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CAPILLARY ELECTROPHORESIS CAPILLARY ELECTROPHORESIS SYSTEMSSYSTEMS
CAPELCAPEL
CAPILLARY ELECTROPHORESIS SYSTEMSCAPILLARY ELECTROPHORESIS SYSTEMS
CAPELCAPEL
CERTIFICATIONCERTIFICATION
Simultaneous quantitative Simultaneous quantitative determination of several determination of several components in a complex components in a complex mixturemixture
ADVANTAGEADVANTAGESSCAPEL-SERIESCAPEL-SERIES
High separation efficiency Low reagent consumption Simple sample preparation Compact benchtop design Autosamplers (10 inlet and and 10 outlet
vials) in CAPEL-104, CAPEL-104T, and CAPEL-105
Implementation of various methods (Capillary Zone Electrophoresis, Micellar electrokinetic chromatography, Capillaryisoelectric focusing, capillary gel electrophoresis, capillary isotachophoresis)
ELECTROOSMOTIC FLOW (EOF)ELECTROOSMOTIC FLOW (EOF)
R – Si-OH R – Si-O- + H+
K+
A-
EOF
+ _
pH > 3.0
Both anions and cations can be analysed in one run
BASIC FEATURESBASIC FEATURES
CAPEL-103Р, 103РТ
CAPEL-104, 104Т
CAPEL-105
• wavelength 254 nm• autosampler• liquid cooling (model 104Т)
• wavelength 254 nm• manual operation mode• liquid cooling (model 103РТ)
• wavelength 190-380 nm• autosampler• liquid cooling
MAJOR APPLICATIONSMAJOR APPLICATIONS
pesticides inorganic ions phenols
Environment Pharmacology
medicines formulations pharmacokinetics
Food
preservatives dyes target compounds, etc.
Clinical diagnostics proteomics DNA / RNA affinity studies immunoanalyses
Forensic Investigations drugs explosives lethal markers, etc.
Chemical industry
ANALYSIS OF PSYCHOTROPIC ANALYSIS OF PSYCHOTROPIC DRUGSDRUGS
2 3 4 5 6 7
0
5
10
15
Marziali et al., Fig. 3 top
CBZ
EP-CBZ
10,11OH-CBZ
EOF
abso
rban
ce [m
AU
]
time [min]
4 6 8 10 12 14 160
1
2
3
4
Marziali et al, Fig.4, top
EOF
10OH-CBZ
abso
rban
ce [m
AU
]
time [min]
MECK separation of carbamazepine and its main
metabolites
Chiral separation of carbamazepine metabolite
ANALYSIS OF ANTIEPILEPTIC ANALYSIS OF ANTIEPILEPTIC DRUGSDRUGS
3 7 10 130,0
0,2
0,4
0,6
0,8
phe
no
ba
rbita
l
carb
am
aze
pin
e
lam
otr
igin
e
phe
nyt
oin
prim
ido
ne
leve
tira
ceta
mD
MS
O
1-butanol
mA
U
time (min)
3 7 10 130,0
0,2
0,4
0,6
0,8
carb
am
aze
pin
e
lam
otr
igin
e
ph
en
ytoin
ph
en
ob
arb
ital
pri
mid
one
leve
tira
ceta
mD
MS
O
1-propanol
mA
U
time (min)
Microemulsion EKC of 6 closely related antiepileptic drugs
Selectivity changes are seen with different organic modifiers
2 4 6 8 min
mAU
ANALYSIS OF WATER – SOLUBLE ANALYSIS OF WATER – SOLUBLE VITAMINSVITAMINS
Buffer 0.01M borate, pH 9.8Sample standard solution of vitaminsCapillary l/L 50/60 cm, id 75 µmInjection 600 mbar*sVoltage +20 kVDetection 254 nm
1 – vitamin В1
2 – vitamin В12
3 – vitamin В2
4 – vitamin В6
5 – ascorbic acid
6 – nicotinic acid
7 – folic acid
ANALYSIS OF MEDICALLY RELEVANT ANALYSIS OF MEDICALLY RELEVANT PEPTIDESPEPTIDES
5 6 7 8 9 10 11 12 min
1.7 mAU
A
1
2
3
4
1-antithrombine
2-bradikinine
3-angiotensin I
4-angiotensine II
Buffer 10 mM phosphate, pH 2.5Capillary 26 / 35 cm, 50µm IDInjection 150 mbar x secVoltage 25 kVSample 10 µg/mL each
ANALYSIS OF THE CATION COMPOSITION OF ANALYSIS OF THE CATION COMPOSITION OF THE PORTABLE, NATURAL AND WASTE THE PORTABLE, NATURAL AND WASTE
WATERWATER
Buffer 6 mmol benzimidazole, 2.5 mmol tartaric acid, 2.0 mmol 18-crown-6Sample calibration solutionCapillary l/L 50/60 cm, id 75 µmInjection 150 mbar*sVoltage +10 kVDetection 254 nm, indirect
1 – cesium2 – ammonium3 – potassium4 – sodium5 – magnesium6 – lithium7 – strontium8 – barium9 – calcium
ANALYSIS OF THE ANION COMPOSITION OF THEANALYSIS OF THE ANION COMPOSITION OF THEPORTABLE, NATURAL AND WASTE WATERPORTABLE, NATURAL AND WASTE WATER
Buffer 5 mmol chromate, 20 mmol DEA, 1.65 mmol CTABSample calibration solution, 5 mg/l of each anion (fluoride 2.5 mg/l)Capillary l/L 50/ 60 cm, id 75 µmInjection 300 mbar*sVoltage 17 kVDetection 254 nm, indirect
1 chloride2 nitrite3 sulfate4 nitrate5 fluoride6 phosphate* bromide hollow** hydrocarbonate
ANALYSIS OF PESTICIDES OF ANALYSIS OF PESTICIDES OF SYMMETRIC TRIAZINE GROUPSYMMETRIC TRIAZINE GROUP
Buffer 10 mmol sodium borate, 32 mmol SDSSample test solution, 2.5 mg/l of each compoundCapillary l/L 60/70 cm, id 75 µmInjection 900 mbar*sVoltage +20 kVDetection 229 nm
1 – simazine2 – atrazine3 – propazine4 – prometryne
ANALYSIS OF SYNTHETIC ANALYSIS OF SYNTHETIC DYESDYES
Buffer 5 mmol phosphate, 5 mmol hydrocarbonate, pH 10.5Sample standard mixture of dyesCapillary l/L 50/60 cm, id 75 µmInjection 600 mbar*sVoltage +20 kVDetection 254 nm
1 – Rhodamine B (C.I. 45170) 2 – Patent Blue V (E131, C.I. 42051) 3 – Orange I (C.I. 14600) 4 – Acid Green S (E142, C.I. 44090) 5 – Acid Violet 5BN (C.I. 42650) 6 – Quinoline Yellow (E104, C.I. 47005) 7 – Sunset Yellow FCF (E110, C.I. 15985) 8 – Tartrazine (E102, C.I. 19140) 9 – Azorubine (E122, C.I. 14720)10 – Amaranth (E123, C.I. 16185)11 – Chrysoine (C.I. 14270)12 – Brilliant black PN (E151, C.I. 28440)13 – Indigotin (E132, C.I. 73015)14 – Brilliant Blue FCF (E133, C.I. 42090)
ANALYSIS OF BENZOIC ACID ANALYSIS OF BENZOIC ACID DERIVATIVESDERIVATIVES
5 6 7 8 9 10 min
9.86 mAU
A
3-m
eth
ox
yb
enz.
2-m
eth
ylb
enzo
at
ben
zoa
te
ph
tali
c a
cid
Buffer 25 mM borate pH 9.18Capillary 26 / 35 cm, 50µm IDInjection 150 mbar x secVoltage 25 kV
APPLICATIONSAPPLICATIONS
Determination of inorganic anions in water samples
Determination of inorganic cations in water samples
Determination of phenoxycarboxylic acid herbicides in water
Determination of caffeine and preservatives in beverages
SPECIFICATIONSPECIFICATION
Capel-103 Capel-104 Capel-105
DetectorLine-source lamp, 254 nm
Light source - deuterium lamp190 – 380 nm
Operating modeManual
Fully automated,programmable
Capillary coolingAir-forced with ambient t°CIn ver.(T) – thermostatic liquid cooling with pre-set t°C
Liquid cooling with thermostabilisation, from -10 up to +30°C with respect to ambient
Sampler Manually operated for 4 vials
Autosampler, 10 inlet, 10 outlet vials
CONTACTSCONTACTS
LUMEX LTD.LUMEX LTD.
19, Moskovskiy pr., St. Petersburg, 190005 RussiaPhone: +7 (812) 718-5390, +7 (812) 718-5391Fax: +7 (812) 316-6538E-mail: [email protected], [email protected]; http://www.lumex.ru
LUMEX INTERNATIONALLUMEX INTERNATIONAL
4736 Lee Hwy, suite 203, Arlington VA 22207, USAPhone/Fax: +1 (703) 243-7103;E-mail: [email protected]; http://www.lumexint.com
OhioLumex Co.OhioLumex Co.
9263, Ravenna Rd., Unit A3 Twinsburg, OH 44087 USAPhone: +1 (888) 876-2611; +1 (330) 405-0837Fax: +1 (330) 405-0847E-mail: [email protected], [email protected]; http://www.ohiolumex.com