carlos joão, célia henriques, joão paulo borges, jorge ... · t tissue engineering research at...
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Tissue Engineering Research at the Soft and Biofunctional Materials Group
Carlos João, Célia Henriques, João Paulo Borges, Jorge Carvalho Silva, José Luís Ferreira, Maria do Carmo Lança, Tânia Vieira
Tissue Engineering (TE) is an interdisciplinary field that combines the methods of engineering with the knowledge of the life and exact sciences for the development of functional biological substitutes to improve or replace the function of damaged
or missing organs or tissues.
The tissue engineer’s toolbox comprises biomaterials, cells and regulators of cell function.
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SKIN BONE & CARTILAGE
BLOOD VESSELS SPINAL CORD
TE projects at the SBMG
Microbiological tests with S. epidermidis
PVP+AgNO3 Nanofibers Irradiated with UV for 240 min
Control PCL CS GEL GTA0
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4W Control 4W PCL
4W CS 4W GEL
PCL
CS
GEL
Former students: Ana Espiga, Ana Luísa Marques, Cláudia Aragão, Falk Meutzner, Helena Cardoso, Maike Gomes, Rita Rosa, Susana Gomes, Tiago Valente
In vivo tests with Wistar rats
Wound contraction
Engineered skin substitute - Biomimetic approach: Porous, flexible, multilayered structure: nanofibers Comprising both dermal and epidermal - full skin - equivalents Use of autologous cells (from the patient himself)
PCL
Skin2: a biosynthetic second skin, engineered to treat severe burn wounds PTDC/SAU-BMA/109886/2009, 160 k€
Ag NanoparticlesCS
GEL
CC with 280 µm gelatin/PVA microspheres
PCL ICCs assessed with ATDC5 cells (pre-chondrocytes)
control
Alcian Blue
control
Safranin
PCL ICC, 280 µm pores. 20% PCL solution, freeze-dried
Surface
Pores
DAPI nuclear staining
Bioreactor culture chamber
Chitosan-‐based inverse opals produced from the mesophases of this biopolymer will be able to mimic the structure of the extracellular matrix of bone.
Inverted Colloidal Crystal Scaffolds
Microspheres production and
assembly in a cubic close packed lattice
Infiltration with polymeric liquid
crystalline solutions
Inverse replication by microspheres removal
Histology
In vitro adhesion and proliferation tests
with human dermal fibroblasts
(16±3)µm/h
Biodegradable, hemocompatible, tubular scaffolds made from polymeric nanofibers incorporating topographical cues for directing cell migration and organization
Cell migration along aligned fibers
Average speed: (16±3) µm/h
High speed collector for the production of tubular scaffolds
Aligned PCL fibers
PCL multifibers
Staining for glicosaminoglycans with Safranin and Alcian Blue.
Current students: Carolina Pádua, Constança Garcia, João Miranda, Joana Bianchi, Luísa Fialho, Marc Silva, Margarida Rebelo, Nuno Figueiredo, Vasco Caetano
2D FFT analysis of scaffold anisotropy: frequency plots and alignment plots.
Flan
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Low-cervical lesion in a 24-year-old man with a C4 neurologic level injury (ASIA grade B) sustained after a diving accident. No motor function is preserved below the neu- rological level.
What happens?
Axonal connections are lost.
Diffusion Tensor Tractography image of an injured spinal cord
Can materials help? We are working on that!
Tailoring materials to promote SCI repair
In vitro, primary neurons on P C L / C h i t o s a n a l i g n e d electrospun nanofibers.
N1E-115 cell after 5 days in differentiation medium
Scaffolds may influence neural cells through multiple cues: topographical, mechanical, biochemical and electrical
Scaffolds may support cell transplantation and influence cell fate of stem cells.
Controlling topographic properties of materials to guide and enhance neurite outgrowth
Cylindrical rotating collector with variable rotating speed
250 rpm, chitosan
4000 rpm, chitosan
Control PCL CS GEL GTA0
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60
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100 0 d 1 week 2 weeks 4 weeks
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