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  • 02-Jul-2015

    BioSigma: Integral

    Biotechnological Solutions for

    the Mining Industry

    Workshop: Chile-Germany Cooperation on Raw

    Materials of Strategic Economic Importance

    September, 2015

    Dr. Roberto Bobadilla-Fazzini

    [email protected]

    BioSigma S.A.

    mailto:[email protected]

  • Contents

    1. BioSigma Research, Development &

    Innovation

    2. BioSigma, Integral Biotechnological

    Applications for the Mining Industry

    3. BioSigma Challenges & Opportunities

  • Confidential - Property of BioSigma

    Introduction to BioSigma

    Founded May 31st, 2002

    Business Area Biotechnologies for mining

    Mission

    To be a company known for

    the development, adaptation

    and implementation of

    integral biotechnological

    solutions based on

    sustainable technologies,

    adding value to mining

    operations and profit to its

    shareholders.

    67% 33%

    http://www.nmm.jx-group.co.jp/english/

  • Confidential - Property of BioSigma

    Introduction to BioSigma:

    IP Rights As an R&D+i company, BioSigma is focused on Technological Intellectual

    Property (IP) generation, protecting its know-how for bioleaching

    applications at commercial-scale.

    20th Inventions

    13 Countries

    90 Granted Patents

  • Confidential - Property of BioSigma

    Wenelen (Iron- & Sulfur-oxidizer)

    Acidithiobacillus ferrooxidans

    Patent Register

    44546

    Yagan (Iron-oxidizer)

    Leptospirillum ferriphilum

    Patent Application

    2077-2007

    Licanantay (Sulfur-oxidizer)

    Acidithiobacillus thiooxidans

    Patent Register

    47819

    BioSigma R&D+i

    Cutipay (Iron- & Sulfur-oxidizer,

    chloride resistant)

    Sulfobacillus thermosulfidooxidans

    Patent Application

    3335-2013

    BioSigma has isolated, characterized and patented different

    microorganisms based on their particular properties to enhance the

    bioleaching process.

  • Confidential - Property of BioSigma

    BioSigma R&D+i

    Cutipay (Iron- & Sulfur-oxidizer,

    chloride resistant)

    Sulfobacillus thermosulfidooxidans

    Patent Application

    3335-2013

    BioSigma has isolated, characterized and patented

    different microorganisms based on their particular

    properties to enhance the bioleaching process,

    including chloride resistant strains.

  • Confidential - Property of BioSigma

    BioSigma R&D+i

    7

    1. Advanced microbial

    characterization analysis

    (patented qPCR, Massive

    Sequencing).

    2. Advanced mineralogical

    analysis (QEMSCAN).

    3. Biomass isolation,

    characterization and

    production (patented

    bioreactors).

    4. Biohydrometallurgical

    assays.

  • Confidential - Property of BioSigma

    BioSigma R&D+i BioSigmas biohydrometallurgical Simulation Platform

    H+ Concentration Fe3+ Concentration

    Position Time Position Time

    Attached Microorganisms Concentration

    Position

    Time

    Inoculation

    8

  • 9

    2002-today LBMG- Universidad de Chile, Chile

    2002-2005 University of Okayama, Japan

    Jan- March 2006 CNRS, France

    Jan 2008 Dec 2010: Institute of Advanced

    Bioscience (IAB) - Keio University, Japan

    Oct-Dec 2009: Universitt Duisburg-Essen,

    Germany

    July-Sept 2011: University of Minnesota, USA

    May 2014: Universidad de Concepcin, Chile

    June 2014-today: TU Freiberg, Germany

    July 2014-today: Helmholtz-Zentrum Dresden -

    Rossendorf

    BioSigma R&D+i: Collaboration

    Confidential Property of BioSigma

    https://www.hzdr.de/

  • Contents

    1. BioSigma Research, Development &

    Innovation

    2. BioSigma, Integral Biotechnological

    Applications for the Mining Industry

    3. BioSigma Challenges & Opportunities

  • Confidential - Property of BioSigma

    BioSigma, Integral Biotechnological

    Applications for the Mining Industry

    Pilot plant

    BioSigma-CELTE pilot plant located 40 km north Santiago is

    equipped with high tonnage columns (12 ton/column). Additionally

    it has solvent extraction electrowinning (SX-EW) equipment to

    perform full biohydrometallurgical assays.

  • Confidential - Property of BioSigma

    BioSigma, Integral Biotechnological

    Applications for the Mining Industry BioSigma CODELCO Radomiro Tomic

    Industrial Biomass Plant:

    Design & Validation of a very efficient air-lift

    bioreactor with a phase separation system

    (Patent Registration No. CL 48319) including: i)

    Fast flow increase; ii) High biomass

    productivity and efficient iron oxidation; iii)

    Constant microbial composition.

    BioSigma CODELCO Radomiro Tomic

    Industrial Bioleaching Plant:

    Industrial validation of BioSigmas

    bioleaching technologies BioSigma-

    SBP and BioSigma-CPY on primary

    sulfide low grade ore.

    12

  • Confidential - Property of BioSigma

    BioSigma, Integral Biotechnological

    Applications for the Mining Industry

    Crushed ore (1 ) Copper recovery after one year operation:

    46.2%

    Conventional: 36.3%

    ROM ore Copper recovery after one year operation:

    34.6%

    Conventional: 23.4%

  • Contents

    1. BioSigma Research, Development &

    Innovation

    2. BioSigma, Integral Biotechnological

    Applications for the Mining Industry

    3. BioSigma Challenges & Opportunities

  • Confidential - Property of BioSigma

    High Optical counting and Cell viability

    Middle to low optical counting and Reduced cell viability

    Ore Heap

    High ionic strength

    solutions

    Bioreactor

    BioSigma Challenges & Opportunities I

    Develop & adapt BioSigmas technologies case-wise, such as in brownfields

    with high ionic strength solutions and/or sea water usage.

    Lab-scale Industrial-scale

  • Confidential - Property of BioSigma

    BioSigma Challenges & Opportunities II

    Explore collaboration on bioremediation:

    ARD Active Treatment

    Sulfate Treatment

    Impact Prediction, Assessment and

    Mitigation

    Biological Active Treatment

    Reliable Mine Waste Management

    Metal Removal

  • 02-Jul-2015

    BioSigma: Integral

    Biotechnological Solutions for

    the Mining Industry

    Workshop: Chile-Germany Cooperation on Raw

    Materials of Strategic Economic Importance

    September, 2015

    Dr. Roberto Bobadilla-Fazzini

    [email protected]

    BioSigma S.A.

    mailto:[email protected]