BIOPSY TECHNIQUES FOR BIOPSY TECHNIQUES FOR CHILDREN WITH CANCERCHILDREN WITH CANCER

B.A.JABOB.A.JABO

OUT LINEOUT LINE

• INTRODUCTION• PREPARATION• TECHNIQUES• TYPES• CONCLUSIONS

IntroductionIntroduction

• In the past, malignant conditions in children were primarily diagnosed by histopathologic techniques performed on specimens obtained during surgery and involved extirpation of a mass or open biopsy.

contcont

• Improvements in diagnostic imaging, newer techniques in molecular biology, and progress n surgical techniques have permitted accurate diagnosis and staging of many types of childhood tumors using minimally invasive approaches.

• Simple hybridization strategies & southern blotting were originally used.

contcont

• Fluorescence insitu hybridization (FISH), polymerase chain reaction (PCR) and reverse transcriptase.

PreparationPreparation

• Consent• Haematological investigations• Biochemical• Bowel

TechniquesTechniques

• Open• Percutaneous• Endoscopy• Laparoscopy• Thoracoscopy

TypesTypes

• Incisional• Excisional• Percutaneous (FNAC, TRUCUT)• Frozen sections

ConclusionConclusion

• Important – diagnosis & prognosis• Management plan• Multi displinary team work• Long term follow up

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