by: samantha cooney and joshua mckain. water quality differences are known to affect macro...
TRANSCRIPT
By: Samantha Cooney and Joshua McKain
Phosphate Levels and Macro Invertebrate
Diversity
Water quality differences are known to affect macro invertebrate diversity
Phosphate levels are known to spike in Fernan Lake
Could disequilibrium between phosphorus and nitrogen in the lake affect macro invertebrate diversity in lake outlet?
Justification
Initial QuestionDo high phosphate concentrations affect macro
invertebrate biodiversity in riffles?
HypothesisIf phosphate levels are high, macro
invertebrate biodiversity will be low.
Introduction
Disturb a .5 square meter area riffle with shovel
Collect macro inverts with a kick netOrganize inverts based on high quality,
medium quality, and low quality speciesCollect pH, DO, water temp., air temp, and
phosphorous concentration at invert collection sites
Draw correlations using Anova
Methods
ShovelKick net4 sample tubesLab quest Probes: DO, pH, tempBug viewerHach kitForcepsDichotomous keys
Materials
Site 1 and 2
Site 4 and 5
Site 3
Site 1
Site 2
Site 3
Site 4
Site 5
Location Air Temp (°C) Water Temp (°C) DO (mg/L) "Phosphorus" pH
1 18 15 8.92 2 7.8
2 14 14.2 9.33 2 7.6
3 20 8.5 7.9 3 7.8
4 19 10.4 7.7 2 7.7
5 20 9.3 9.5 0 7.5
AVERAGE 18.2 11.48 8.67 1.8 7.68
variance 6.2 8.647 0.6802 1.2 0.017
sd 2.48998 2.940578 0.824742 1.095445 0.130384
Results
LocationHigh Quality
Species # SpeciesModerate Quality
Species # SpeciesLow Quality
Species # Species
1 2 2 5 4 1 1
2 0 0 5 3 3 4
3 1 1 3 2 3 3
4 1 2 0 0 1 2
5 5 7 1 1 3 3
Results
Site 4 had low diversity and pop. density because there was no water flowing the day before we collected our samples.
Anova: Single Factor
SUMMARYGroups Count Sum Average Variance
phosphor 5 9 1.8 1.2high quality species 5 12 2.4 7.3
ANOVASource
of Variation SS df MS F P-value F critBetween Groups 0.9 1 0.9
0.211765
0.657637
5.317655
Within Groups 34 8 4.25
Total 34.9 9
Analysis of the Variance to find if the means are correlated(Ho: µ1=µ2) (Ha: µ1≠µ2). • f* is not in the critical region, there for we
accept (Ho: µ1=µ2) and reject the null hypothesis (Ha) that the means are not related.
• P-value 0.657637> 0.05 therefore we accept Ho
Mirror Adventure LearningPlace-based learning and field trips
More time collecting data on invertsLessons on dichotomous keys
Blogging AL vs. DL
Application in the classroom
Data supported hypothesisPhosphate concentrations seem to have an
affect on macro invertebrate diversityDifference of means are related
Too little data may have skewed resultsMore time in data collectionMore time identifying individual speciesConfusion with units for phosphate
Conclusion