Narrow Sized Dual-functional Microcapsules:

Contact Infection Control and Drug Delivery

Carl Wei He (presenter) Dr. Song Liu

University of Manitoba

Contents

Background Concepts of Microcapsules Applications (esp. in textiles)

Antimicrobial polyurea microcapsules Objective: provide contact wound dressings; Design: Feature and reasoning of the method

Details and characterizations Shell thickness Drug release Contact antimicrobial property

Future research

What is a polyurea microcapsule?

Polyurea

Advances in polyurea microcapsules

medicine

engineering

Traditional applications

Polyurea microcapsules(PUMC)

Electronic ink

Water

treatment

Self-healing

resinTextiles

Agricultures

Artificial blood cells

Targeted/Triggered release

Multi- functional carrier

Application in textiles

Cosmetic textiles

Aromatherapy & Fragrance Textiles

Thermochromic & Photochromic

textiles

Pest repellent textiles

Thermo regulating textiles

Flame retardant textiles

Objective:Antimicrobial microcapsules

Dual functions: On-contact antimicrobial property

To provide an active shell surface Controlled drug release property

To control the thickness of shell

Application Chronic wound care dressing:

infection control and nutrition supply

Requirement for the capusles Morphology:

controlled size and size distribution

Shell: matrix property: Stiff enough to retain spherical shape Thick enough to provide mass regulation

Shell: Surface property: Easily dispersed in water for application Contact-killing effect

Strategy of synthesis Interfactial crosslinking

IR labelling of isocyanate group

Modification by incorporating a multifunctional surfactant

▪ Reduce interface tension▪ Provide antimicrobial functionality▪ Covalently bound to the matrix▪ Increase shell thickness

QAs

Strategy of synthesis functional surfactant

post-modification

in-situ modification

Comparison of two methods model capsules

MC+Q MCQ100a

b

c d

e

fCross-section of fluorescein stained model capsules; left: post-modified model capsule; right: in-situ modified model capsule; (a)(d) freshly prepared model capsule; (b)(e)cracked model capsules after CTAC extraction; (c)(f) close-up of fluorescein stained shell

7.00E-08

1.29E-07

4.99E-07

0.00E+00

1.00E-07

2.00E-07

3.00E-07

4.00E-07

5.00E-07

6.00E-07

Control MCQ100 MC+Q

QAs

den

sity

mol

/cm

2

Model capsules washed against Milli-Q water Before/after CTAC extraction

Before extraction

After extraction

Quantification of QAs density of model capsules. Error bar is generated from 3 batch-to-batch measurements. Control: PUMC model capsule; MCQ100: in-situ modified model capsule; MC+Q: post-modified model capsule;

Up: in-situ modified polyurea microcapsules (MCQ) Down: control polyurea microcapsules (PUMC).

(a) MCQ dispersed in water;

(b) dry MCQ;

(c) PUMC dispersed in water;

(d) dry PUMC

Comparison of two methods microcapsules

In-vitro dissolution test

7-day in-vitro cumulative drug release profile for MCQ-Cmr; insert: first 24hr release curve; (drug load = 3.33 wt%; efficiency = 31%)

Antimicrobial test diffusion

c

ba

MCQ

Control Blank

(a): MCQ, Qas modified capsule;(b): Control, Polyurea capsule without modification;(c): Blank, last batch of washing solution of MCQ sample.

Bacteria load: 4.54 log10 cfu/cm2;Capsule density: 0.88 mg/cm2

Antimicrobial test static contact

0.22 0.44 0.66 0.88

Density of microcapsules mg /cm2

MCQ

Control

Bacteria load: 1.54 log10 cfu /cm2

Antimicrobial test static contact

0.22 0.44 0.66 0.88

Density of microcapsules mg /cm2

MCQ

Control

Bacteria load: 3.54 log10 cfu /cm2

Conclusion

Isocyanate residue on polyurea microcapsules can be utilized for functionalization.

On-contact antimicrobial can be achieved on polyurea microcapsules

Sustained drug release of drug is achieved by thick shell microcapsules.

Polyurea microcapsule of narrow size distribution can be synthesized

Future research nanocapsules

Ease of immobolization Larger surface for functionalization

Future research platform development

Platform for functionalzation Other functional surfactants could be

developted

Future research size control

0 1 2 3 4 5 6 7 8 9 100%

5%

10%

15%

20%

25%

30% diameter distri-bution by volumn

diameter /um

volu

mn d

istr

ibuti

on

rati

o

Acknowledgements

Manitoba Health Research Council (MHRC) Operating and Establishment grants

Dr. Paul H.T. Thorlakson Foundation FundUniversity of Manitoba Research Grant Program (URGP)

Dr. Xiaochen GuDr. Lingdong LiDr. Rick Holley Dr. Saqer Herzallah

Thank you !

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