brochure page 1 unipick - neuroindx, inc....universal cell and tissue acquisition system unipick /...

2015

KuiqpicK and UnipicK versatilecell collectionand tissue microdissectionsystems

Single Cell Collection

Tissue microdissection

Transformant Selection

Subculturing

NGS and -Omics applications

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INTRODUCTION

UnipicK /KuiqpicK Cell and Tissue Acquisition Systems TM TM

Collecting individual cells directly from cell cultures for downstream single cell analysis, such astranscriptome sequencing or recultivation purposes, has remained challenging. Similarly, tissueheterogeneity poses a significant challenge for the retrieval of cell and region specific information.Existing approaches, such as flow sorting and laser assisted acquisition, are costly,sophisticated and often methodologically limited.

KuiqpicK™ and UnipicK™ are unique vacuum assisted cell and tissue acquisition systems permittingcutting edge cell specific research at low costs. Our patented vacuum impulse assisted collectionpermits rapid acquisition of single cells from any untreated cell cultures, providing reliable andaffordable means of most sophisticated experimentation. Moreover, the instruments are suitable forindividual cell collection and region specific acquisition of subanatomical areas from complexheterogeneous tissues such as the brain. Operation of both systems is intuitive and requires minimaltraining. Cell cultures require no treatment or specific peripherals, thus enabling unmatched flexibilityin experimental design and sample collection.

Perhaps one of the most distinct features is the ability of both KuiqpicK™ and UnipicK™ tocollect live cells for further reculturing and a plethora of downstream functional studies.Both individual fluorescently labeled and morphologically distinct cells can be acquired fromany cell cultures or tissue sections. Ready-to-use sterile Disposable Capillary Units (DCUs)with variable tip diameter ensure precision of the collection process and minimize contamination.

The instruments’ low cost, ease of use, single cell resolution, and minimal impact on cellviability, make them unique and vital research tools. While KuiqpicK™ is a microscope integratedcell and tissue acquisition system, UnipicK™ is a free standing instrument that can be mountedover a wide range of inverted microscope models, providing additional flexibility in the laboratory.

Notable Features of KuiqpicK and UnipicK include:

- Simple operation with minimal training- Single cell collection from untreated cultures and tissue sections- Efficient tissue microdissection- High viability of collected cells- High quality RNA and protein for downstream –omics studies- Flexibility and versatility - fit any inverted microscope- Fraction of the cost of any laser assisted microdissection instrument


KuiqpicK 1.2TM

KuiqpicKTM is integrated with a fluorescence compatible invertedmicroscope (Olympus CKX41) and provides a wide range of celland tissue acquisition parameters.

Our sample acquisition process does not involve chemicaltreatment, laser irradiation, or excessive heat, thuspreserving the integrity of the macromolecules isolatedfrom the acquired samples.

This integrated system is an essential tool for anyresearch laboratory involved in single cell transcriptomesequencing, cell culturing, tissue region specific research,or single cell studies.

There are countless applications for KuiqpicK.Here are just a few of its capabilities:

Fluorescence-based cell collection: • Collect cells based on fluorescence or in bright field • Collect from highly confluent cultures • Enrich cell lines with low transfection rates using GFP

Transformant enrichment: • Selectively subculture transfected cells • Maintain high cell viability • Maximize efficiency for difficult to transfect cells • Isolate subpopulations from complex mixtures • Achieve high quality separation

Tissue microdissection: • Microdissect sections from 5 to 300 µm thick • Provide minimally invasive environment • Require no pretreatments • Microdissect fresh frozen, sucrose treated and live tissue • Isolate high quality RNA, DNA and protein • Collect individual cells

Universal Cell and Tissue Acquisition System


UnipicK 1.0TM

UnipicK™ 1.0 is a universal system that, in addition to all the benefitsof KuiqpicK™, can be paired with nearly all inverted microscopes.It works in combination with a simple cell culture microscopeor more sophisticated inverted microscope systems.

The greater travel distance of the DCUs provides verticalclearance to work with virtually any cell culture plates.Its notable features include:

• Versatility – collect from cell cultures or tissue slices• Flexibility – able to fit to any inverted microscope • Cost-efficiency – no purchase of additional microscope• Retract Function – easier to work with multiwell plates• Recalibration Function – no recalibration between samples• Side Swivel Head –more comfortable to add and remove DCU

Collection of Single Cells from Adherent Cultures


APPLICATIONS

Most methods for single-cell analysis require large numbersof cells, use workflows that are time consuming and toocostly, and often generate highly variable or unreliable data.

Both KuiqpicK 1.2 and UnipicK 1.0 permit rapid collectionof individual cells from various cell cultures for a range ofdownstream applications including single cell analyses.

Because small sample volume is incremental for singlecell research, KuiqpicK 1.2 and UnipicK 1.0 enable thecollection of a single cell in as small as 12 nl volume.

Profile or subculture cells by quickly and gently collectingup to 25 individual cells per minute from any cell culturedish with no pretreatment. Cells may be collected basedon their morphology or fluorescent label.

Adherent cells can be selectively collected from a standardcell culture dish without the use of Trypsin or other enzymes.Virtually any cell line and cell type can be collected to startselective subcultures. Up to 95% viability rates of collectedcells find numerous applications in a stem cell research,single cell research and many other fields of in vitro cellbased studies.

GFP positive individual CHO cells are collected from standardcell culture dish. This may be used for single cell research,

collection, analysis or enrichment of transfected cells.

AfterBefore

AfterBefore

Representative clonal expansion of collected single CHO cellwithin 1 and 16 days, respectively.

Day 1 Day 16 AfterBefore

Representative collection of a single sperm cellsfrom fixed specimen

AfterBefore

Purkinje Cells, Cerebellum

Before After

Motor neurons, spinal cord

Collection of Single Cells from Brain Tissues and Microdissection


APPLICATIONS

Neuroscience investigators will find both systemsirreplaceable for collecting brain region specific tissuesamples and even individual cells, which can be used for downstream genomics or proteomics studies.

The instruments can microdissect tissue sections withthickness from 10 to 500 µm. Native (live), fresh frozen, andsucrose treated tissues can be used.

High quality of nucleic acids and proteins isolated fromKuiqpicK/UnipicK collected samples ensures flawlessintegration with any –omics workflow including transcriptomesequencing (NGS) and proteomics studies.

Representative collection of single motor neurons andPurkinje cells from the anterior horn of rat spinal

cord and cerebellum, respectively.

Representative mouse brain microdissections (Left), high quality total RNA isolated from microdissected brain tissue samples(RIN >8.5; Middle) and 2D gel (Right) with protein extracts isolated from the dentate gyrus

of adult mouse brain show no signs of degradation.

Ready to use Disposable-Capillary Units (DCU) rangebetween 15 to 100µm internal diameter to maximize

cellular resolution and capturing control.

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Technical Speci�cations


System Performance Ordering Information

Description KuiqpicK 1.1 KuiqpicK 1.2 UnipicK 1.0

Power supply

Input 100-240 V, 50-60 Hz, 1.5 A

Output + 24 V, 2.7 A

Illumination Light source 144 LEDs ring light

Input 24V DC

Vacuum Range up to 22”Hg

Input 24V DC

DCU linear travel/step 1.5 µm

Linear motor input 5 VDC

DCU maximum travel 12.0 mm 23.3 mm

Multiwell compatibility 6-, 12-, 24- 6-, 12-, 24-, 48-, 96-

Microscope TCM400 (Labo America, Inc.) CKX31/41 (Olympus Corp) Range of inverted microscopes

Stage

Size 240 x 160 mm 250 x 160 mm NA

Movement range 114 x 41.3 mm 120 x 78 mm NA

Specimen holder 25 mm slides, Petri dish and multiwall plates

Epi-fluorescence Available from NeuroInDx Available from Olympus NA

Maximum Dimensions & Weight 508 mm (L) x 327.5 mm (W) x 406.5 mm (H), 14.6 kg

371 mm (L) x 441 mm (W) x 473 mm (H), 13.9 kg

523 mm (L) x 456 mm (W) x 507 mm (H), 11.8 kg

Operating Environment Indoor use. Altitude: max. 2000m; Ambient temperature: 5°C to 40°C (41° to 104°F); Maximum relative humidity: 80% for temperature up to 31°C (88°F)

Description Specifications

Vacuum duration (Ts), seconds 0.1 to 1.0 s

Vacuum strength, Hg" 4.4" to 22" Hg

Available DCU IDs, µm From 10 to100 µm

Acquisition speed (Hg"/Ts), seconds

Minimum settings (4.4"/0.1 sec) 1.3 s

Maximum settings (22.0"/1.0 sec) 2.2 s

Acquisition sample volume (Hg"/Ts/DCU ID) *

4.4"/0.1 sec/20 µm 10 to 30 nl

22.0"/1.0 sec/20 µm 1.4 to 2.2 µl

4.4"/0.1 sec/30 µm 35 to 55 nl

22.0"/1.0 sec/30 µm 1.5 to 2.8 µl

4.4"/0.1 sec/40 µm 70 to 100 nl

22.0"/1.0 sec/40 µm 4.7 to 4.9 µl

Cell collection speed (cells/minute) from tissue sections

Rat Purkinje cells (cerebellum) 12.0 ± 1.5 c/min

Mouse anterior horn motor neurons 12.0 ± 1.5 c/min

Cell collection speed (cells/minute) from adherent cell cultures

SH-SY5Y human neuroblastoma cell line Up to 25 c/min

Chinese hamster ovary cells (CHO) Up to 25 c/min

Description Units Catalog Number

KuiqpicK 1.1 with TCM 400 1 NDX-SYST-K101

KuiqpicK 1.2 with CKX31/41 1 NDX-SYST-K102

UnipicK 1.0 Universal System 1 NDX-SYST-U100

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USAFETY INFORMATIONPlease read the instruction manual carefully inorder to safely operate any NeuroInDx product

NeuroInDx, Inc.1655 East 28th StreetSignal Hill, CA 90755

Copyright (c) 2015 NeuroInDx, Inc. All rights reserved. NeuroInDx, the NeuroInDx logo, KuiqpicK and UnipicK are trademarks of NeuroInDx, Inc. in the U.S. and/or other countries. Trademarksbelonging to the third parties are the properties of their respective owners. The manufacture, use and/or sale of NeuroInDx, Inc. product(s) may be subject to one or more patents or pending patentapplications owned by NeuroInDx, Inc. or licensed to NeuroInDx, Inc. from the third parties. Printed in the USA.

For Research Use Only. Not for use in diagnostic procedures.

CONTACT USwww.neuroindx.com [email protected] Tel: 1 562 424 4611 Fax: 1 562 424 4652

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