biosurfactants and bioemulsifiers - phyconet · · 2015-09-24biosurfactants and bioemulsifiers dr...
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Biosurfactants and Bioemulsifiers
Dr Pattanathu Rahman
Biotechnology Programme Leader
Teesside University - UK
&
Founding Director
TeeGene Biotech Ltd.
Wilton Centre, Wilton – UK
http://www.teegene.co.uk
TeeGene Biotech Ltd.TeeGene Biotech Ltd.
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TEESSIDE UNIVERSITY - UK
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Wilton Centre http://www.wiltoncentre.com/
• Reg. No: 09361483
• Registered Office: ONE AMERICA SQUARE, CROSSWALL, LONDON - EC3N 2SG
• Office: H245, WILTON CENTRE, REDCAR – TS104RF
CPINHCNBMCFujifilmFORGETeeGene
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BIOSURFACTANTS
Properties:
� Non-toxic, Non-hazardous
� Thermostable
� Low surface tension
� Emulsify: wide range of hydrocarbons - Stable emulsion
• Teegene biotech is a spin out venture from Teesside University, which is pioneering the use of biosurfactants in a range of cosmetics, environmental and biomedical applications.
• Highly-effective biotechnology based surface-active multi-functional products
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Classification of microbial surfactants
Microbial surfactants
Biosurfactants Bioemulsifier
• Low molecular weight• Composed of sugars,
amino acids and fatty acids
• Reduce surface/interfacial tensions
• Solubilise and emulsify hydrophobic substrates
• High molecular weight• Consists of
polysaccharide-protein lipid complex
• Less effective in surface/interfacial tension reduction
• Efficient emulsification and stabilization of emulsion
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Characteristics• Both bioemulsifiers and biosurfactants contain hydrophobic and
hydrophilic moieties
• The non-polar (hydrophobic) region is often a hydrocarbon chain.
• The polar portion (hydrophilic) region may be ionic (cationic or anionic), non-ionic or amphoteric
(Campos et al., 2013)
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Chemical structures of the conventional mannosylerythritol lipids.
(Fukuoka 2008)
Surfactin (Mulligan, 2009)
(Zhou et al.,2013)
Sophorolipid
Structure of Biosurfactants
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Marine Microbes
Harvey and Rahman, 2015
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Culture conditions required for Algae
Harvey and Rahman, 2015
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Screening of novel microbes from nature
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Biosurfactant Bacteria - Teesside
AM419154 Pseudomonas teessidea partial 16S rRNA gene, type strain PR65T
AM419155 Pseudomonas clemancea partial 16S rRNA gene, type strain PR221T
Culture Collection = 122 bacterial strains from Northeast England
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Scale up of Bioprocess
� 100ml of broth (Nutrient: Glycerol + MSM)
� Conditions: agitation 150rpm, pH 7.0 and incubation at 30oC
Biosurfactant production in batch fermenter (New Brunswick Bioflo 101)
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HPLC / Mass spectrum of rhamnolipids
Mass (m/z)
503.654 g/mol
649.595 g/molS#: 3-62 RT: 0.04-1.00 AV: 60 NL: 2.82E7
F: - c ms [ 200.00 - 800.00]
200 250 300 350 400 450 500 550 600 650 700 750 800
m/z
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
Rela
tive A
bundance
649.5
650.4
503.7
746.9
677.2
762.9648.8
678.3505.0 763.8258.6 502.5
479.3333.3270.7 529.4 621.5 684.6242.7
339.5 703.2601.3573.5451.4367.1 417.4
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Emulsification of hydrocarbons by biosurfactants
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OIL EXTRACTION TECHNIQUE
Tertiary Recovery or Enhanced Oil Recovery (EOR)
•MEOR is a method of tertiary recovery •EOR could potentially recover a further 60 billion barrels (US Report) •Current oil extraction techniques still leave oil trapped in low permeability zones•MEOR can potentially solve this problem. •MEOR is a ‘green’ option to extract remaining reservoir oil
Sandstone
Housing Oil
Shale
Shale
Overburden
Hot Water
Steam Injector
Steam
Oil Reservoir Tertiary Recovery (Collitt, Sina and Rahman 2014)
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BEOR TECHNOLOGY
http://www.huffingtonpost.com/amanda-greene/soy-lecithin-why-is-it-in_b_2891780.html
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BIOSURFACTANT MARKET RESEARCH
�According to F&S estimations the global biosurfactants market rounds $1.76 B (base year 2013), with a combined annual growth rate of about 3.5%
�The global biosurfactant market is expected to reach $2.8 B in 2023
�European markets are leading the biosurfactant space with 53.8% of global sales revenue, followed by North America and then Asia Pacific.
�(Source: Life Sciences and Biotech Technical Insight, Frost & Sullivan, 22 May 2014)
SEKHON, K.K. AND RAHMAN, P.K.S.M. 2014. Frontiers in Microbiology 5:454-460.
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ALGAL EXOPOLYSACCHARIDES
• Algal EPS represent a huge range of structures
• They are high-molecular-weight structures (10-30 kDa)
• EPS structure
• varies widely between different genera of algae
• environmental conditions
(Patel et al., 2010)
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POLYSACHHARIDES - BIOEMULSIFIERS
• Worldwide, production of polysaccharides from marine biomass is between 25,000-30,000 tons/year (Pichavant, 2009)
• polysaccharides can be classified in to three groups: 1. Cytosolic polysaccharides – provide carbon and energy
sources for the cell,
2. Structural polysaccharides - make up the cell wall
3. Exopolysaccharides (EPS) - secreted in to the extracellular environment in capsules or biofilms
(Roger 2002)
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SCREENING OF ALGAE
A. Saltburn, B. Seaton and C. Skinningrove beaches
(Google Maps, 2015).Harvey and Rahman, 2015
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Surface tension measurements Emulsification Index (E24 %) in
Sunflower Oil
Harvey and Rahman, 2015
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Bacterial Bio-emulsifier
Algal bio-emulsifiers
Harvey and Rahman, 2015
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TECHNOLOGY COLLABORATION WITH NETWORK MEMBERS
Green micro-algae Dunaliella salina and red algae Porphyridium cruentum –bioemulsifier producers
ANY SLIMY ALGAE:
Preliminary screening for biosurfactants and bioemulsifiers - Free of cost
Contact me ([email protected])
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ACKNOWLEDGEMENT
� Teesside University
� Commonwealth Scholarship Commission
� Business Investment Funds
� Biobase Europe Pilot Plant (Belgium)
� Research and Development Team
� BSc, MEng, MSc and PhD students
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Thanks
Dr Pattanathu Rahman
Biotechnology Programme Leader
Teesside University - UK
&
Founding Director
TeeGene Biotech Ltd.
Wilton Centre, Wilton – UK
Email: [email protected]