cancer cells have an altered actin cytoskeleton - cellbiology · normal cell cancer cell cancer...
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Normalcell Cancercell
Cancer cells have an alteredactin cytoskeleton
Oncology Research Unit, Dept Pharmacology, Level 5 Wallace Wurth
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Developing novelchemotherapeutics that target
the actin cytoskeleton•• Drug DevelopmentDrug Development
– Identification of actinspecific target in tumourcells
– Determine the mechanismof action (MOA) of anti-actin compounds :
• Impact on actincytoskeleton
• Cell growth/survival– Determine if this translates
to efficacy against tumourcells anti-actin compoundsare efficacious in tumourcell lines
•• Target ValidationTarget Validation– Changes in the actin
cytoskeleton of cancer cells– Role of the actin
cytoskeleton in cancer cells• Cell growth• Cell survival
– Evaluate what happens totumour cells if specificcomponents of the actincytoskeleton are altered
• siRNA• Knockout animals
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0 6 24 48hrs cAMP
G0/G1
S
G2/GM
Techniques acquired duringhonours
1) Tissue Culture 2) Immunofluorescence staining
3) Western blotting
4) Flow cytometric analysis 5) Viability assays
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Projects: contact details
•• Target validation:Target validation:• Galina Schevzov ([email protected])
•• Anti-actin drug developmentAnti-actin drug development• Justine Stehn ([email protected])