1.　High adhesive property of animal cells　2.　Promotion of cell spreading　3.　Appropriate for three-dimensional culture

VitrigelⓇ is a registered trademark of National Agriculture and Food Research Organization (NARO).This product is supported by Agri-Health Translational Research Project from the Ministry of Agriculture, Foresty and Fisheries of Japan.

Product No.08363-96

Description 1 plate with lid (12 individual incerts)

Product namead-MED VitrigelⓇ２

Cell Culture Incertad-MED Vitrigelad-MED Vitrigel ® ® 22

High adhesive property of animal cells　2.　Promotion of cell spreading

ad-MED Vitrigel® 2 is a upgraded product from ad-MED Vitrigel®

ad-MED Vitrigel® 2 is a upgraded product from ad-MED Vitrigel®

ad-MED VitrigelⓇ 2 is a cell culture insert using a collagen VitrigelⓇ membrane as a scaffold for cell culturing. ad-MED VitrigelⓇ 2 indicates high adhesive property against various kind of animal cells. Also, it is useful for fabrication of three- dimensional tissue models appropriate for drugs and cosmetics development.

Ordering information

　1.　High adhesive property of animal cellsFeature

Qty. /Pk. 1

High adhesive property of animal cells

ad-MED VitrigelⓇ 2 indicate excellent cell adhesive property as compare to other cell culture inserts using plastic membrane.

ad-MEDVitrigelⓇ 2

ad-MEDVitrigelⓇ

Product A（PET）

Product B（PET）ad-

MED

VitrigelⓇ 2ad-MED

VitrigelⓇ

Product A

（PET） Pro

duct B

（PET）

0

20

40

60

80

100

120（％）

Cell adhesion rate

500 µm

Promotion of cell spreading

ad-MED VitrigelⓇ 2 is promotes rapid cell spreading and forming a focal adhesion.

blue: nucleired: Vinculingreen: Actin20 µm

Incubation time1h 5h1h 5h

ad-MED VitrigelⓇ 2 ad-MED VitrigelⓇ

Cell adhesion rate after 1 hour incubation (cell: HepG2)

Cell morphology (cell: Caco-2)

Appopriate for constructing co-culture systems

Method

ad-MED VitrigelⓇ 2 allow to co-culture different type of cells on both inner and outer side of collagen Vitrigel membrane by using option ring.

Purpose　･Analysis of paracrine effects between two different types of cells 　･Fabrication of tissue models using two different types of cells

Option ring

①Set a option ring to ad-MED VitrigelⓇ 2

②Seed cells to back side

③Remove a option ring and set ad-MED VitrigelⓇ 2 in a multi-well plate

【 Example of use ～ Co-culture of PC-12 and L929 cells ～】Co-culture of PC-12 and L929 cells using a option ring promotes neurite outgrowth of PC-12 cells compared with ordinary co-culture.

0

10

20

30

40

1Co-culture using optionring

Ordinaryco-cultureblue: nuclei red: Neurofilament-L (PC-12)

green: Actin （L929）

Co-culture using option ring 20 µm

Common co-culture

PC-12

PC-12

L929

L929

Left: immunostaining, Right: Comparison of neurite length

neurite length (µm)

Optimize culture systems for fabricating tissue modelsad-MED VitrigelⓇ 2 allows to conduct a variety of cell culture systems in vitro represent environments for cells in vivo.

Product lineup

ReferencesTakezawa T. et al. Cell Transplant. 13: 463-473, 2004.Takezawa T. et al. Toxicol In Vitro. 25: 1237-1241, 2011.Takezawa T. Journal of Biosciences and Bioengineering. 91: 214-217, 2013Yamaguchi H. et al. Toxicol Sci. 135: 347-355, 2013.

Dec. 2016

Liquid phaseculture

Air-liquid interface culture Common co-culture

Co-culture using option ring

※　Please contact us whenever you have questions.

12 well

24 well

96well

ProductName

Number of well 12 well 12 well 24 well 96 well

Aria ofmembrane 1.0 cm2 1.0 cm2 0.33 cm2 0.11 cm2

Quantity ofmembrane

CollagenVitrigel®

Package 12 wells / plate

-

CollagenVitrigel®

CollagenVitrigel®

CollagenVitrigel®

ProductNo.

08360-96 08363-96 08364-96

ad-MEDVitrigel®

ad-MEDVitrigel® 2(12 well)

ad-MEDVitrigel® 2(24 well)

ad-MEDVitrigel® 2(96 well)

KANTO CHEMICAL CO., INC.REAGENT DIVISION

East Muromachi Mitsui BLDG, 2-1, Nihonbashi Muromachi 2-chome, Chuo-ku, Tokyo, 103-0022, JAPAN

+81-3-6214-1092　+81-3-3241-1053

TelephoneTelefax

http://www.kanto.co.jp E-mail; kanto-61@gms.kanto.co.jp

12 wells / plate

24 wells / plate

96 wells / plate