bpti: molecular dynamics (300k) · 2012. 4. 26. · bpti: molecular dynamics (300k) a model of...
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![Page 1: BPTI: Molecular Dynamics (300K) · 2012. 4. 26. · BPTI: Molecular Dynamics (300K) A model of Bacteriorhodopsin. BR photocycle. t [ps] C 13 – C 14 C 14 – C 15 ... Gautel et al.,](https://reader035.vdocument.in/reader035/viewer/2022081403/608ac802a39724204f4df7ca/html5/thumbnails/1.jpg)
BPTI: Molecular Dynamics (300K)
![Page 2: BPTI: Molecular Dynamics (300K) · 2012. 4. 26. · BPTI: Molecular Dynamics (300K) A model of Bacteriorhodopsin. BR photocycle. t [ps] C 13 – C 14 C 14 – C 15 ... Gautel et al.,](https://reader035.vdocument.in/reader035/viewer/2022081403/608ac802a39724204f4df7ca/html5/thumbnails/2.jpg)
A model of Bacteriorhodopsin
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BR photocycle
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t [ps]
C13 – C14
C14 – C15
13-14
14-15
NH
H N
Prot.
hν
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W. Zinth et. al.
Time-resolved Pump-Probe Spectrum of Sensorhodopsin
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W. Zinth et. al.
BR J
J K
hν
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FTIR: Spectra from Interferograms
mirror position
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IR spectrum of polystyrene
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IR amide bands of proteins
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Amide I band provides information on secondary structure
![Page 11: BPTI: Molecular Dynamics (300K) · 2012. 4. 26. · BPTI: Molecular Dynamics (300K) A model of Bacteriorhodopsin. BR photocycle. t [ps] C 13 – C 14 C 14 – C 15 ... Gautel et al.,](https://reader035.vdocument.in/reader035/viewer/2022081403/608ac802a39724204f4df7ca/html5/thumbnails/11.jpg)
Monitoring protein unfolding by FTIR: folded
![Page 12: BPTI: Molecular Dynamics (300K) · 2012. 4. 26. · BPTI: Molecular Dynamics (300K) A model of Bacteriorhodopsin. BR photocycle. t [ps] C 13 – C 14 C 14 – C 15 ... Gautel et al.,](https://reader035.vdocument.in/reader035/viewer/2022081403/608ac802a39724204f4df7ca/html5/thumbnails/12.jpg)
Monitoring protein unfolding by FTIR: unfolded
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Difference FTIR
Fritz Siebert
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Time resolved difference FTIR of bacteriorhodopsin
Fritz Siebert
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rupture force
cantilever-position x
force
0
250 pN
Single Molecule Force Spectroscopy
E.-L. Florin, V. T. Moy, H. E. Gaub, Science 264, 415 (1994)
rupture force [pN]co
unts
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Gautel et al., Science 1997, Biophysical Journal 1999Linke and Fernandez, J. of Muscle and Cell Mot. 2002
Force probe experiments (AFM, opt. tweezer):--> Ig domains as “shock absorbers“
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