biotecnología aplicada a cultivos de microalgas
DESCRIPTION
En el marco de la jornada Microalgas, ¿una fuente de petróleo verde?, organizada con IMDEA y celebrada el 8 de abril en EOI, Escuela de Organización Industrial, Antonio Fernández del Instituto Biomar, como se usa la biotecnología a cultivos de microalgas. 8_04_2010TRANSCRIPT
BIOTECNOLOGIA APLICADA AL CULTIVO DE MICROALGAS:
BUSQUEDA, SELECCIÓN Y OPTIMIZACION DE MICROALGAS PARA LA PRODUCCION DE ACEITE
COMO BIOCOMBUSTIBLE.BIOTECHNOLOGY APPLIED TO MICROALGAE
CULTURES: SELECTION AND OPTIMIZATION OF MICROALGAE CULTURES FOR BIODIESEL
PRODUCTION.
Instituto Biomar is a 14 year old biotechnological company located in Parque
Tecnológico de León
The company
1996: Spin off from PharmaMar
1997: Move to facilities in biotech business incubator
2000: Start of work in Industrial Applications: Biospesticides
2001: Start of the Fermentation plant
2005: Start of the Microalgae project
2007: Start of the “Microalgae for biodiesel” project.
2009: Opening of new facilties in Leon Technological Park
The company
TECHNOLOGIES APPLICATIONS
The company
Instituto Biomar: fields of interest
MICROBIOLOGY
CHEMISTRY
FERMENTATION
HUMAN HEALTH
FOOD
BIOPESTICIDES
AQUICULTURE
BIODIESEL
The company
The company
The company
BIODIESEL
Metanol + KOH
Trans-esterification Crude
biodiesel Glicerol
Metanol
• Proteins• Sugars• High value
compounds
Photosynthetic
microorganisms
(Biomass)
Acylglicerides
Microorganisms
ActinobacteriaFungi
Biodiesel Project
Biodiesel Project
STRAIN SELECTION
GROWTH RATE OIL CONTENT
OPTIMIZATING PRODUCTION
CLASSIC METHODS GENETIC MANIPULATION
INDUSTRIAL SCALEOPEN SYSTEMS
(RACEWAYS)CLOSED- SYSTEMS(PHOTO-REACTORS)
CO2 Lipids
Chemical transformation
Water
Salts
MICROALGAEBIOMASS
BIODIESEL
EXTRACTION
Sugars, proteins
High value compounds
Biodiesel Project
Isolation from marine invertebrate organisms, water, sediments or soils and plant samples of different sites of the world (Europe, America, Africa…)
Australia
Mediterranean Sea Japan
Ecuatorial Guinea
Canarias
Escocia
HawaiiRed Sea
Spain
Senegal
Argentina
Puerto RicoPanamáGuatemala
MartinicaMéxico
Alaska
NewZeland
NorwayIsland
Chile
Perú
Groenland
Biodiesel Project
Laboratory of Microalgae
ISOLATIONMarine macroorganisms, sediments, rocks, soils…are crushed and cultured on different media (BG-11, ASNIII,…).Isolation process: Picking colonies by hand under stereomicroscopes.
(3000 isolated monocultures per year)
STRAIN SELECTIONDereplication process by sample and sampling point, using stereomicroscope and microscope. This process leads to different strains (aproximately 1200-1400 different monocultures per year).
Laboratory of Microalgae
Current collection of microalgae and cyanobacteria is approximately 2.500 monocultures
STRAIN CULTUREPRIMARY CULTURES on 50 ml flasks are cultured with controlled light and temperature (1200-1400 monocultures per year).
Laboratory of Microalgae
STRAIN CULTURE (2)SECONDARY CULTURES on 200 mL tubes, aireated and with temperature and light controlled (1200-1400 monocultures per year).
Laboratory of Microalgae
STRAIN CONTROLAll strains are photographed, growth is measured, dry weight is estimated, HPLC (UV-vis) profile is determined and DNA is obtained.
With these secondary cultures we obtain biomass for our screening programs:- Drug discovery screening program.- Biodiesel screening program.
Laboratory of Microalgae
SCREENINGAcylglicerides measurementsSamples are extracted with hexane and measured for acylgliceride content (not lipid content) by colorimetry (595 nm) on 96 multiwell plates.
Laboratory of Microalgae
SCREENING Positive samples are re-tested using TLC in order to
diferentially detect acylglicerides (tri-, di-, monoacylglicerides).
TAG
DAGMAG
Laboratory of Microalgae
We select 10-20 strains per year to initiate studies
in order to improve growth and/or productivity.
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% mg AG/mg DW
mg DW · L-1/100
SELECTING STRAINS
Laboratory of Microalgae
IMPROVEMENT- Growth: Testing different conditions on 24 well plates.
- Acylglicerides content: searching for conditions leading to accumulation but with no effects on growth.
Laboratory of Microalgae
SCALE-UPFrom these studies we aim to select 1-2 strains with appropiate characteristics to initiate open air cultures (200-400 L) during 2010, as a step previous to industrial scale up.
Laboratory of Microalgae
1
2 Isolating
3
4
Culturing5
Caracterizing
8
7
6Selecting
Improving
Producing strain
DYNAMIC PROCESS
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% mg TG/mg DW
mg dw/L cult entre 100
Bioprospecting
Profiling
Biodiesel project
Biodiesel project
STRAIN SELECTION
GROWTH RATE OIL CONTENT
OPTIMIZATING PRODUCTION
CLASSIC METHODS GENETIC MANIPULATION
INDUSTRIAL SCALEOPEN SYSTEMS
(RACEWAYS)CLOSED- SYSTEMS(PHOTO-REACTORS)
