brochure event
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8/13/2019 Brochure event
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Plant Cytogenetic Experts
The workshop will involve two leading
experts from the Molecular
Cytogenetics Group at the University
of Leicester, UK, who have jointly
published over 300 peer reviewed
articles in the field. This group
specializes in research to understandlarge-scale processes of genome
evolution, including chromosome
rearrangement, genome control,
recombinant, polyploidy, and
epigenetics. Much of their research is
focused on repetitive DNA, including
tandem repeated satellites, DNA
transposons and retrotransposons
involving technology development
work for in situ hybridization, photon
imaging and genomic data analysis. John Seymour (Pat) Heslop-Harrison
Professor of Plant Cell
Biology and Molecular
Cytogenetics at the
University of Leicester,
UK. He is Chief Editor
of Annals of Botany, and
an accepting editor of
Theoretical and Applied
Genetics (TAG) and Plant Reproduction
(formerly Sexual Plant Reproduction).
Trude Schwarzacher She is a co-project leader
of the Molecular
Cytogenetics research
group in the University
of Leicester. She is a
referee for many journals
including Plant Journal,
Chromosoma, Heredity,
Theoretical and Applied Genetics, PNAS,
Euphytica, Hereditas and Annals of Botany.
REGISTRATION FORM
Name
IC number
Gender
University/Institute/Company
Email address
Mailing address
Phone number
All participants are advised to read about FISH and
ChIP at webpages (http://www.molcyt.com and follow
links) before coming to the workshop. Please send registration form by email or fax to
Fax: (603)7967 6991
Email: [email protected] Registration closing date: 20th November 2013
FISH & ChIP
orkshop for plant
genetic study his unique workshop addresses the application
of florescence in situ hybridization (FISH) and
chromatin immunoprecipitation (ChIP) for
studies with plants.
8/13/2019 Brochure event
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FISH
FISH (Fluorescence in situ hybridization) has
become an essential technique in molecular
cytogenetics, involving the combination of
molecular biology and cytogenetics. It can be
used to detect and localize the presences or
absence of specific DNA sequence on
chromosomes. FISH uses fluorescent probes
that bind to only those parts of the
chromosome with which they show a high
degree of sequence complementarity.
Fluorescence microscopy can be used to locate
where the fluorescent probe has bound to the
chromosomes. FISH requires a combination of
skills of molecular biology, cytogenetics,
microscopy, cellular imaging analysis and
immunocytochemistry. The workshop will
provide effective and efficient details of
protocols for FISH applied for plant
cytogenetics.
Co-organized by CEBAR University of Malaya, UMand Malaysia Agro-Biotechnology Institute, ABI
“FISH and ChIP” This three day workshop will
include two main workshopsessions to cover use of each
technique for analysis of plants.
ChIP
ChIP (Chromatin immunoprecipitation) allows
specific protein–DNA interactions to be isolated.
Combining ChIP with high-throughput sequencing
(ChIPseq) reveals the DNA sequence involved in
these interactions. The ChIP technique has been
successfully used in many biological systems to
study histone modifications of
eu/heterochromatin and localization of regulatory
chromatin factors. This method relies on the rapid
cross-linking of protein/DNA complexes within the
nucleus of living cells, followed by chromatinisolation, its random shearing and
immunoprecipitation with antibodies directed
towards proteins of interest. Levels of co-
immunoprecipitated DNA are analyzed by PCR.
The relative enrichment or depletion of a
particular DNA fragment in the
immunoprecipitated fraction reflects its in vivo
association with the examined protein. The
workshop will involve ChIP protocols for plant.
When
17th December 2013 – 19th December 2013 Closing date for registration: 20th November 2013
Where
Malaysia Agro-biotechnology Institute, ABI
Serdang. (Blok A Administrative building)
Registration fee RM 1300 for each participant(Includes tea and lunch each day)
Workshops In depth educational sessions will be given byexperts covering FISH and ChIP techniques andapplications. All participants will get hands-onexperience directed by experts held with thepurpose of demonstrating the techniques orprocesses for both FISH and ChIP. Participants
work with and are exposed to equipment,specimens, chemicals and reagents.
Lecture Series Participants will gain a global perspective on plantscience by attending the opening lecture on the17th Dec 2013 morning session followed byspecialized lecture and discussion sessions,embedded within the workshop for participantsonly.
Contact Us
Centre for Research in Biotechnology for
Agriculture (CEBAR). Level 3, Institute of
Research Management & Monitoring (IPPP),
Research Management & Innovation
Complex, University of Malaya, 50603 Kuala
Lumpur.
Tel: (6017)259 8211
Fax: (603)7967 6991
Email:[email protected]
Website: http://cebar.um.edu.my/