cellmissy: enabling management and dissemination of cell migration data
TRANSCRIPT
![Page 1: CellMissy: enabling management and dissemination of cell migration data](https://reader031.vdocument.in/reader031/viewer/2022030303/587b11451a28abb15c8b670f/html5/thumbnails/1.jpg)
CellMissy: enabling management and dissemination of cell migration data
paola masuzzo
[email protected] / Ghent University, Ghent, Belgium
![Page 2: CellMissy: enabling management and dissemination of cell migration data](https://reader031.vdocument.in/reader031/viewer/2022030303/587b11451a28abb15c8b670f/html5/thumbnails/2.jpg)
A big challenge in cell migrationis data management and dissemination
sample preparation
automated image acquisition
automated image analysis
![Page 3: CellMissy: enabling management and dissemination of cell migration data](https://reader031.vdocument.in/reader031/viewer/2022030303/587b11451a28abb15c8b670f/html5/thumbnails/3.jpg)
A big challenge in cell migrationis data management and dissemination
sample preparation
automated image acquisition
automated image analysis
![Page 4: CellMissy: enabling management and dissemination of cell migration data](https://reader031.vdocument.in/reader031/viewer/2022030303/587b11451a28abb15c8b670f/html5/thumbnails/4.jpg)
There are three key elements to software for cell migration data
Guide and capture experimental set-up
Store, structure and query the acquired data
(Semi-)automate data exploration, analysis and interpretation
![Page 5: CellMissy: enabling management and dissemination of cell migration data](https://reader031.vdocument.in/reader031/viewer/2022030303/587b11451a28abb15c8b670f/html5/thumbnails/5.jpg)
That’s why we developed CellMissy…
CellMissy modules
Experiment
0 3h 6hwound
cells
Experiment Manager
DataLoader
Data Analyzer
Masuzzo et al., Bioinformatics, 2013
![Page 6: CellMissy: enabling management and dissemination of cell migration data](https://reader031.vdocument.in/reader031/viewer/2022030303/587b11451a28abb15c8b670f/html5/thumbnails/6.jpg)
Metadata are crucial
Exchanging the datalab-to-labglobally
Local data management
![Page 7: CellMissy: enabling management and dissemination of cell migration data](https://reader031.vdocument.in/reader031/viewer/2022030303/587b11451a28abb15c8b670f/html5/thumbnails/7.jpg)
Metadata are crucial
Exchanging the datalab-to-labglobally
Local data management
![Page 8: CellMissy: enabling management and dissemination of cell migration data](https://reader031.vdocument.in/reader031/viewer/2022030303/587b11451a28abb15c8b670f/html5/thumbnails/8.jpg)
Detailed metadata annotationis essential for data interpretation
![Page 9: CellMissy: enabling management and dissemination of cell migration data](https://reader031.vdocument.in/reader031/viewer/2022030303/587b11451a28abb15c8b670f/html5/thumbnails/9.jpg)
Detailed metadata annotationis essential for data interpretation
![Page 10: CellMissy: enabling management and dissemination of cell migration data](https://reader031.vdocument.in/reader031/viewer/2022030303/587b11451a28abb15c8b670f/html5/thumbnails/10.jpg)
Detailed metadata annotationis essential for data interpretation
![Page 11: CellMissy: enabling management and dissemination of cell migration data](https://reader031.vdocument.in/reader031/viewer/2022030303/587b11451a28abb15c8b670f/html5/thumbnails/11.jpg)
Image acquisition metadata also tellan important part of the story
Link to raw images (ordering of imaged wells)
Imaging techniques
Exposure time, light intensity, magnification, time interval
![Page 12: CellMissy: enabling management and dissemination of cell migration data](https://reader031.vdocument.in/reader031/viewer/2022030303/587b11451a28abb15c8b670f/html5/thumbnails/12.jpg)
Metadata are crucial
Exchanging the datalab-to-labglobal
Local data management
![Page 13: CellMissy: enabling management and dissemination of cell migration data](https://reader031.vdocument.in/reader031/viewer/2022030303/587b11451a28abb15c8b670f/html5/thumbnails/13.jpg)
Structuring the data takes a bit of effort
![Page 14: CellMissy: enabling management and dissemination of cell migration data](https://reader031.vdocument.in/reader031/viewer/2022030303/587b11451a28abb15c8b670f/html5/thumbnails/14.jpg)
These data support analysis on multiple levels
![Page 15: CellMissy: enabling management and dissemination of cell migration data](https://reader031.vdocument.in/reader031/viewer/2022030303/587b11451a28abb15c8b670f/html5/thumbnails/15.jpg)
These data support analysis on multiple levels
![Page 16: CellMissy: enabling management and dissemination of cell migration data](https://reader031.vdocument.in/reader031/viewer/2022030303/587b11451a28abb15c8b670f/html5/thumbnails/16.jpg)
These data support analysis on multiple levels
![Page 17: CellMissy: enabling management and dissemination of cell migration data](https://reader031.vdocument.in/reader031/viewer/2022030303/587b11451a28abb15c8b670f/html5/thumbnails/17.jpg)
These data support analysis on multiple levels
![Page 18: CellMissy: enabling management and dissemination of cell migration data](https://reader031.vdocument.in/reader031/viewer/2022030303/587b11451a28abb15c8b670f/html5/thumbnails/18.jpg)
These data support analysis on multiple levels
![Page 19: CellMissy: enabling management and dissemination of cell migration data](https://reader031.vdocument.in/reader031/viewer/2022030303/587b11451a28abb15c8b670f/html5/thumbnails/19.jpg)
These data support analysis on multiple levels
![Page 20: CellMissy: enabling management and dissemination of cell migration data](https://reader031.vdocument.in/reader031/viewer/2022030303/587b11451a28abb15c8b670f/html5/thumbnails/20.jpg)
Metadata and data are capturedon all these different levels
![Page 21: CellMissy: enabling management and dissemination of cell migration data](https://reader031.vdocument.in/reader031/viewer/2022030303/587b11451a28abb15c8b670f/html5/thumbnails/21.jpg)
Metadata are crucial
Exchanging the datalab-to-labglobally
Local data management
![Page 22: CellMissy: enabling management and dissemination of cell migration data](https://reader031.vdocument.in/reader031/viewer/2022030303/587b11451a28abb15c8b670f/html5/thumbnails/22.jpg)
Metadata and/or data can be easily exchanged between laboratories
lab A
![Page 23: CellMissy: enabling management and dissemination of cell migration data](https://reader031.vdocument.in/reader031/viewer/2022030303/587b11451a28abb15c8b670f/html5/thumbnails/23.jpg)
Metadata and/or data can be easily exchanged between laboratories
lab A lab B
![Page 24: CellMissy: enabling management and dissemination of cell migration data](https://reader031.vdocument.in/reader031/viewer/2022030303/587b11451a28abb15c8b670f/html5/thumbnails/24.jpg)
Metadata and/or data can be easily exchanged between laboratories
lab A lab B
This is one file in CellMissy! (≈10 MB)
![Page 25: CellMissy: enabling management and dissemination of cell migration data](https://reader031.vdocument.in/reader031/viewer/2022030303/587b11451a28abb15c8b670f/html5/thumbnails/25.jpg)
Metadata are crucial
Exchanging the datalab-to-labglobally
Local data management
![Page 26: CellMissy: enabling management and dissemination of cell migration data](https://reader031.vdocument.in/reader031/viewer/2022030303/587b11451a28abb15c8b670f/html5/thumbnails/26.jpg)
The real target is a global repositoryfor cell migration data
DataRepository
![Page 27: CellMissy: enabling management and dissemination of cell migration data](https://reader031.vdocument.in/reader031/viewer/2022030303/587b11451a28abb15c8b670f/html5/thumbnails/27.jpg)
The real target is a global repositoryfor cell migration data
DataRepository Local Software
![Page 28: CellMissy: enabling management and dissemination of cell migration data](https://reader031.vdocument.in/reader031/viewer/2022030303/587b11451a28abb15c8b670f/html5/thumbnails/28.jpg)
http://cellmissy.googlecode.com
Thank you
Niels Hulstaert prof. dr. Lennart
Martens
prof. dr. Leen Van Troysprof. dr. Christophe
Ampe