bioremediation (1) - ufz · bioremediation: a bugs life! microorganisms….but don’t forget the...
TRANSCRIPT
![Page 1: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/1.jpg)
Bioremediation (1)
1
![Page 2: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/2.jpg)
Bioremediation (1):
Microbiological Basics
Literatur:
F.H. Chapelle
Groundwater-Microbiology and Geochemistry
Central Questions:
- Why works in situ-BIOREMEDIATION always?
- Which role play organic contaminants?
2
![Page 3: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/3.jpg)
Bioremediation: A Bugs Life!
….but don’t forget the plants Microorganisms (Bacteria) are the „Key players“ in
BIOREMEDIATION: ubiquitary occurence!
![Page 4: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/4.jpg)
Innovative Reactive Barrier Technologies for Regionally Contaminated Aquifers
- Sessile Bacteria colonies
- near main flow pathes
- wander to ED + EA-gradients
4
![Page 5: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/5.jpg)
Cfu (g-1
sediment)
100 101 102 103 104 105 106
Dep
th [
m]
4
6
8
10
12
14
16
18
20
22
24
26
28
30
32
34
36
38
40
42
44
46
48
50
Aerobic bacteria (R2A agar)
Anaerobic bacteria (TSI agar)
Quarternary aquifer
Lignite seam
Tertiary aquifer
Bacterial colonization of the subsurface at the test site (SAFBIT 1/97 and 2/97)
even though oxygen(EA) is not present,
the aerob degradation pathway can be used!
important for oxygen-Injection 5
![Page 6: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/6.jpg)
Perchlorate - Degrading Bacteria
6
![Page 7: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/7.jpg)
Bioremediation: A Bugs Life!
….but don’t forget the plants Energy- and metabolism processes of microorganisms:
What is the energy source?
Redox-Processes Energy sources!
7
![Page 8: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/8.jpg)
e-
Cl- + O2
Biomass + CO2 Substrate (C-Quelle)
ClO4-
NO3-
O2
Example: Biological Perchlorate Degradation
Electron donors
e.g. all organic
contaminants (BTEX)
Electron acceptors
Energy gain through electron transfer
from Electron donor to Electron acceptor
ED: Plus-Pol
EA: Minus-Pol
8
![Page 9: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/9.jpg)
Bioremediation:
Processes and Tools
- Basic course 1: Redox-Chemistry
9
![Page 10: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/10.jpg)
- Basic course 1: Redox-Chemistry
1. Redox processes?
2. Oxidation and Reduction ?
3. Determine electron donor and acceptor with the help
of the Partial charge concept (PCC)!
Redox-Processes energy sources
10
![Page 11: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/11.jpg)
11
![Page 12: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/12.jpg)
O2
H2O
Den
itrif
icati
on
Perc
hlo
rate
Red
uct
ion
ClO4 NO3 CO2 SO4
N2 H2S CH4 Cl-
+ 800 - 250
Redox (millivolts)
Electron
Donor
Energy gain through electron transfer
from electron donor to electron acceptor
0
Klausur: Apply the PCC for all Redox-Pairs: EA?, ED?
C6H6
12
![Page 13: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/13.jpg)
„Current“ = electron transport from electron donor (ED) to
electron acceptor (EA) like a battery
Redox-Potential-Difference determines the „Current“, that
means how fast the organic contaminant (ED) will be degradaded
necessary for microbial degradation: Minus-Pole (EA)
Limiting Factor: Absence of EA‘s (e.g. Oxygen)
13
![Page 14: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/14.jpg)
Redox potential decreases!
? mV ? mV
? mV
Which Redox-Potential (voltage) measure
the Redox-electrodes? 14
Redox-Zonation in a contaminated aquifer
![Page 15: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/15.jpg)
O2
H2O
Den
itrif
icati
on
Perc
hlo
rate
Red
uct
ion
ClO4 NO3 CO2 SO4
N2 H2S CH4 Cl-
+ 800 - 250
Redox (millivolts)
Electron
Donor
Energy gain through electron transfer
from electron donor to electron acceptor
0
Klausur: Apply the PCC for all Redox-Pairs: EA?, ED?
C6H6
15
![Page 16: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/16.jpg)
Redox-Zonation in a pure, uncontaminated aquifer
Recharge
Redox potential decreases!
? mV
? mV
? mV ? mV
? mV
16
Task: Determine the potential!
![Page 17: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/17.jpg)
Energy gain through reductive Dechlorination:
PCE + H2 TCE + Cl- + H+
EA, ED ?
17
![Page 18: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/18.jpg)
18
![Page 19: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/19.jpg)
19
![Page 20: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/20.jpg)
20
𝑓𝐸𝐴,𝐸𝐷 ≡ 𝑈𝐹(𝐸𝐴, 𝐸𝐷) =𝑚𝐸𝐴
𝑚𝐸𝐷
HÜ8 (ÜA16, ÜA25)
BIOREM-Protokoll: Quantifizierung von Abbauprozessen
1. Feldsituation/Standort
2. Monitoring/Hypothesen
- Charakterisierung der Schadstoffquelle, Schadstofffahne
- Schadstoff, mögliche Abbauwege
3. Bilanzierung/Quantifizierung
3.1. Abbaupotenzial (BC) des GWL
- zentrale Frage: BC groß genug um Schadstoff abzubauen?
3.2. Abbau-Kinetik: Steady-State AD-Glg
3.3. Inverse Modellierung: - Bioremediation Ja/Nein?
- Schätzung der Abbaurate (kbio)
- Schätzung der Schadstoffmasse (C0)
3.4. BIOSCREEN alles in 2D!
![Page 21: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/21.jpg)
21
BioREM-Protokoll: 3.2. Abbaukinetik für Toluen und Benzen
Toluen
Benzen
Rote Kurve:
Kurven-Fit an Felddaten: Inverse Modelling
LAbbau
![Page 22: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/22.jpg)
Aerobic and anaerobic
degradation pathways
EA = oxygen aerobic degradation pathway (max. energy gain)
EA = Nitrat et al. anaerobic degradation pathway
22
![Page 23: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/23.jpg)
Bioremediation: A Bugs Life!
….but don’t forget the plants Aerobe und anaerobe Abbauwege für
typische organische Schadstoffe 23
![Page 24: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/24.jpg)
Strukturformeln
wichtiger org. Schadstoffe
= Elektronendonatoren
Skript
24
PCE, TCE,
DCE, VC
BTEX
MCB, DCB
MTBE
![Page 25: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/25.jpg)
Schadstoffhäufigkeit im Abstrom von Schadensfällen
60 50 40 30 20 10 0 0 10 20 30 40 50 60
TCE
PCE
Dichlorethen (trans)
Trichlormethan
Dichlorethen (1,1)
Dichlormethan
Trichlorethan (1,1,1)
Dichlorethan (1,1)
Dichlorethan (1,2)
Phenol
Aceton
Toluol
Diethylhexylphthalat
Benzol
VinylchloridDeutschland USA
TCE
PCE
Dichlorethen (cis)
Benzol
Vinylchlorid
Trichlormethan
Trichlorethan (1,1,1)
Xylol
Dichlorethen (trans)
Toluol
Ethylbenzol
Dichlormethan
Dichlorbenzol
Chlorbenzol
Tetrachlormethan
Schadensfälle / % nach G. Teutsch, P. Grathwohl, 1997
25
![Page 26: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/26.jpg)
Grundwasserkontamination - Beispiel Bitterfeld
Jessnitz
Wolfen
Muldenstein
RossdorfBurgkemnitz
Schlaitz
Altjessnitz
Raguhn
KledewitzThurland
Reuben
Thalheim
Sandersdorf
B100
B184
> 1000 µg/L AOX
300 - 1000 µg/L AOX
60-300 µg/L AOX
20 - 60 µg/L AOX
10 - 20 µg/L AOX
Bitterfeld
Biedersdorf
Mühlbeck
Pouch
A 9
5 km
Dichloromethan
1,1-Dichlorethen
cis-Dichlorethen
trans-Dichlorethen
Trichlormethan
1,1-Dichlorethan
1,2-Dichlorethan
1,1,1-Trichlorethan
1,1,2-Trichlorethan
Tetrachlormethan
Trichlorethen
Tetrachlorethen
1,1,1,2-Tetrachlorethan
1,1,2,2-Tetrachlorethan
Pentachlorethan
Hexachlorethan
Vinylchlorid
Benzen
Toluen
Chlorbenzen
1,2-Dichlorbenzen
1,3-Dichlorbenzen
1,4-Dichlorbenzen
25 km2 mit einem geschätzten Volumen von mehr als
200 Mio m3 kontaminiertem Grundwasser
1,2,3-Trichlorbenzen
1,2,4-Trichlorbenzen
1,2,5-Trichlorbenzen
1,3,5-Trichlorbenzen
2-Chlorphenol
3-Chlorphenol
4-Chlorphenol
2,3-Dichlorphenol
2,4-Dichlorphenol
2,5-Dichlorphenol
2,6-Dichlorphenol
3,4-Dichlorphenol
3,5-Dichlorphenol
2,3,4-Trichlorphenol
2,3,5-Trichlorphenol
2,3,6-Trichlorphenol
2,4,6-Trichlorphenol
2,3,4,5-Tetrachlorphenol
2,4,5,6-Tetrachlorphenol
Pentachlorphenol
4-Chlor-3-methylphenol
1-Chlor-3-methylphenol Sanierungsforschung
in regional kontaminierten
Aquiferen
26
![Page 27: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/27.jpg)
27
Destabilisierendes
Dipolmoment!
Abbauweg erfolgt über Catechol!
![Page 28: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/28.jpg)
Aerober Abbauweg von Benzen zu Catechol Ringspaltung
28
![Page 29: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/29.jpg)
Aerober Abbauweg von Chlorbenzen
29
![Page 30: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/30.jpg)
Möglicher aerober Abbauweg
von MTBE
30
![Page 31: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/31.jpg)
Abbauwege höher-
chlorierter KWs
31
HÜ8 – Aufgabe 25: Gekoppelter PCE-TCE-Abbau
Zur Wiederholung
Start SS2015: 17.4.2015
![Page 32: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/32.jpg)
Bioremediation: A Bugs Life!
….but don’t forget the plants Höher-chlorierte Kohlenwasserstoffe
sind häufig persistent gegenüber aeroben Abbau
HCH, PCE
Case study: Auensee-Leipzig
32
![Page 33: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/33.jpg)
TCE PCE TCA
Case study: Auensee-Leipzig
Exkursion!
lignite seam
Auensee
H2-O2-Injektion
33
![Page 34: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/34.jpg)
Case study: Auensee-Leipzig
34
![Page 35: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/35.jpg)
Case study: Auensee-Leipzig
35
![Page 36: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/36.jpg)
HÜ8: ÜA 16 + 25: Bioremediation
Dechlorierungsreaktion: PCE + 2e- + H+ TCE + Cl- : (kPCE)
TCE + 2e- + H+ DCE + Cl- : (TCE)
Differentialgleichungen für „Parents-Daughter-Reaction“:
PCE
PCE
PCEPCE
PCE
PCE CR
k
x
C
R
u
t
C
PCE
TCE
PCETCE
TCE
TCETCE
TCE
TCE CR
kfC
Rx
C
R
u
t
C
Reine Advektion:
![Page 37: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/37.jpg)
Steady-state-Lösung:
C(x,t) – Konzentration (mg/l), k – Abbaurate (1/Tag),
u – Abstandsgeschwindigkeit (m/Tag)
Diskussion der ÜA25 Skript: ÜA mit Lsgn
EXCEL-file: steady-state (siehe ÜA25 Bioremediation!)
x
u
kxCxC PCE
PCEPCE exp)0()(
x
ux
u
k
k
xCkfxC TCEPCE
PCETCE
PCEPCETCE
expexp
)0()(
Keine Zeitabhängigkeit!
x
u
kxCtkfxC PCE
PCEPCETCE exp)0()(
PCETCE k
PCETCE k
![Page 38: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/38.jpg)
Tools zur
Bioremediation-
Technologie-Optimierung
Biocapacity-Konzept:
Welches Abbaupotential besitzt unser Aquifer?
Start SS2015: 17.4.2015
![Page 39: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/39.jpg)
39
![Page 40: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/40.jpg)
40
HÜ9
![Page 41: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/41.jpg)
41
Field data
![Page 42: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/42.jpg)
42
Field data
![Page 43: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/43.jpg)
43
Field data
![Page 44: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/44.jpg)
Bioremediation:
BIOSCREEN-Course
Reaktiver Transport BIOSCREEN
![Page 45: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/45.jpg)
45
Transport von Schadstoffen:
Mathematische Beschreibung (3)
Ddisp = Dispersionskoeffizeint
siehe Skript!
4. advektiver, dispersiver Transport („diffusiver“ Transport im strömenden Grundwasser) :
dx
dCu
dx
CdD
dx
Cujd
dt
dC TT
Tdisp
TTTT
2
2)(TldispdispdispwT uDRDDRuu ,/,/ 0
Lösung für konstante Randbedingung (ÜA 22):
),(),(
2),( 0 txgErfc
D
xuExptxgErfc
CtxC
disp
TT
tD
tuxtxg
disp
T
2),(
0),0( CtxCT
Task 22-3, Task 23-2
![Page 46: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/46.jpg)
46
Transport von Schadstoffen:
Mathematische Beschreibung (4)
Reaktiver Transport
RT
TT
dispT Q
dx
dCu
dx
CdD
dt
dC
2
2
TldispdispdispwT uDRDDRuu ,/,/ 0
2. Steady-state-Annahme:
0)0( CxCT
TR CkQ 1. Für Reaktionsrate verwenden wir Kinetik 1.Ordnung:
TT
TT
dispT kC
dx
dCu
dx
CdD
dt
dC
2
2
TT
TT
disp kCdx
dCu
dx
CdD
2
2
0
3. Berücksichtigen nur konvektiven Transport:
TT
T kCdx
dCu
Analytische Lösung (steady-state, 1. order, reaktiver Transport) :
)/exp()( 0 uxkCxCT
BIOSCREEN: Centerline
HÜ8
![Page 47: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/47.jpg)
1) Solute transport without decay (k = 0!),
2) Solute transport with biodegradation modeled as a
first-order decay process
3) Solute transport with biodegradation modeled as
an "instantaneous" biodegradation reaction
BIOSCREEN
EXCEL: Extras Makros Sicherheit niedrig
Wer Software haben will, bitte e-mail an mich!
![Page 48: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/48.jpg)
2) Solute transport with biodegradation modeled as a
first-order decay process
![Page 49: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/49.jpg)
1 EA = Oxygen
Nur wenn EA vorhanden, kann
BTEX abgebaut werden, d.h.
C0_TCE(NA) <= BC!
3) Solute transport with biodegradation modeled as
an
"instantaneous" biodegradation reaction
![Page 50: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/50.jpg)
![Page 51: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/51.jpg)
![Page 52: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/52.jpg)
![Page 53: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/53.jpg)
![Page 54: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/54.jpg)
![Page 55: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/55.jpg)
Bioremediation-
Seminar 1 + 2
BIOSCREEN for SAFIRA test site LEUNA
Biodegradation of BTEX/MTBE
55
![Page 56: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/56.jpg)
56
![Page 57: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/57.jpg)
57
Field data
![Page 58: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/58.jpg)
58
Field data
![Page 59: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/59.jpg)
59
Field data
![Page 60: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/60.jpg)
60
![Page 61: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/61.jpg)
61
![Page 62: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/62.jpg)
62
![Page 63: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/63.jpg)
63
![Page 64: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/64.jpg)
64
![Page 65: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/65.jpg)
65
![Page 66: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/66.jpg)
66
![Page 67: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/67.jpg)
67
![Page 68: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/68.jpg)
68
![Page 69: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/69.jpg)
69
![Page 70: Bioremediation (1) - UFZ · Bioremediation: A Bugs Life! Microorganisms….but don’t forget the plants (Bacteria) are the „Key players“ in BIOREMEDIATION: ubiquitary occurence!](https://reader034.vdocument.in/reader034/viewer/2022042210/5eaeafe7bdb835361154ee4a/html5/thumbnails/70.jpg)
70