biopac slides 090921

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    BIOPAC Systems, Inc.

    Training Seminar

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    About BIOPAC

    Hardware Setup

    Software Setup

    Subject Preparation Data Acquisition Demonstration

    Data Analysis

    Application Notes and Other Resources Question and Answer Session

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    MP150

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    MP150 Range of the MP150 system: from -10 to +10 Volts

    A/D resolution: 16 bit

    Dual A/D converters: Reduce Channel/Channeltiming latency

    65536 levels or 30 microvolts resolution

    Minimum change that can be detected is 30 uvolts. If

    the signal is not strong, it must be amplified.

    A/D Conversion

    Sample Rate

    Max Sample Rate = 200 K Samples/Sec. Maxaggregate of 400 kHz

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    Sampling RateECG waveform sampled with relatively fewsamples per second. The black dots arethe sample points.

    Above waveform as it would look if plotted.

    Representation of the same ECGwaveform sampled at a relatively highersampling rate.

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    Recommended sample rates for physiological signalsSampling RateECG 200 Hz (1,000 Hz for HRV)

    Respiration 50 Hz

    GSR 50 Hz

    EMG 1,000 Hz to 10,000 Hz

    EEG 200 Hz

    EOG 200 HzBlood pressure 100 Hz

    Flow rate 50 Hz

    EGG (electrogastrogram) 1 Hz

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    UIM100C Universal Interface 16 analog channels

    Input range between

    100 mv and 10 v

    16 digital outputs

    Digital Trigger

    Channels are input andoutput

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    Amplifier Settings Red Channel Switch

    Only one amplifier per channel

    Gain

    Depends on application

    Filters AC for Biopotential Recording

    DC for Transducer Recording

    Transducer Amplifiers: Calibration Required

    Biopotential Amplifiers: No Calibration Required

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    ECG100C Amplifier Designed to pass the ECG signal (P, Q, R, S,

    T waves) with minimal distortion

    Works with disposable or reusable electrodes

    Typical Human Settings:

    GAIN: 2000

    MODE: NORM35Hz LP: ON

    HP: 0.5 Hz

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    Filters: Low pass, High Pass, Band Pass, Band Stop, Notch

    Low Pass at 5Hz

    High Pass at 5Hz

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    Filters: A practical Low Pass Filter

    example with ECG Part I

    Noisy ECG data

    Data after applying a low pass filter at 35Hz

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    Filters: A practical high pass filter

    example with ECG Part II

    Drifting ECG data

    Data after applying a high pass filter at 1Hz

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    DC vs. AC example with skin conductance

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    Software SetupBeforeyou start

    recording:

    MP150 Menu

    Set Up Acquisition

    Manual recording

    Automatic recording

    Sample rates

    Acquisition length

    Online Averaging

    Evoked Response

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    Software SetupBeforeyou start

    recording:

    MP150 Menu Set Up Channels

    Analog, Digital, andCalculation Channels

    Scaling andCalibration

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    CALIBRATION GSR Scaling

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    Software Checklist Set analog, digital, and calculation channels

    Make sure analog channels match amplifierchannels

    Make sure Source channels are set in calculationchannel setup

    Set Acquisition Type and Length

    Set up triggering or stimulator if desired

    Make sure green light is on

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    Electrodes, Leads, and Gels

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    Subject Preparation Abrade the skin (ELPADs)

    Electrode Gel

    Wait 5 minutes for best results

    Minimal movement

    Impedance check (EEG, etc.)

    One ground per subject!

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    Einthovens Triangle

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    Hints for minimizing data error Subject should remain as relaxed as possible

    Subject must avoid excessive extraneous movement

    Press ESC to add a marker every time you give the subjectan instruction

    Add text to describe all interventions

    During comparative data analysis, ensure that all scales arethe same for every channel

    Remove all jewelry or other metal objects

    Check all cable connections

    RECORD BASELINE PERIOD!

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    Hardware ChecklistBeforeyou start recording:

    Make sure there is only one amp per channel

    Check the GAIN and filter settings

    Check Subject

    Check electrode placement

    Let gel soak in (5 minutes)Make sure there is only one ground per subject

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    Software Setup Set Up Triggering

    Set Up Stimulator

    Set Up EventHotkey

    Show Input Values

    Show ManualControl

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    Data Analysis Transform and Analysis Menus

    All post-acquisition processes Offline filtering Waveform math

    Fast-Fourier Transform Automated analysis

    Find Rate functions Find Cycle/Peak functions Specialized Analysis Package

    AcqKnowledge4 Windows AcqKnowledge3.9 Mac

    Always duplicate waveform!!

    Specialized Analysis package included

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    Controlling the Display Vertical Scaling

    Autoscaling

    Horizontal Scaling

    Scrolling vs. Autoplot

    Zoom Tool

    Display Modes

    Chart mode, Scope mode,

    X/Y plot, Stacked Plot

    Hiding and DisplayingChannels

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    Filters: Role of coefficients in FIR filters

    Original data

    FIR 35Hz LP coefficients = 4

    FIR 35Hz LP coefficients = 22

    Frequency response for FIR 35Hz LPwith 4 coefficients:

    Frequency response for FIR 35Hz LPwith 22 coefficients:

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    Filters: Performance of FIR filters vs. IIR filters

    Original data

    FIR 35Hz LP coefficients = 22

    IIR 35Hz LP

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    Online Resources Application notes

    Knowledge base

    Product Resources tab

    Publications

    http://www.biopac.com/ApplicationNotes.asphttp://www.biopac.com/ApplicationNotes.asphttp://www.biopac.com/ApplicationNotes.asphttp://www.biopac.com/FAQs.asp