c-more hoe dylan vii 5-14 august 2012hahana.soest.hawaii.edu/hoedylan/documents/hoe-dylan-vii...otg...
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C-MORE HOE DYLAN VII 5-14 August 2012 Draft cruise plan
Cruise ID: KM12-17 Vessel: R/V KILO MOANA University of Hawaii Master of the Vessel: Captain Rick Meyer Chief Scientist: Sonya Dyhrman (WHOI) and Paul Berube (MIT) OTG Marine Technicians: Ben Colello and Dave Hashisaka Shipping address: Blake Watkins C-MORE 5-14 Aug 2012, KM12-17 C/O UH Marine Center #1 Sand Island Access Road Honolulu, HI 96819 Kilo Moana phone number: 842-9817, cell 864-0065, satellite 001-870-336-956510 Marine Center phone number: 842-9813 Sonya Dyhrman: 253-677-7117 Paul Berube: 206-403-8082 Loading: Aug 4, 2012 @ 0800 Departure: Aug 5, 2012 @ 0900 Arrival: Aug 14, 2012 @ 0800 1.0 SCIENTIFIC OBJECTIVES The objective of the cruise is to maintain a collection of hydrographic and biogeochemical data at the Hawaii Ocean Time-series (HOT) station. Station ALOHA, is defined as a circle with a 6 nautical mile radius centered at 22° 45'N, 158°W. This is the main HOT station and will be occupied throughout the cruise. The summary schedule is as follows: 4 August Ship loading at 0800. 5 August Depart from Snug harbor at 0900. Science personnel on-board by 0800. 5-14 August Station ALOHA operations. 14 August Arrive back to Snug Harbor. Full offload. 2.0 SCIENTIFIC PERSONNEL Participant Title Affiliation Samar Abedrabbo (F) Grad Student UCSC Harriet Alexander (F) Grad Student WHOI Lydia Baker (F) Grad Student UH/CMORE Benedetto Barone (M) Post-Doc UH/CMORE Paul Berube (M) Post-Doc MIT
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Karin Björkman (F) Research Specialist UH/CMORE Mitchell Chong (M) Research Assistant UH/CMORE Tara Clemente (F) Research Associate UH/CMORE Ken Doggett (M) Research Associate UH/CMORE Sonya Dyhrman (F) Faculty WHOI Rosie Gradoville (F) Grad Student OSU Rick Kayser (M) Research Specialist MIT Ricardo Letelier (M) Faculty OSU Vanessa Michelou (F) Post-Doc UH/CMORE Jackie Mueller (F) Grad Student UH/CMORE Abigail Noble (F) Post-Doc MIT Cory Padilla (M) Research Assistant UH/CMORE Nicole Pereira (F) Grad Student UCSC Monica Rouco-Molina (F) Post-Doc WHOI Sara Thomas (F) Grad Student UH/CMORE Angel White (F) Faculty OSU Ben Colello OTG Tech Marine Center Dave Hashisaka OTG Tech Marine Center
21 Scientists (14 Female, 7 Male) + 2 OTG 3.0 LABORATORY AND INCUBATOR SPACE 3.1. Laboratory Space Hydro Lab: Tara Clemente, Karin Björkman, Cory Padilla, Mitchell Chong, Sara Thomas. Chem Lab: Samar Abedrabbo, Nicole Pereira, Jackie Mueller. Lab #2 (board side): Rick Kayser, Abigail Noble, Angel White, Ricardo Letelier, Rosie Gradoville, Benedetto Barone. Lab #2 (outboard side): Lydia Baker, Sonya Dyhrman, Monica Rouco-Molina, Harriet Alexander, Vanessa Michelou, Paul Berube. IMET Lab (forward section): Available 3.2. Van Space Flow Cytometry: Ken Doggett OTG Rad Van: Angel White Van (#23): Karin Björkman and Sara Thomas 3.3. Incubator Space OSU incubations are in progress and continuing from HOE DYLAN V and will be sampled by Angel White. Dyhrman Lab will set up 18 X 20-L bottles in three large UH
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on-deck incubators (deck locations TBD) – they will be occupied for the whole cruise. Nicole Pereira will be running 2 x 30 hour incubations in the small Zehr Lab incubators with 60L water each from two 1700hrs casts (deck locations TBD). Sara Thomas will require 1 of the UH on deck incubators and she is planning to bring a chest freezer sized incubator that would need to go into the staging bay on the KM (she will have an incubation continuing from the previous HOT cruise in this incubator). 4.0 OPERATIONAL PLANS Shipboard operations: CTD operations; primary productivity array; optics casts; trace metal sampling; misc. experiments. Underway/continuous operations: ADCP, thermosalinograph, pCO2 system, fluorometry, and meteorology. Whenever pumping of the ship’s tanks is needed, it must be conducted outside the circle that defines station ALOHA (Sect. 1.0). To avoid disruptions in the schedule, this operation should be coordinated with the chief scientists and we have allocated three times a day when this operation can occur. 4.1. Water column measurements Vertical profiles of temperature, conductivity and dissolved oxygen will be made with an instrument package consisting of a Sea-Bird CTD attached to a 24-place rosette with 12 liter Bullister sampling bottles. We will need the ship’s CTD winch and crane for these operations. Water samples for biogeochemical measurements will be collected on each cast. There will be an additional instrument to be included with the OTG CTD package – an ISUS which is a real time, chemical free sensor for measuring nitrate. Its power requirement is 0.625 amps at 12V. Furthermore we wish to mount a lowered ADCP to the rosette frame. The lowered ADCP is self-contained and does not need integration with the CTD. It has a mounting that puts it at the centre of the CTD frame. 4.2. Pump sampling In addition to sampling the water-column using the CTD-rosette, we will also use Dan’s diaphragm water pumping systems. It will be installed and deployed at 25m. Flow rate is roughly 4L/min. 4.3. Automated Trace Element Sampler (ATE) The ATE will be hand-deployed daily off the back deck to a depth of 10 m. The ATE will be recovered after 30 minutes in the water. The ATE is approximately 1’ tall and 4’’ in diameter, weighting 5 lbs. 4.4. VANE sampling Trace-metal clean samples will be collected once using VANE samplers attached to the 0.322 hydrowire. Depths are base of ML, DCM, 50-70m below DCM, 250, 375, 500, 750, 1000, 1200 (hydrothermal maximum), and 1400 [max depth of cast is 1500m].
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4.5. Hyperpro Daily deployment of Satlantic radiometer to characterize irradiance and radiance. The Hyperpro is a profiling unit with one up-looking and one down-looking hyperspectral radiometer, a WET Labs ECO-BB2F triplet (measuring Chlorophyll-a fluorescence and backscattering in the blue and red wavelengths), temperature and conductivity sensors. This instrument also incorporates a ship mounted surface radiometer. The Hyperpro will be deployed from the stern through a small block hung from the A-frame. The instrument is hand-lowered and retrieved with assistance from the winch. 4.6. ACS/AC9/FRRf/LISST An optical package including a Wet Labs AC9 that measures water column spectral absorption and attenuation at nine wavelengths, a Chelsea Fast Repetition Rate Fluorometer (FRRf), a SeaBird Seacat with temperature, conductivity, fluorometer, and pressure sensors, and a LISST particle size and distribution analyzer will be deployed two times during the cruise. Each deployment will consist of two up and two down profiles to a target depth of 200 m at a constant speed of 10 m/min during both the downcast and upcast. An instrument soaking period at just below the surface will be required between the two profiles. The A-frame and capstan will be needed for this operation. 4.7. Primary production experiment Samples for the primary productivity experiment will be collected from the rosette. Before dawn (Sunrise 0606 hrs on August 8th), a free drifting incubation array will be deployed from the stern using the A-frame and Sea-Mac winch. The array is equipped with two ARGOS satellite transmitters (platform #’s 3028, 60482, emailing positions to [email protected], password: argosfix), strobe lights and a radio transmitter (156.425 MHz). The ship shall keep within site of the array while performing CTD operations for the last 6 hours of the approximately 12-hour time the array will be in the water unless the array drifts outside of the ALOHA circle. If the array drifts out of the circle, the ship should return inside the circle to conduct CTD casts, and the monitoring of the array will be coordinated with the watch leader. All radioactive waste generated by the experiment shall be returned to the University of Hawaii. Only qualified personnel shall handle radioactive material. 4.8. Acoustic Doppler Current Profiler The ship’s acoustic Doppler current profiler (ADCP) will be in operation during the duration of the cruise. The OTG technicians will be in charge of the ADCP system. 4.9. Thermosalinograph, pCO2 system, Fluorometer, and meteorological system The ship’s thermosalinograph, pCO2 system and fluorometer sampling the uncontaminated seawater supply system will be in operation during the duration of the cruise while the ship is outside of Snug Harbor. The ship’s meteorological system shall be in operation throughout the cruise. Access to real-time underway data through the ship’s network will be required. The OTG technicians will be in charge of the thermosalinograph, pCO2 system, Fluorometer, and meteorological suite operations.
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4.10. We also request that OTG group initiate the shell script to automatically send the ships coordinates approximately hourly by email to [email protected] and [email protected]. This will be important to cross-calibrate the instrumentation in the water (sea-gliders-floats). If possible, it can be left running from July-September as it would be useful on the HOT legs as well. 5.0. EQUIPMENT 5.1 The science party shall be bringing the following
1. One 20 ft. laboratory van (Karl Flow Cytometry) 2. One 20 ft. laboratory van (#23) with assorted equipment for radioisotope and
general use 3. One 20 ft. OTG rad van 4. All required chemicals and isotopes 5. Large vacuum waste containers 6. Liquid nitrogen dewar 7. Polypropylene line 8. Drifting primary production array with light and radio transmitter, floats, weights,
polypropylene line, spare buoy, etc. 9. Hyperpro and other optical measuring instruments. 10. Oxygen titration system 11. Plankton nets and towing lines 12. Desktop and laptop personal computers 13. Assorted tools 14. All required sampling bottles 15. Deck incubation system 16. Pertinent MSDS 17. Trace metal clean sampling devices including ATE sampler and VANES 18. Desktop and laptop personal computers
5.2 We will need the use of the following ship's equipment:
1. OTG’s 24-place rosette, and 24 12-l water sampling bottles 2. CTD Instrument package 3. A-frame 4. A-frame block assembly 5. Caley winch and crane with conducting wire for CTD 6. Electric power for winches (440 VAC, 3 phase, 60 Amp breaker) and vans (208
VAC single phase at 60 amps for lab van, 110 VAC 10 amps for equipment van) 7. Radio direction finder 8. Space on the upper deck for two 20ft. laboratory vans (Karl FCM Van and the
OTG Rad Van) 9. Space on main deck for one 20ft. laboratory van (#23) 10. Space on upper deck for incubators 11. Hand-held VHF transceivers 12. Precision depth recorder
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13. Shackles, sheaves, hooks and lines 14. Shipboard Acoustic Doppler Current Profiler 15. Thermosalinograph, pCO2 systen, and Fluorometer 16. Meteorological suite 17. Copy machine 18. Grappling hooks and line 19. Laptop with Nobeltec charting software and GPS feed 20. Running fresh water and seawater, hoses 21. Electronic mail system 22. GPS system 23. Uncontaminated seawater supply 24. Small capstan (~ 10 m/min) 25. Underway/on-station data acquisition system for meteorological instruments,
ADCP, 26. Thermosalinograph, fluorometer, pCO2 and access to real-time data through the
network 27. OTG’s 24-place rosette, and 24 12-l water sampling bottles (to be used as spare) 28. 1000 lb weight 29. Large Sea-Mac winch (Mod. 1025 EHS). 60 Amp Hubbel plug/connector (440
VAC, 3 phase, 60 Amp breaker) 30. Kevlar line
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Ship: R/V KILO MOANA KM12-17 Date: 5-14 August, 2012 TIME Sat. 8/4 Sun. 8/5 Mon. 8/6 Tues. 8/7 Wed. 8/8 0000 CTD cast CTD cast CTD cast
0100
0200
0300
0400 Rate cast Rate cast Rate cast
0500 Deploy PP array
0600
0700 Loading for DYLAN 7
Scientists onboard ATE sample (30 mins)
ATE sample (30 mins)
ATE sample (30 mins)
0800 CTD cast CTD cast CTD cast
0900 Transit ALOHA
1000 Optics Optics Optics
1100
1200 Isopycnal Cast
Isopycnal Cast Isopycnal Cast
1300 Hyperpro
Hyperpro Hyperpro
1400 Diaphragm pump Net tow CTD – Large vol
1500
1600
1700 Biogeochem Cast CTD cast CTD cast
1800
1900 Arrive ALOHA Recover PP array
2000 CTD cast CTD cast CTD cast CTD cast
2100
2200 Optics
Optics
Optics
2300
August 6th: Sunrise 0606, Sunset 1910
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Ship: R/V KILO MOANA KM12-17 Date: 5-14 August, 2012 TIME Thurs. 8/9 Fri. 8/10 Sat. 8/11 Sun. 8/12 Mon. 8/13 0000 CTD cast CTD cast
Burst sampling CTD cast Burst sampling
CTD cast CTD cast (1500 dbar)
0100
0200
0300 Vanes (1500 dbar)
0400 Rate cast Rate cast Burst sampling
Rate cast Burst sampling
Rate cast
0500
0600
0700 ATE sample (30 mins)
ATE sample (30 mins)
ATE sample (30 mins)
ATE sample (30 mins)
ATE sample (30 mins)
0800 CTD cast CTD cast Burst sampling
CTD cast Burst sampling
CTD cast CTD cast
0900
1000 Optics Optics Optics Optics Optics
1100
1200 Isopycnal Cast
Isopycnal Cast Burst sampling
Isopycnal Cast Burst sampling
Isopycnal Cast
Isopycnal Cast
1300 Hyperpro
Hyperpro
Hyperpro
Hyperpro
Hyperpro
1400 Net tow CTD – Large vol Net tow CTD – Large vol
1500 Transit to Snug
1600
1700 Biogeochem Cast CTD cast Burst sampling
CTD cast Burst sampling
Biogeochem Cast
1800
1900
2000 CTD cast CTD cast Burst sampling
CTD cast Burst sampling
CTD cast
2100
2200 Optics
Optics
Optics
Optics
2300
August 11th: Sunrise 0608, Sunset 1906
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Ship: R/V KILO MOANA KM12-17 Date: 5-14 August, 2012 TIME Tues. 8/14 Wed. 8/15 Thurs. 8/16 Fri. 8/17 Sat. 8/18 0000
0100
0200
0300
0400
0500
0600
0700 Arrival at Snug and Offload
Loading for HOT245
Scientists onboard for HOT245
0800
0900 Ship departs for HOT245
1000
1100
1200
1300
1400
1500
1600
1700
1800
1900
2000
2100
2200
2300 August 16th: Sunrise 0609, Sunset 1903
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Ship: R/V KILO MOANA KM12-17 CTD CASTS Date: August 5-14, 2012 Day Cast Time Max
Depth Samples Bottles
August 5 s2c1 2000 500m KD (1 @ 75, 100, 110, 115, 120, 125, 130, 135, 140, 145, 150, 175); RG (5 @ 5m); LB (5 bottles)
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August 6 s2c2 0000 400m 0
s2c3 0400 700m rate cast: ST (4 @ 25); SD, MRM, HA (3 @ 700m and 1 @ 5, 25, 45,75,100, 400); AW (2 @ 25); LB (5 bottles)
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s2c4 0800 1000m Karl (1 @ 5, 100, 200, 300, 500, 750, 900, 1000); Zehr (1 @ 5, 25, 45, 75, 100, 125, 150, 175); Church (1 @ 5, 25, 45, 75, 100, 125); O2Ar-Karl (1 @ 5, 25, 45, 75, 100); KB (1 @ 25)
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s2c5 1200 400m isopycnal cast: (3 @ 25, 75, 100, DCM, DCM isopyc, NO2 max, 175, 250)
24
s2c6 1700 400m biogeochem cast: (3 @ 5, 25, 45, 75, 100, 125, 150, 175)
24
s2c7 2000 400m LB (5 bottles) 5
August 7 s2c8 0000 400m Zehr (1 @ 25m, 45m) 2
s2c9 0400 400m rate cast: ST (4 @ 25); SD, MRM, HA (1 @ 5, 25, 45,75,100, 400); AW (4 @ 25); LB (5 bottles); Zehr (1 @ 25, DCM)
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s2c10 0800 1000m Karl (1 @ 5, 100, 200, 300, 500, 750, 900, 1000); Zehr (1 @ 25m, 45m); Church (1 @ 5, 25, 45, 75, 100, 125); O2Ar-Karl (1 @ 5, 25, 45, 75, 100); KB (1 @ 25)
15
s2c11 1200 400m isopycnal cast: (3 @ 25, 75, 100, DCM, DCM isopyc, NO2 max, 175, 250)
24
s2c12 1700 400m Zehr (1 @ 25, DCM); NP (9 @ 5m); JM (3 @ 25, 75, 125)
14
s2c13 2000 400m LB (5 bottles) 5
August 8 s2c14 0000 400m 0
s2c15 0400 400m rate cast: ST (4 @ 25); AW (2 @ 25); LB (5 bottles); PParray (3 @ 5, 25, 45, 75, 100, 125); KD (1 @ 5, 25, 45, 75, 100, 125)
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s2c16 0800 1000m Karl (1 @ 5, 100, 200, 300, 500, 750, 900, 1000); Zehr (1 @ 5, 25, 45, 75, 100, 125, 150, 175); Church (1 @ 5, 25, 45, 75, 100, 125); O2Ar-Karl (1 @ 5, 25, 45, 75, 100); KB (1 @ 25)
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s2c17 1200 400m isopycnal cast: (3 @ 25, 75, 100, DCM, DCM isopyc, NO2 max, 175, 250)
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s2c18 1400 400m SD/MRM/HA 12 @ 25m 12
s2c19 1700 400m Zehr (1 @ 25, DCM); JM (3 @ 25, 75, 125) 5
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s2c20 2000 400m LB (5 bottles) 5
August 9 s2c21 0000 400m Zehr (1 @ 25m, 45m) 2
s2c22 0400 400m rate cast: ST (4 @ 25); SD, MRM, HA (1 @ 5, 25, 45,75,100, 400); AW (4 @ 25); LB (5 bottles); Zehr (1 @ 25, DCM)
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s2c23 0800 1000m Karl (1 @ 5, 100, 200, 300, 500, 750, 900, 1000); Zehr (1 @ 25m, 45m); Church (1 @ 5, 25, 45, 75, 100, 125); O2Ar-Karl (1 @ 5, 25, 45, 75, 100); KB (1 @ 25)
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s2c24 1200 400m isopycnal cast: (3 @ 25, 75, 100, DCM, DCM isopyc, NO2 max, 175, 250)
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s2c25 1700 400m biogeochem cast: (3 @ 5, 25, 45, 75, 100, 125, 150, 175)
24
s2c26 2000 400m LB (5 bottles) 5
BEGIN BURST SAMPLING @ 25m, DCM, and DCM isopyc.
August 10 s2c27 0000 400m BurstSamples (2 @ 25, DCM, DCM-iso) 6
s2c28 0400 400m rate cast: ST (4 @ 25); SD, MRM, HA (1 @ 5, 25, 45,75,100, 400); AW (2 @ 25); LB (5 bottles); BurstSamples (2 @ 25, DCM, DCM-iso)
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s2c29 0800 1000m Karl (1 @ 5, 100, 200, 300, 500, 750, 900, 1000); Zehr (1 @ 5, 25, 45, 75, 100, 125, 150, 175); Church (1 @ 5, 25, 45, 75, 100, 125); O2Ar-Karl (1 @ 5, 25, 45, 75, 100); KB (1 @ 25); BurstSamples (2 @ 25, DCM, DCM-iso)
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s2c30 1200 400m isopycnal cast: (3 @ 25, 75, 100, DCM, DCM isopyc, NO2 max, 175, 250)
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s2c31 1400 400m SD/MRM/HA 12 @ 25m 12
s2c32 1700 400m BurstSamples (2 @ 25, DCM, DCM-iso); JM (3 @ 25, 75, 125)
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s2c33 2000 400m BurstSamples (2 @ 25, DCM, DCM-iso); LB (5 bottles) 11
August 11 s2c34 0000 400m BurstSamples (2 @ 25, DCM, DCM-iso); Zehr (1 @ 45m)
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s2c35 0400 400m rate cast: ST (4 @ 25); SD, MRM, HA (1 @ 5, 25, 45,75,100, 400); AW (4 @ 25); LB (4 bottles); BurstSamples (2 @ 25, DCM, DCM-iso)
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s2c36 0800 1000m Karl (1 @ 5, 100, 200, 300, 500, 750, 900, 1000); Zehr (1 @ 25m, 45m); Church (1 @ 5, 25, 45, 75, 100, 125); O2Ar-Karl (1 @ 5, 25, 45, 75, 100); KB (1 @ 25); BurstSamples (2 @ 25, DCM, DCM-iso)
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s2c37 1200 400m isopycnal cast: (3 @ 25, 75, 100, DCM, DCM isopyc, NO2 max, 175, 250)
24
s2c38 1700 400m BurstSamples (2 @ 25, DCM, DCM-iso); NP (9 @ 5m); JM (3 @ 25, 75, 125)
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12
s2c39 2000 400m BurstSamples (2 @ 25, DCM, DCM-iso); LB (5 bottles) 11
END BURST SAMPLING
August 12
s2c40 0000 400m 0
s2c41 0400 400m rate cast: ST (4 @ 25); SD, MRM, HA (1 @ 5, 25, 45,75,100, 400); AW (2 @ 25); LB (5 bottles)
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s2c42 0800 1000m Karl (1 @ 5, 100, 200, 300, 500, 750, 900, 1000); Zehr (1 @ 5, 25, 45, 75, 100, 125, 150, 175); Church (1 @ 5, 25, 45, 75, 100, 125); O2Ar-Karl (1 @ 5, 25, 45, 75, 100); KB (1 @ 25)
17
s2c43 1200 400m isopycnal cast: (3 @ 25, 75, 100, DCM, DCM isopyc, NO2 max, 175, 250)
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s2c44 1400 400m SD/MRM/HA 12 @ 25m 12
s2c45 1700 400m biogeochem cast: (3 @ 5, 25, 45, 75, 100, 125, 150, 175)
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s2c46 2000 400m LB (5 bottles) 5
August 13
s2c47 0000 400m Zehr (1 @ 25m, 45m) 2
s2c48 0800 1000m Karl (1 @ 5, 100, 200, 300, 500, 750, 900, 1000); Zehr (1 @ 25m, 45m); Church (1 @ 5, 25, 45, 75, 100, 125); O2Ar-Karl (1 @ 5, 25, 45, 75, 100); KB (1 @ 25)
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s2c49 1200 400m isopycnal cast: (3 @ 25, 75, 100, DCM, DCM isopyc, NO2 max, 175, 250)
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Ship: R/V KILO MOANA KM12-17 Pumping Date: August 5-14, 2012
Day Pump No.
Time Depth Samples Vol
August 6 1 1400 25m SD/MRM/HA 420L
SA=Samar Abedrabbo HA=Harriet Alexander LB=Lydia Baker BB=Benedetto Barone PB=Paul Berube KB=Karin Björkman MC=Mitchell Chong
TC=Tara Clemente KD=Ken Doggett SD=Sonya Dyhrman RG=Rosie Gradoville RK=Rick Kayser RL=Ricardo Letelier VM=Vanessa Michelou
JM=Jackie Mueller AN=Abigail Noble CP=Cory Padilla NP=Nicole Pereira MRM=Monica Rouco-Molina ST=Sara Thomas AW=Angel White
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SAMPLING OBJECTIVE Isopycnal cast (conducted daily at 1300hrs) 8 depths are sampled on the isopycnal cast: 25m, 75m, 100m, DCM, isopycnal associated with DCM, isopycnal associated with NO2 maximum, 175m, and 250m. Measurement Volume Depths Person Nutrients NO2 0.200 L All CP/MC LLN, NH4,
DON 0.250 L All CP/MC
Gases N2O/CH4 1 L All Karl Pigments HPLC Pigments 5 L 25m, DCM, DCM isopyc TC FCM 0.050 L All KD ATP 3 L All TC DMSP 0.100 L 25m CP 1o productivity O2 Isotopes 0.500 L 25m AW DNA/RNA Prochl. qPCR 0.750 L 25m, 75m, 100m, DCM
isopyc, NO2 max, 175m PB
Microarray 4 L 25m, DCM, DCM isopyc SA/NP DNA/RNA 5 L All VM/PB
Burst sampling (2 niskins @ 25, DCM, DCM isopycnal, every cast for 2 day period). Once per cruise, we will repeat certain measurements conducted @ 25, DCM, DCM isopycnal on the isopycnal cast, on every CTD cast i.e. every 4 hours over a 2 day period. Measurement Volume Depths Person Nutrients NO2 0.200 L 25m
DCM DCM isopyc
Prochl. qPCR on 1200 isopycnal
cast only.
CP/MC LLN, NH4, DON 0.250 L CP/MC Pigments HPLC pigments 5 L TC FCM 0.050 L KD 1o productivity O2/Ar 0.200 L Karl DNA/RNA Prochl. qPCR 0.750 L PB Microarray 4 L SA/NP DNA/RNA 5 L VM/PB
SA=Samar Abedrabbo HA=Harriet Alexander LB=Lydia Baker BB=Benedetto Barone PB=Paul Berube KB=Karin Björkman MC=Mitchell Chong
TC=Tara Clemente KD=Ken Doggett SD=Sonya Dyhrman RG=Rosie Gradoville RK=Rick Kayser RL=Ricardo Letelier VM=Vanessa Michelou
JM=Jackie Mueller AN=Abigail Noble CP=Cory Padilla NP=Nicole Pereira MRM=Monica Rouco-Molina ST=Sara Thomas AW=Angel White
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Biogeochemical cast (conducted every 3 days at 1600hrs) Measurement Volume Depths Person Particulates PC/PN 4 L
5m 25m 45m 75m 100m 125m 150m 175m
TC PPO4 4 L TC Nutrients LLN/LLP/DON 0.750 L TC NO2+NO3,
PO4, Si 0.250 L TC
D15N-NO3 0.250 L TC Gases O2 1 L TC N2O/CH4 1 L Karl Carbon DIC 1.5 L TC DOC 0.250 L TC Pigments Chla –
fluorometry 0.200 L TC
HPLC pigments 5 L TC FCM 0.050 L KD ATP 3 L TC DMSP 0.100 L CP 1o productivity O2/Ar 0.100 L Karl O2 isotopes 0.500 L AW Molecular DNA/RNA 5 L 25m, 75m,
125m, 175m ST (Church)
DNA/RNA 4 L JM (Steward) Rate measurements cast (conducted daily at 0400 hrs) Measurement Volume Depths Person N2 fixation
48 L
25m ST (Church)
1o production by 14C ST (Church) Bacterial production ST (Church) Microarray 4 L 25m, 45m, DCM SA/NP (Zehr) Tricho clades 10 L 5m, 25m, 45m,
75m, 100m SD/MRM/HA (Dyhrman)
Incubation water 10 L 400m SD/MRM/HA (Dyhrman) SA=Samar Abedrabbo HA=Harriet Alexander LB=Lydia Baker BB=Benedetto Barone PB=Paul Berube KB=Karin Björkman MC=Mitchell Chong
TC=Tara Clemente KD=Ken Doggett SD=Sonya Dyhrman RG=Rosie Gradoville RK=Rick Kayser RL=Ricardo Letelier VM=Vanessa Michelou
JM=Jackie Mueller AN=Abigail Noble CP=Cory Padilla NP=Nicole Pereira MRM=Monica Rouco-Molina ST=Sara Thomas AW=Angel White
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Near surface Diaphragm pumping ~25m (conducted at 1400 hrs on 8/6) Measurement Volume Time Person Eukaryotic Metatranscriptomics
60L 45 minutes SD/MRM/HA (Dyhrman)
Incubation water 360L 90 minutes SD/MRM/HA (Dyhrman) SA=Samar Abedrabbo HA=Harriet Alexander LB=Lydia Baker BB=Benedetto Barone PB=Paul Berube KB=Karin Björkman MC=Mitchell Chong
TC=Tara Clemente KD=Ken Doggett SD=Sonya Dyhrman RG=Rosie Gradoville RK=Rick Kayser RL=Ricardo Letelier VM=Vanessa Michelou
JM=Jackie Mueller AN=Abigail Noble CP=Cory Padilla NP=Nicole Pereira MRM=Monica Rouco-Molina ST=Sara Thomas AW=Angel White
It must be noted that at Stn ALOHA there are two existing navigational hazards, known as the WHOTS mooring located at 22o40’ N, 157o57’W and the ALOHA observatory located at 22o44’ N, 158o0.3’W. These moorings are to be avoided by all seagliders, deployed equipment, and ship operations e.g. acoustic interference. Their location, in relation to Stn ALOHA, is provided below.