cbp/p300-mediated acetylation of histone h3 on lysine 56

CORRIGENDUM doi:10.1038/nature08360 CBP/p300-mediated acetylation of histone H3 on lysine 56 Chandrima Das, M. Scott Lucia, Kirk C. Hansen & Jessica K. Tyler Nature 459, 113–117 (2009) In Fig. 4e of this Letter, one band was incorrectly labelled. We thank B. Lu ¨scher for drawing this to our attention. The correctly labelled version of the figure is provided below. As such, the statement that there is ‘‘a notable correlation between levels of H3K56 acetylation and ASF1A but not ASF1B in a wide variety of normal and cancerous human tissues’’ is no longer accurate. However, the main purpose of Fig. 4e is still valid, which was to show by western blotting analysis that the level of H3K56ac is much higher in some tumours as com- pared to the matching normal tissue. We also note that although the fractionation of the total stained proteins in Figs 1f and 4d did not show obvious differences, the fractionation of histones and tubulin was effective between the super- natant and pellet fractions (see Supplementary Figure). We also omitted to state that the ultraviolet light used in our analysis was 312 nm UVB, which causes 1% of the amount of DNA damage as 254 nm UVC. Supplementary Information is linked to the online version of the paper at www.nature.com/nature. K56Ac ASF1A/B Uterus NTNTNTNTNTNTNT NTNTNTNTNTNTNT Breast Cervix Ovary Testis Prostate Rectum Liver Lung Pancreas Stomach Thyroid Laryngo pharynx Skin K56Ac ASF1A/B GAPDH CORRECTIONS & AMENDMENTS NATUREjVol 460j27 August 2009 1164 Macmillan Publishers Limited. All rights reserved ©2009

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Page 1: CBP/p300-mediated acetylation of histone H3 on lysine 56

CORRIGENDUMdoi:10.1038/nature08360

CBP/p300-mediated acetylation of histoneH3 on lysine 56Chandrima Das, M. Scott Lucia, Kirk C. Hansen & Jessica K. Tyler

Nature 459, 113–117 (2009)

In Fig. 4e of this Letter, one band was incorrectly labelled. We thankB. Luscher for drawing this to our attention. The correctly labelledversion of the figure is provided below. As such, the statement thatthere is ‘‘a notable correlation between levels of H3K56 acetylationand ASF1A but not ASF1B in a wide variety of normal and canceroushuman tissues’’ is no longer accurate. However, the main purpose ofFig. 4e is still valid, which was to show by western blotting analysisthat the level of H3K56ac is much higher in some tumours as com-pared to the matching normal tissue.

We also note that although the fractionation of the total stainedproteins in Figs 1f and 4d did not show obvious differences, thefractionation of histones and tubulin was effective between the super-natant and pellet fractions (see Supplementary Figure).

We also omitted to state that the ultraviolet light used in ouranalysis was 312 nm UVB, which causes 1% of the amount of DNAdamage as 254 nm UVC.

Supplementary Information is linked to the online version of the paper atwww.nature.com/nature.

K56AcASF1A/B

Uterus

N T N T N T N T N T N T N T N T N T N T N T N T N T N T

Breas

Cervix

Ova

Testis

Prostate

Rec

tum