by : hamidreza sasanfar - european weed research … .pdf · 2015-09-15 · 23-26 june 2015,...
TRANSCRIPT
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17th European Weed Research Society Symposium
23-26 June 2015, Montpellier, France
By : Hamidreza Sasanfar
Ferdowsi University of
Mashhad
Iranian Research Institute of
Plant Protection
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17th EWRS Symposium, 23-26 June 2015, Montpellier, France
Winter wild oat (Avena ludoviciana Durieu.) is the
prevailing Avena species in Iran which is frequently
found at high densities in winter wheat (Triticum
aestivum L.) and barley (Hordeum vulgare L.)
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ACCase inhibitors are a group of herbicides enabling
efficient management of grass weed species such as
wild oat.
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17th EWRS Symposium, 23-26 June 2015, Montpellier, France
Many wild oat populations have been reported to
have become resistant to ACCase inhibitors in the
world.
Seefeldt et al. 1994; Murray et al. 1996; Bourgeois and Morrison 1997; Beckie et al. 2002
But…
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17th EWRS Symposium, 23-26 June 2015, Montpellier, France
Field reports of poor control of grasses led to
investigations on the possibility of resistance evolution in
suspected regions such as Fars and Khuzestan provinces in
Iran.
Zand et al. 2006; Benakashani et al. 2006; Rastgoo et al. 2006
(Heap, 2015)
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To quantify levels of resistance and investigate patterns
of cross-resistance in winter wild oat populations to:
clodinafop, sethoxydim and pinoxaden
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17th EWRS Symposium, 23-26 June 2015, Montpellier, France
It is necessary to detect resistance as early as
possible
management strategies must be developed to prevent
selection and spread of resistance.
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17th EWRS Symposium, 23-26 June 2015, Montpellier, France
Plant Material Collection
13 wild oat (A. ludoviciana)
populations from wheat
fields in Fars province
in 2009
Fars
Population Location Crops planted Herbicide applied
M1 Marv Dasht Wheat-corn Clodinafop (6 yr)
M2 Marv Dasht Wheat-corn-oilseed rape Clodinafop (3 yr), fenoxaprop (2 yr), sethoxydim (1 yr)
M3 Marv Dasht Wheat-corn Clodinafop (4 yr), fenoxaprop (2 yr)
M4 Marv Dasht Wheat-corn Not available
F2 Fasa Wheat-corn Clodinafop (4 yr), fenoxaprop + clodinafop (1 yr), fenoxaprop (1 yr)
F3 Fasa Wheat-corn Clodinafop (4 yr), clodinafop + fenoxaprop (1 yr), fenoxaprop (1 yr)
S1 Sepidan Wheat-corn Not available
S2 Sepidan Wheat-corn-barley-onion Clodinafop (2 yr), fenoxaprop (2 yr), fenoxaprop + difenzoquat (1 yr)
S3 Sepidan Wheat-corn-rice Clodinafop (4 yr), diclofop (2 yr)
S4 Sepidan Wheat-corn Not available
ES Estahban Wheat-corn Clodinafop (2 yr)
ES4 Estahban Wheat-corn-oilseed rape Clodinafop (4 yr), sethoxydim (1 yr)
S Sepidan susceptible population (Seeds were collected from an area with no history of herbicide use)
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17th EWRS Symposium, 23-26 June 2015, Montpellier, France
Plant Material Collection
Sepidan Marvdasht
Estahban Fasa
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17th EWRS Symposium, 23-26 June 2015, Montpellier, France
X
Aryloxyphenoxypropionate(FOPs) clodinafop EC 8% 64 g a.i.
Cyclohexanedione(DIMs) sethoxydim EC 12.5% 375 g a.i.
Phenylpyrazolin(DENs) pinoxaden EC 10% 45 g a.i.
Eight doses : 0, 0.25X, 0.5X, 1X, 2X, 4X, 8X and 16X
Herbicides
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17th EWRS Symposium, 23-26 June 2015, Montpellier, France
Iranian Research Institute of Plant Protection
2010-2011
Seed bioassayWhole plant
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17th EWRS Symposium, 23-26 June 2015, Montpellier, France
Whole-Plant Bioassay
Dose-response experiment in the greenhouse
clodinafop
sethoxydim
pinoxaden
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17th EWRS Symposium, 23-26 June 2015, Montpellier, France
Seed Bioassay
1- Determination of discriminatory concentration
2- Dose response in petri dish
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17th EWRS Symposium, 23-26 June 2015, Montpellier, France
Statistical analysis
Survival, biomass and coleoptiles length
were expressed as a percentage of the
nontreated control .
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Dose-response: Clodinafop
Whole-Plant Bioassay
17th EWRS Symposium, 23-26 June 2015, Montpellier, France
(Marvdasht)
Clodinafop-propargyl (g a.i. ha-1
)
0 16 32 64 128 256 512 1024
Dry
wei
ght (
% o
f non
trea
ted
cont
rol)
0
20
40
60
80
100M1
M2
M3
M4
S
(Fasa)
Clodinafop-propargyl (g a.i. ha-1
)
0 16 32 64 128 256 512 1024
Dry
wei
ght (
% o
f non
trea
ted
cont
rol)
0
20
40
60
80
100 F2
F3
S
(Sepidan)
Clodinafop-propargyl (g a.i. ha-1
)
0 16 32 64 128 256 512 1024
Dry
wei
ght (
% o
f non
trea
ted
cont
rol)
0
20
40
60
80
100 S1
S2
S3
S4
S
(Estahban)
Clodinafop-propargyl (g a.i. ha-1
)
0 16 32 64 128 256 512 1024
Dry
wei
ght (
% o
f non
trea
ted
cont
rol)
0
20
40
60
80
100 ES4
ES
S
15
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Whole-Plant Bioassay
1617th EWRS Symposium, 23-26 June 2015, Montpellier, France
S (Susceptible biotype)
M2 (The highest resistant biotype)
Control 0.25 X 16 X8 X4 X2 X1 X0.5 X
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Dose-response: Sethoxydim
Whole-Plant Bioassay
17th EWRS Symposium, 23-26 June 2015, Montpellier, France
(Marvdasht)
Sethoxydim (g a.i. ha-1
)
0 94 188 375 750 1500 3000 6000
Dry
wei
ght (
% o
f non
trea
ted
cont
rol)
0
20
40
60
80
100 M1
M2
M3
M4
S
(Fasa)
Sethoxydim (g a.i. ha-1
)
0 94 188 375 750 1500 3000 6000
Dry
wei
ght (
% o
f non
trea
ted
cont
rol)
0
20
40
60
80
100 F2
F3
S
(Sepidan)
Sethoxydim (g a.i. ha-1
)
0 94 188 375 750 1500 3000 6000
Dry
wei
ght (
% o
f non
trea
ted
cont
rol)
0
20
40
60
80
100 S1
S2
S3
S4
S
(Estahbanا)
Sethoxydim (g a.i. ha-1
)
0 94 188 375 750 1500 3000 6000
Dry
wei
ght (
% o
f non
trea
ted
cont
rol)
0
20
40
60
80
100 ES4
ES
S
17
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Control 0.25 X 16 X8 X4 X2 X0.5 X 1 X
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17th EWRS Symposium, 23-26 June 2015, Montpellier, France
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Dose-response: Pinoxaden
Whole-Plant Bioassay
17th EWRS Symposium, 23-26 June 2015, Montpellier, France
(Marvdasht)
Pinoxaden (g a.i. ha-1
)
0 11 23 45 90 180 360 720
Dry
wei
ght (
% o
f non
trea
ted
cont
rol)
0
20
40
60
80
100 M1
M2
M3
M4
S
(Fasa)
Pinoxaden (g a.i. ha-1
)
0 11 23 45 90 180 360 720
Dry
wei
ght (
% o
f non
trea
ted
cont
rol)
0
20
40
60
80
100
120 F2
F3
S
(Estahban)
Pinoxaden (g a.i. ha-1
)
0 11 23 45 90 180 360 720
Dry
wei
ght (
% o
f non
trea
ted
cont
rol)
0
20
40
60
80
100 ES4
ES
S
(Sepidan)
Pinoxaden (g a.i. ha-1
)
0 11 23 45 90 180 360 720
Dry
wei
ght (
% o
f non
trea
ted
cont
rol)
0
20
40
60
80
100
120 S1
S2
S3
S4
S
19
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Dose-response: Pinoxaden
Whole-Plant Bioassay
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17th EWRS Symposium, 23-26 June 2015, Montpellier, France
M2
S4
0.25 X 0.5 X 1 X
4 X2 X1 X
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17th EWRS Symposium, 23-26 June 2015, Montpellier, France
Table 1 . The estimated herbicide rate required for 50% reduction (ED50) in shoot dry
matter of 12 resistant and one susceptible winter wild oat populations and their
corresponding resistance ratios (R/S) to three ACCase inhibitor herbicides
Clodinafop Sethoxydim Pinoxaden
Population
ED50 z
(g ai L-1)
R/S
ED50
(g ai L-1)
R/S
ED50
(g ai L-1)
R/S
M1 74.6 7.6 131.8 2.1 43.6 6.4
M2 >336.26 y >34.1 170.7 2.7 1.9 0.3
M3 >336.26 >34.1 201.5 3.2 43.9 6.5
M4 336.26 34.1 305.4 4.9 6.1 0.9
F2 30.9 3.1 672.1 10.7 123.1 18.1
F3 204.7 20.7 454.5 7.3 24.3 3.6
S1 66.8 6.8 107.4 1.7 6.9 1.0
S2 41.2 4.2 413.5 6.6 188.6 27.8
S3 105.8 10.7 145.8 2.3 15.0 2.2
S4 67.2 6.8 385.2 6.1 182.4 26.9
ES 15.1 1.5 73.5 1.2 2.6 0.4
ES4 38.1 3.9 762.5 12.2 123.6 18.2
S x 9.9 1.0 62.7 1.0 6.8 1.0
z ED50 values were estimated from the log-logistic model (Eq1).
y Shoot dry weight reductions were less than 50% over the range of clodinafop
application rates, thus the ED50 was not estimable.
x Susceptible population had never been treated with any of herbicides tested.
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17th EWRS Symposium, 23-26 June 2015, Montpellier, France
Dose-response: Clodinafop
Seed Bioassay
(Marvdasht)
Clodinafop-propargyl (mg a.i. L-1
)
0.00 0.08 0.16 0.32 0.64 1.28 2.56 5.12
Col
eopt
iles l
engt
h (%
of n
ontr
eate
d co
ntro
l)
0
20
40
60
80
100
M1
M2
M3
M4
S
(Fasa)
Clodinafop-propargyl (mg a.i. L-1
)
0.00 0.08 0.16 0.32 0.64 1.28 2.56 5.12
Col
eopt
iles l
engt
h (%
of n
ontr
eate
d co
ntro
l)
0
20
40
60
80
100 F2
F3
S
(Estahbanا)
Clodinafop-propargyl (mg a.i. L-1
)
0.00 0.08 0.16 0.32 0.64 1.28 2.56 5.12
Col
eopt
iles l
engt
h (%
of n
ontr
eate
d co
ntro
l)
0
20
40
60
80
100 ES
ES4
S
(Sepidan)
Clodinafop-propargyl (mg a.i. L-1
)
0.00 0.08 0.16 0.32 0.64 1.28 2.56 5.12
Col
eopt
iles l
engt
h (%
of n
ontr
eate
d co
ntro
l)
0
20
40
60
80
100 S1
S2
S3
S4
S
22
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17th EWRS Symposium, 23-26 June 2015, Montpellier, France
Dose-response: Clodinafop
Seed Bioassay
S
Control 0.25 X 16 X8 X4 X2 X1 X0.5 X
M2
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17th EWRS Symposium, 23-26 June 2015, Montpellier, France
Dose-response: Sethoxydim
Seed Bioassay
(Marvdasht)
Sethoxydim (mg a.i. L-1
)
0.0 0.5 1.0 2.0 4.0 8.0 16.0 32.0
Col
eopt
iles l
engt
h (%
of n
ontr
eate
d co
ntro
l)
0
20
40
60
80
100 M1
M2
M3
M4
S
(Fasa)
Sethoxydim (mg a.i. L-1
)
0.0 0.5 1.0 2.0 4.0 8.0 16.0 32.0
Col
eopt
iles l
engt
h (%
of n
ontr
eate
d co
ntro
l)
0
20
40
60
80
100
120 F2
F3
S
(Estahban)
Sethoxydim (mg a.i. L-1
)
0.0 0.5 1.0 2.0 4.0 8.0 16.0 32.0
Col
eopt
iles l
engt
h (%
of n
ontr
eate
d co
ntro
l)
0
20
40
60
80
100 ES
ES4
S
(Sepidan)
Sethoxydim (mg a.i. L-1
)
0.0 0.5 1.0 2.0 4.0 8.0 16.0 32.0
Col
eopt
iles l
engt
h (%
of n
ontr
eate
d co
ntro
l)
0
20
40
60
80
100 S1
S2
S3
S4
S
24
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17th EWRS Symposium, 23-26 June 2015, Montpellier, France
Dose-response: Pinoxaden
Seed Bioassay
(Marvdasht)
Pinoxaden (mg a.i. L-1
)
0.00 0.10 0.20 0.40 0.80 1.60 3.20 6.40
Col
eopt
iles l
engt
h (%
of n
ontr
eate
d co
ntro
l)
0
20
40
60
80
100 M1
M2
M3
M4
S
(Fasa)
Pinoxaden (mg a.i. L-1
)
0.00 0.10 0.20 0.40 0.80 1.60 3.20 6.40
Col
eopt
iles l
engt
h (%
of n
ontr
eate
d co
ntro
l)
0
20
40
60
80
100 F2
F3
S
(Estahban)
Pinoxaden (mg a.i. L-1
)
0.00 0.10 0.20 0.40 0.80 1.60 3.20 6.40
Col
eopt
iles l
engt
h (%
of n
ontr
eate
d co
ntro
l)
0
20
40
60
80
100 ES
ES4
S
(Sepidan)
Pinoxaden (mg a.i. L-1
)
0.00 0.10 0.20 0.40 0.80 1.60 3.20 6.40
Col
eopt
iles l
engt
h (%
of n
ontr
eate
d co
ntro
l)
0
20
40
60
80
100
120 S1
S2
S3
S4
S
25
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17th EWRS Symposium, 23-26 June 2015, Montpellier, France
Dose-response: Pinoxaden
Seed Bioassay
M2
Control 1 X 2 X
4 X 8 X 16 X
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17th EWRS Symposium, 23-26 June 2015, Montpellier, France
Seed Bioassay
Table 2. The estimated herbicide rate required for 50% reduction (ED50) in coleoptiles
elongation of 12 resistant and one susceptible winter wild oat populations and their
corresponding resistance ratios (R/S) to three ACCase inhibitor herbicides
z ED50 values were estimated from the log-logistic model (Eq. 1).
y Plant survival was higher than 50% over the range of clodinafop application rates, thus
the ED50 was not estimable.
x Susceptible population had never been treated with any of herbicides tested.
Clodinafop Sethoxydim Pinoxaden
Population
ED50 z
(mg ai L-1)
R/S
ED50
(mg ai L-1)
R/S
ED50
(mg ai L-1)
R/S
M1 0.95 12.83 0.53 1.29 0.64 4.06
M2 >1.95 y >26.3 0.49 1.19 0.37 2.36
M3 1.38 18.57 0.42 1.01 1.83 11.58
M4 0.55 7.48 1.03 2.52 0.50 3.16
F2 0.70 9.49 1.18 2.88 1.64 10.38
F3 1.21 16.39 6.43 15.70 1.08 6.84
S1 1.95 26.26 0.44 1.07 0.55 3.46
S2 0.50 6.73 1.04 2.54 5.09 32.21
S3 0.30 4.06 0.31 0.76 1.73 10.95
S4 0.37 4.93 0.58 1.41 2.50 15.86
ES 0.05 0.70 0.26 0.65 0.29 1.83
ES4 0.61 8.16 1.28 3.12 3.32 21.03
S x 0.07 1.00 0.41 1.00 0.16 1.00
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17th EWRS Symposium, 23-26 June 2015, Montpellier, France
DENsDIMsFOPsPopulation
*****M1
-*****M2
*******M3
-*****M4
*******F2
*******F3
-***S1
*******S2
*****S3
********S4
-**ES
*******ES4
---S
*, **, *** and **** showed resistance index (RI) <5, 5-10, 11-20 and >20, respectively.
Patterns of cross-resistance
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17th EWRS Symposium, 23-26 June 2015, Montpellier, France
The selective pressure from other ACCase inhibitors can
result in cross-resistance to pinoxaden.
A pinoxaden's phenylpyrazolin chemistry and proposed
different ACCase binding domains can not necessarily
indispose the risk of pinoxaden resistance.
The resistance levels of most population to clodinafop
were considerably higher than those to sethoxydim.
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European Weed Research Society
Ferdowsi University of Mashhad
Hamidreza SasanfarDepartment of Agronomy,
Ferdowsi University of Mashhad, [email protected]
17th EWRS Symposium, 23-26 June 2015, Montpellier, France