blood on the shroud of turin: species unknown · 2020. 1. 21. · blood species summary new...
TRANSCRIPT
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Blood on the Shroud of Turin:
Species Unknown
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1970 1980 1990 2000 2010 2020
-
Blood Studies on the Shroud
The very first blood studies on the
Shroud were performed by Frache &
colleagues in the early 1970s
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1970 1980 1990 2000 2010 2020
-
Blood Studies on the Shroud
Their results were negative
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Bloodstained fiber
Test
Solubilization
Aged Bloodstains
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Bloodstained fiber
Test
Solubilization
Aged Bloodstains
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1970 1980 1990 2000 2010 2020
-
Blood Studies on the Shroud
“[The negative answer does not permit an
absolute judgement of the hematic nature of
the material under examination]”
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In 1978 Shroud samples were collected
by:
STURP team
Baima Bollone
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...
Surface tape-lifts
STURP
Collection Methods
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...
Surface tape-lifts Forceps: removal of
certain threads
STURP Baima Bollone
Collection Methods
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...
Surface tape-liftsSTURP
Collection Methods
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...
Surface tape-liftsSTURP
Collection Methods
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Forceps: removal of
certain threads
Baima Bollone
Collection Methods
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To date, this remains the most detailed
investigation of the Shroud bloodstains
Almost all of the reported analyses
of the characterization of the bloodstains
are based on these data
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~
Is blood really present?
The signature molecule for blood
Is Hemoglobin
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lecules
A single RBC contains
~ 250 million Hb molecules
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1970 1980 1990 2000 2010 2020
++
Detection of Hemoglobin (Heme)
Chemical Methods
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1970 1980 1990 2000 2010 2020
+
Detection of Hemoglobin (Heme)
*breakdown products: biliverdin, bilirubin
(Chemical methods)
Spectroscopy Methods
+ +
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What type of blood is it?
Immunological Methods
Species: Animal, Human
Blood type: A, B, AB, O
DNA Methods
Above plus if Male or Female
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The blood on the Shroud is
widely reported as Human in origin
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Immunological Methods
Use specific antibodies as probes
for the molecule of interest
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Whole
Blood
Plasma
Cells
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Whole
Blood
Plasma
Cells
Proteins
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Whole
Blood
Plasma
Cells
Proteins
Albumin
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Whole
Blood
Plasma
Cells
ϒProteins
Albumin
Antibody
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Whole
Blood
Plasma
Cells
ϒProteins
Albumin
Antibody
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Major protein (~55-60%) in blood
Maintains osmotic pressure of extracellular fluid
Carrier protein for many ligands (fatty acids, hormones)
Albumin
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Y
Albumin
Rabbit anti-human albumin antibodies
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Y
Albumin
Rabbit anti-human albumin antibodies
Made in against (specific for)
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Y
Antibody
Immunohistochemistry
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Y
Antibody
Labeled with Fluorescent Tag
or Chemical Tag
Immunohistochemistry
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Y
Antibody
Labeled with Fluorescent Tag
or Chemical Tag
Immunohistochemistry
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Y
Immunohistochemistry
Unstained
fibers
(control)
. ....
.
.
Bloodstained fibers
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Immunochemistry
Control fibers: Negative
Bloodstained fibers: Positive
YY Y
. . ... ..
Results suggest albumin is
present in bloodstained fibers
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What type of blood is it?
Anti-human albumin
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Cross-Reactivity
Human Other Species
Antibodies made against human proteins
may recognize the same protein
found in other species
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Human Other Species
Must be experimentally verified
in separate tests before making any conclusions
Cross-Reactivity
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Y
HumanAlbumin
Rabbit anti-human albumin
Human Albumin
ChimpAlbumin
BaboonAlbumin
CowAlbumin
HorseAlbumin
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Y
HumanAlbumin
Rabbit anti-human albumin
Human Albumin
ChimpAlbumin
BaboonAlbumin
CowAlbumin
HorseAlbumin
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Y
HumanAlbumin
Rabbit anti-human albumin
Human Albumin
ChimpAlbumin
BaboonAlbumin
CowAlbumin
HorseAlbumin
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But the most interesting thing is now there is
immunological evidence that it is primate
blood.”
-Heller & Adler interview 1995
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Y
HumanAlbumin
Rabbit anti-human albumin
Human Albumin
ChimpAlbumin
BaboonAlbumin
CowAlbumin
HorseAlbumin
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Y
HumanAlbumin
Rabbit anti-human albumin
Human Albumin
ChimpAlbumin
BaboonAlbumin
CowAlbumin
HorseAlbumin
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It is now known that albumin from
other animals (not examined)
are very similar to human albumin
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Cross-reactivity
is much greater than previously considered
https://www.cellsignal.com/products/primary-antibodies/albumin-antibody/4929
Luo, et al., Journal of pharmacology and experimental therapeutics”, 321: 884-891 (2007).
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Immunoreactivity of other species is
corroborated by genetic/protein sequencing data
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Cat albumin shows high homology to human albumin
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Whole
Blood
Plasma
Cells
ϒProteins
Albumin
Antibody
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Antibody = Immunoglobulin (Ig)~ 18% total serum protein
IgG
IgM
IgA
IgD
IgE
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Y
Antibody
Labeled with Fluorescent Tag
Immunohistochemistry
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Cross reactivity of anti-human Ig with
other species not addressed/mentioned
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Cross-reactivity with Ig of other species even
more of a concern than with albumin
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Human blood +++
Animal blood +++
Cross-reactivity of Ig
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What type of blood is it?
In 2019, the conclusion
that it has been
demonstrated that human
blood exists on the Shroud
can no longer be justified
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What type of blood is it?
Anatomical accuracy
arguments have their merit
but can only go so far
Direct scientific testing is
required for a definitive answer
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No experiments have
ever been done to
evaluate if animal blood
might be present on the
Shroud
Not part of any
experimental design
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Human blood
Directly verify
Animal blood
Directly rule out
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Blood Species Summary
New evidence shows that cross-reactivity
is much greater than previously considered
These findings are corroborated by
advances in genetic and protein sequencing
of molecules among species
The conclusion that the blood on the Shroud
has been demonstrated to be of human
origin should be reconsidered