMPLIFY CAPTURED DNA
WASH & RECOVER CAPTURED DNA
QUANTITATE USING qPCR
Co
ntain
ed
in B
ioo
Scie
ntifi
c’s Kit
Now that we’ve seen the protocol, let’s focus on Step 3: HYBRIDIZE WITH BLOCKERS
0303 HYBRIDIZE WITH BLOCKERS
Index-specific barcode blockers are used & pooled depending upon the indexes used
during library prep
Index-specific barcode blocking strategies improves blocking of adapter sequence
during hybridization
Index-specific barcode blocking strategies result in higher % of on-target reads
NEXTflex Agilent
Targeted Bases 10,180,000 10,180,000Reads Mapped 57,608,301 55,621,420
% Unique / Duplication 95.5 / 4.5 95.7 / 4.3% On Target 83.3 78.5
Mean Coverage 151.2 137.8
Index-specific barcode blocking strategies result in better coverage statistics
NEXTflex Agilent
Targeted Bases 10,180,000 10,180,000Reads Mapped 57,608,301 55,621,420
% Unique / Duplication 95.5 / 4.5 95.7 / 4.3% On Target 83.3 78.5
Mean Coverage 151.2 137.8
The NEXTflex™ Pre- & Post- Capture Combo Kit (Agilent SureSelectXT2 Compatible) incorporates
index-specific barcode blocking strategies
The NEXTflex™ Pre- & Post- Capture Combo Kit (Agilent SureSelectXT2 Compatible) includes:
NEXTflex™ Rapid DNA-Seq library prep reagentsIndexed barcodes
Index-specific blockersHybridization and wash buffers
Post-capture amplification reagents
Learn more about the NEXTflex™ Pre- & Post- Capture Combo Kit (Agilent
SureSelectXT2 Compatible)
PURCHASE KIT DOWNLOADWHITEPAPER
If you have any questions email us [email protected]
or visit our website atBiooScientific.com/SureSelect