Naturally ahead

Biotronic ® Top3Triple action for improved performance!

Per4izer – the breakthrough in animal growth performance

CHALLENGES IN ANIMAL PRODUCTIONCHALLENGES IN ANIMAL PRODUCTION

• high levels of productivity

• high-quality products

• bacterial challenge

Salmonella, E. coli, Campylobacter, Clostridia, Streptococcus…

Feed Water Environment GI tract

kill beneficial microflora

no effect on feed hygiene

drug-resistant bacteria

ANTIBIOTICS VS NATURAL PRODUCTS

reduction of common bacterial burden

improvement of digestibility

Natural products as potential alternative to antibiotics…

ACIDIFICATION

Feed hygiene and GIT health is improved…

Dietary supplementation by acidifiers:

reduces pH and buffer capacity

reduces pathogens in feed and GIT

stimulates growth promotion

ENHANCEMENT OF EFFICACY

The efficacy of organic acids might be improved…

combining them with phytochemicals!

Phytochemicals Organic acids

PHYTOCHEMICAL. DEFINITION

A term “phytochemical” refers to compounds produced by plants which have protective or preventive properties.

Phytochemicals are found in fruits, vegetables, beans, grains and other plants.

There are more than 1,000 phytochemicals:

Anti-oxidant: allyl sulfides, carotenoids, polyphenols

Antibacterial: allicin, cinnamaldehyde

Hormonal action: isoflavones (soy beans)

IMPROVING EFFICACY OF PRODUCTS

Individual products

Blends of organic acids & phytochemicals

Blend of organic acids, phytochemical & permeabilizers

PERMEABILIZERS. DEFINITION

Permeabilizers weaken the outer membrane (OM) of

Gram-negative bacteria facilitate other antimicrobials to enter

bacterial cells.

BACTERIA STRUCTURE

Outer membrane of Gram-negative bacteria contains lipopolysaccharide layer which makes bacteria resistant to:

• hydrophobic antibiotics,

• detergents,

• bile salts,

• digestive enzymes.

BIOTRONIC® PRODUCT LINE

Active ingredients:

Biomin® Per4izer

Phytochemical

Organic acids

Sequential Release Medium

Biotronic® Top3

BLEND OF ORGANIC ACIDS

Sequential Release Medium

ORGANIC ACIDS. DEFINITION. MODE

OF ACTION

pH reduction

dissociated

H+ R-

undissociated

Antimicrobial effect

RH

H+

H+

H+

ANTIMICROBIAL EFFECT OF ORGANIC

ACIDS

Bacteria is not ableto replicate

•pH reduction uses energy to restore pH

•Disruption of DNA and protein synthesis

•Disruption of membranes

pH 7Intestinal

pH 5-6

(Booth, 1985 – Stratford & Anslow, 1998 - Russel & Diez-Gonzales, 1998 - Roe et al., 1998 – Krebs et al., 1983 – Cherrington et al., 1983 – Freese et al., 1973)

ANTIMICROBIAL EFFECT OF INDIVIDUAL

ORGANIC ACIDS AGAINST SALMONELLA

ANTIMICROBIAL EFFECT OF INDIVIDUAL

ORGANIC ACIDS AGAINST E. COLI

PHYTOCHEMICAL

Sequential Release Medium

CINNAMALDEHYDE

Cinnamaldehyde is the organic compound that gives cinnamon its flavor and odor. It occurs naturally in the bark of cinnamon trees and other species of the genus Cinnamomum.

Cinnamaldehyde plays an essential role in the celldivision reducing the proliferation of potentially harmful bacteria.

PROKARYOTIC CELL DIVISION

Nature Reviews. Molecular cell biology

Cinnamaldehyde

Inhibit FtsZ protein and GTPaseactivity, and assembly into Z-ring.

Bacteria does not divide!

FtsZ = filamentation temperature sensitive protein-Z GTPase = guanosine 5-triphosphate

Domadia et al., 2007

PER4IZER

Sequential Release Medium

INNOVATION BIOMIN® PER4IZER

Biomin® Per4izer is the unique permeabilizing substance identified to enhance the antimicrobial effect of the organic acids andthe phytochemical against pathogenic bacteria.

1. Per4izer weakens gram-negative bacteria outer membrane

2. Per4izer facilitates entry of organic acids and phytochemical

3. Lethal injury of bacteria

IMPROVEMENT OF ANTIMICROBIAL

ACTIVITY BY ADDING PER4IZER

IN VITRO TRIAL WITH BIOTRONIC® TOP3

0

20

40

60

80

100

Blend of organic acids

Biomin® Per4izer

Phytochemical

Sequential Release Medium

CARRIER – SEQUENTIAL RELEASE

MEDIUM

CARRIER

Vermiculite: phyllosilicate/sheet silicate clean to handle odorless mold resistant.

Vermiculite exfoliated expands in a free flowing powder which is able to absorb different substances and serve as carrier.

TRIAL RESULTS IN VIVO

BW vs NC +7%, vs PC +11%

ADWG vs NC +8%, vs PC +16%

FCR vs NC -3%, vs PC -1%

BW vs NC +7%,

ADWG vs NC +8%

FCR vs NC -3%

EFFECT OF BIOTRONIC® TOP3 ON

GROWTH PERFORMANCE IN WEANED PIGS

Negative control Biotronic® Top3 Δ, %

No. animals n = 30 n = 30

Initial body weight, kg 8.75 8.72 /

Final body weight, kg 35.62a 37.69b +6

Feed intake, g/animal 982 1028 +5

Daily weight gain, g 481a 517b +8

FCR 2.04 1.99 -3

Species : weaned piglets (days 28)Duration: 56 dNC = no growth promoterBiotronic® Top3 = 1 kg/t

a,b Means with different superscripts differ significantly; P<0.05

ROI = 11:1Economical benefit 2.5 €/pig

COMMERCIAL TRIAL, CRAILSHEIM, GERMANY

Trial designTreatments:1) Negative control (NC) – no AGP or NGP

2) Treatment – Biotronic® Top3 1 kg/t

90 piglets weaned at 28 days of age/group

Duration of the trial: 56 days

Parameters:

Growth performance (BW, ADWG, FCR)

COMMERCIAL TRIAL, CRAILSHEIM, GERMANY

Results

29.5

30.0

30.5

31.0

31.5

32.0

Control group Biotronic® Top3

group

Body weight (kg), 84 days

30.2

31.5

420

430

440

450

460

Control group Biotronic®

Top3 group

Average daily gain (g)

434

454

1.45

1.5

1.55

1.6

Control group Biotronic® Top3

group

Feed conversion ratio

1.55

1.51

ROI = 9.8:1

4.4%

4.6%

2.6%

SCIENTIFIC TRIAL, HUNAN UNI, CHINA

Trial designTreatments:1) Negative control (NC) – no AGP or NGP

2) Positive control (PC) – Colistin 100g/t + CTC 100g/t

3) Treatment (BTR Top3)– Biotronic® Top3 1 kg/t

Parameters:

Growth performance (BW, ADWG, FCR)

pH value in feed and GIT

Bacteria in GIT

Histology of jejunum

SCIENTIFIC TRIAL, HUNAN UNI, CHINA

Results on growth performanceNC PC BTR

Top3

Δ, %

NC vs BTR

Top3

Δ, %

PC vs BTR

Top3

No. animals n = 32 n = 32 n = 32

Initial BW d35, kg 10.0 10.9 10.7 / /

Final BW d91, kg 42.5a 43.2a 48.1b +13 +11

FI, g/animal/day 1092a 1112a 1269b +16 +14

DWG, g 580a 578ab 668b +15 +16

FCR 1.88 1.92 1.90 +1 -1

Mortality, no ofanimals

1 2 /

a,b Means with different superscripts differ significantly; P<0.05

SCIENTIFIC TRIAL, HUNAN UNI, CHINA

a,b Means with different superscripts differ significantly; P<0.05

Results on pH reduction in feed and GIT

NC PC BTR Top3

pH in feed 5.78 5.73 5.59 0.19

Buffer capacity in feed,

ml of HCl21.7 20.1 13.2 8.5

pH in stomach 4.28a 4.30a 3.67b

0.6

pH in doudenum 5.39 5.35 5.75

pH in jejunum 6.53 6.58 6.42

pH in ileum 6.97 7.02 6.89

pH in colon 6.28 6.52 6.51

SCIENTIFIC TRIAL, HUNAN UNI, CHINA

Results on microbes and morphology of GIT

NC PC BTR Top3

Ileum, Log cfu/g

E. coli 6.302a 5.970b 5.873b

Coliforms 8.277a 7.977b 8.151b

Salmonella typhimurium 8.220a 7.941b 8.053ab

Lactobacillus 6.295a 6.172a 6.515b

Bifidobacteria 6.891a 6.878a 7.130b

Histology of jejunum

Villi length, µm 418.33a 448.63ab 456.18b

Crypt depth,µm 192.37 188.77 187.15

a,b Means with different superscripts differ significantly; P<0.05

CONTRIBUTION TO FEED HYGIENE

Agar diffusion test

The effect of Biotronic® Top3 on the growth inhibition of mold and yeast was evaluated

RESULTS

Strains Inhibition after 96h

Aspergillus fumigatus1 xxx

Fusarium proliferatum x

Candida famata xx

Candida glabrata xx

Candida krusei xx

Fusarium graminearum xxx

Monascus ruber xxx

Penicillium roqueforti x

1Excellent inhibition after 24h

PELLETING TEST

Mixer: Bühler Paddelmixer: Mixing accuracy d =0.00001 Mixing time dry (without fat added): 120 sec* Mixing time in total: 300 sec

Pelletiser: Bühler Serial number 10231288 Type: DPAA 660.178 Die plate: diameter of the openings: 3.5 mm, length 55

mm

Sampling: Samples taken were cooled on plastic plates before

packaging Samples were stored at 4°C until further analysis

PELLETING TEST

Sampling:3 samples of the pre-mixtures in order to determine mixing accuracy

3 samples from the experimental mixture after the mixing process - without any additional treatment

recovery of active ingredients

After steam injection (and before the pelletising process) 3 samples were taken and cooled to room temperature before packaging indication of damaged caused by

the steam injection (hydro thermal stability)

3 samples of feed pellets were taken and cooled to room temperature before packaging indication of damage

caused by steam injection and subsequent pelletisingprocess (hydro thermal-mechanical stability)

RECOVERY OF BIOTRONIC® TOP3 (90°C)

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120

Control Steam heated Pelleted

Flavouring agents

Organic acids

How doesBiotronic® Top3 workwith different antibiotics

CHLORTETRACYCLINE

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120

E. coli O55:K59(B5) E. coli O128:H2 Salmonella enteridits Salmonella typhimurium

In

hib

itio

n,

%

Chlortetracycline Chlortetracycline + Biotronic® Top3

CARBADOX

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120

E. coli O55:K59(B5) E. coli O128:H2 Salmonella enteridits Salmonella typhimurium

In

hib

itio

n,

%

Carbadox Carbadox + Biotronic® Top3

COLISTIN

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120

E. coli O55:K59(B5) E. coli O128:H2 Salmonella enteridits Salmonella typhimurium

In

hib

itio

n,

%

Colistin Colistin + Biotronic® Top3

CONCLUSION

Biotronic® Top3 has no adverse effectson antibiotics

Biotronic® Top3 has an additive inhibitoryeffect effect with some antibiotics on somebacterial strains

Usage of Biotronic® Top3 has no influenceon recommended dosage of antibiotics!

CONCLUSIONS

Dietary supplementation with Biotronic® Top3

Improved growth performance

Lowered pH and buffer capacity in feed

Had an acidification effect in stomach

Decreased pathogenic bacteria

Spared beneficial bacteria

Stimulated growth of villi

Contributed to feed hygiene

APPLICATION

Dose: 1 kg/t of feed for swine

Swine: Pre-starter, starter, fattening

Weaning diarrhea: for optimum results Biotronic® Top3+ a conventional acidifier or probiotics.

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Thank you for your attention!