cdc42 interacting protein 4 (cip4), involvement in endocytosis and membrane protrusion
DESCRIPTION
Cdc42 Interacting Protein 4 (CIP4), Involvement in Endocytosis and Membrane Protrusion. Genti Gjyzeli Kelly Mitok Corey O’Reilly. CIP4 Structure. SH3. SH3. FBAR. HR1. HR1. FBAR: Shimada et al., 2007 SH3: Miyamoto et al., unpublished HR1: Kobashigawa et al., 2009. - PowerPoint PPT PresentationTRANSCRIPT
Cdc42 Interacting Protein 4 (CIP4), Involvement in Endocytosis and
Membrane ProtrusionGenti GjyzeliKelly Mitok
Corey O’Reilly
CIP4 Structure
FBARHR1
SH3 SH3
HR1
FBAR: Shimada et al., 2007SH3: Miyamoto et al., unpublishedHR1: Kobashigawa et al., 2009
CIP4 is involved in Endocytosis
FB
FBAR domain
FBAR and HR1 domains
Shimada et al., 2007
Pitts, 2005
Membrane Interacting Residues in Endocytosis
Extracellular
Intracellular
Shimada et al., 2007
CIP4 in Endocytosis
Extracellular
Intracellular
FBAR: Shimada et al., 2007SH3: Miyamoto et al., unpublishedHR1: Kobashigawa et al., 2009
Overexpression of CIP4
Microtubules ActinMicrotubule
Overexpression of CIP4 in neurons gives large protrusion
Normal Cortical Neuron
ActinCIP4
Pictures provided by Dent and Saengsawang
CIP4 Location in Growth Cone
CIP4ActinVideo provided by Dent and Saengsawang
CIP4 Location in Growth Cone
CIP4ActinVideo provided by Dent and Saengsawang
Residues that may be important in protrusion
Extracellular
Intracellular
Shimada et al., 2007
CIP4 in Protrusion
Extracellular
Intracellular
FBAR: Shimada et al., 2007SH3: Miyamoto et al., unpublishedHR1: Kobashigawa et al., 2009
Future Mutation Studies to Determine Important Residues in Protrusion
Shimada et al., 2007
Future Studies to analyze Potential Phosphoregulation of CIP4
Roberts-Galbraith et al., 2010
Diseases Associated with CIP4
• Huntington’s Disease• Cancer
Joshi, 2010
Biocore Instructional Materials• Learning Goals:
– Predict how domains of a protein can influence it’s overall function– Electrostatic interactions of protein with membrane– One protein one function– Predict protein function from location in cell– Endocytosis
• Materials:– Jmol tutorial– Cellular landscape– Animation
Thanks
Michelle HarrisErik DentWitchuda SaengsawangTim, Mark, and Margaret at CBM of MSOEThe other UW-Madison team for their feedback